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1. 发明公开

KR1020100012319A 패혈증유발 미생물의 분류 및 동정 방법 无效
标题翻译：分类和识别引发微生物的方法
公开(公告)号：KR1020100012319A
公开(公告)日：2010-02-08
申请号：KR1020080073642
申请日：2008-07-28
申请人： 주식회사 씨젠
发明人： 천종윤 , 김종기 , 이대훈 , 이영조
IPC分类号： C12Q1/68
CPC分类号： Y02A50/451 , C12Q1/689 , C12Q1/6827
摘要： PURPOSE: A method for classifying and identifying sepsis-inducing microorganism is provided to rapidly classify and identify the microorganism and prevent generation of error data. CONSTITUTION: A method for classifying and detecting sepsis-inducing microorganism comprises: a step of collecting nucleic acid molecule from a biological sample; a step of contacting primer pair for classifying microorganism group to nucleic acid molecule; a step of amplifying nucleic acid molecule obtained from biological sample through multiplex method; and a step of classifying and detecting sepsis-inducing microorganism. The biological sample is blood, whole blood, or serum.
摘要翻译：目的：提供分类和鉴定脓毒症诱导微生物的方法，以快速分类和鉴定微生物并防止产生错误数据。构成：分类和检测败血症诱导微生物的方法包括：从生物样品中收集核酸分子的步骤; 将引物对与核酸分子分类的步骤; 通过多重方法扩增从生物样品获得的核酸分子的步骤; 以及分类和检测败血症诱导微生物的步骤。生物样品是血液，全血或血清。

2. 发明公开

KR1020090067334A 핵산 중합효소의 뉴클레아제 활성을 이용한 타깃핵산분자의 검출방법 无效
标题翻译：使用核酸聚合酶的核酸酶活性检测目标核酸的方法
公开(公告)号：KR1020090067334A
公开(公告)日：2009-06-25
申请号：KR1020070134955
申请日：2007-12-21
申请人： 주식회사 씨젠
发明人： 천종윤 , 황인택 , 이대훈 , 김종기 , 한진 , 이상길
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6813 , C12Q2563/107
摘要： A method for detecting target sequence in nucleic acid molecule sample using the 3'-5' exonuclease activation of nucleic acid polymerase is provided to detect the target sequence with improved specificity and convenience by amplifying the sequence. A method for detecting a target nucleic acid sequence of nucleic acid sample comprises: a step (i) of hybridizing oligonucleotide which a reporter molecule and quencher molecule are bonded with the nucleic acid sample; a step (ii) of reacting a nucleic acid polymerase having the activity of 3'-5' exonuclease with reaction material of the step (i); and a step of detecting specific fluorescence which is changed by the separation of the reporter molecule and quencher molecule in the reaction material of the step (ii).
摘要翻译：提供使用核酸聚合酶的3'-5'核酸外切酶活化检测核酸分子样品中靶序列的方法，通过扩增序列来检测具有改进的特异性和方便性的靶序列。用于检测核酸样品的靶核酸序列的方法包括：将报道分子和猝灭剂分子与核酸样品结合的寡核苷酸杂交的步骤（i）使具有3'-5'外切核酸酶活性的核酸聚合酶与步骤（i）的反应物质反应的步骤（ii）; 以及通过步骤（ii）的反应材料中报道分子和猝灭剂分子的分离来检测特异性荧光的步骤。

3. 发明授权

KR100881924B1 레이블링된 프라이머를 이용한 뉴클레오타이드 변이 검출방법 有权
标题翻译：使用标记的引物检测核苷酸变异的方法
公开(公告)号：KR100881924B1
公开(公告)日：2009-02-04
申请号：KR1020070024849
申请日：2007-03-14
申请人： 주식회사 씨젠
发明人： 천종윤 , 이영조 , 이대훈 , 김종기
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858
摘要： 본 발명은 타깃 뉴클레오타이드 서열의 최소 2 종류 이상의 뉴클레오타이드 변이를 동시에 검출하는 방법에 관한 것이다. 본 발명의 방법에 따르면, 종래의 부가적인 제한효소 처리나 염기서열분석 과정 없이 간단한 증폭 반응 및 혼성화 과정을 통하여, 2종 이상의 뉴클레오타이드 변이를 오류 없이 동시에 정확하게 검출할 수 있다.
변이, 뉴클레오타이드, 증폭, 프라이머, 혼성화, 프로브

4. 发明公开

KR1020080083949A 레이블링된 프라이머를 이용한 뉴클레오타이드 변이 검출방법 有权
标题翻译：使用标记的PRIMERS检测核酸变异的方法
公开(公告)号：KR1020080083949A
公开(公告)日：2008-09-19
申请号：KR1020070024849
申请日：2007-03-14
申请人： 주식회사 씨젠
发明人： 천종윤 , 이영조 , 이대훈 , 김종기
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6827
摘要： A method for detecting nucleotide variations of a target nucleotide sequence from a sample is provided to detect at least two of the nucleotide variations simultaneously and accurately without error through a simple amplification and a hybridization without a restriction enzyme treatment or a sequence analyzing process. A method for detecting at least two nucleotide variations of a target nucleotide sequence from a sample including the target nucleotide sequence comprises the steps of: (a) hybridizing the target nucleotide sequence with a primer selected from the group consisting of: (i) a first nucleotide variation specific primer 1(NVS-P1) which is specifically hybridized into a variation-occurring region of a target nucleotide sequence having a nucleotide corresponding to a first nucleotide variation, includes a nucleotide complementary to the nucleotide corresponding to the first nucleotide variation and has a detectable mark at a 5'-terminal; (ii) a target specific primer 1(TSP1) which is hybridized into a specific region of a target nucleotide sequence which is located at upstream of the variation-occurring region where the NVS-P1 primer is hybridized; (iii) a second nucleotide variation specific primer 2(NVS-P2) which is specifically hybridized into a variation-occurring region of a target nucleotide sequence having a nucleotide corresponding to a second nucleotide variation at the same site where the first nucleotide variation occurs or an adjacent site thereof, includes a nucleotide complementary to the nucleotide corresponding to the second nucleotide variation and has a detectable mark at a 5'-terminal; and (iv) a target specific primer 2(TSP2) which is located at downstream of the variation-occurring region where the NVS-P2 primer is hybridized; (b) amplifying the target nucleotide sequence through at least two cycles of a primer annealing, a primer elongation and a denaturation; (c) hybridizing an amplification product obtained from the step(b) into a first probe and a second probe, wherein the first probe is specifically hybridized into a product amplified by the primer set(i) and (ii) and the second probe is specifically hybridized into a product amplified by the primer set(iii) and (iv); and (d) detecting a hybridization signal obtained from the step(c), wherein the detectable mark is a chemical mark, an enzyme mark, a radioactivity mark, a fluorescence mark, a luminescent mark, a chemiluminescent mark, an FRET(fluorescence resonance energy transfer) mark or a metal mark.
摘要翻译：提供了从样品中检测靶核苷酸序列的核苷酸变异的方法，通过简单扩增和无限制酶处理或序列分析过程的杂交来同时准确地检测至少两个核苷酸变异。从包含靶核苷酸序列的样品中检测靶核苷酸序列的至少两个核苷酸变异的方法包括以下步骤：（a）使靶核苷酸序列与选自以下的引物杂交：（i）第一与具有对应于第一核苷酸变异的核苷酸的靶核苷酸序列的变异区域特异性杂交的核苷酸变异特异性引物1（NVS-P1）包含与对应于第一核苷酸变异的核苷酸互补的核苷酸，具有 5'-末端的可检测标记; （ii）与位于NVS-P1引物杂交的变异区域的上游的靶核苷酸序列的特定区域杂交的靶特异性引物1（TSP1）; （iii）第二核苷酸变异特异性引物2（NVS-P2），该第二核苷酸变异特异性引物2（NVS-P2）特异性杂交到具有对应于第一核苷酸变异的相同部位的第二核苷酸变异的核苷酸变异的核苷酸序列的变异区域，或其相邻位置包含与对应于第二核苷酸变异体的核苷酸互补的核苷酸，并且在5'-末端具有可检测标记; 和（iv）位于NVS-P2引物杂交的变异区域下游的靶特异性引物2（TSP2）; （b）通过引物退火，引物延伸和变性至少两个循环来扩增靶核苷酸序列; （c）将从步骤（b）获得的扩增产物杂交到第一探针和第二探针中，其中第一探针被特异性杂交到由引物组（i）和（ii）扩增的产物中，第二探针特异性杂交到由引物组（iii）和（iv）扩增的产物中; 和（d）检测从步骤（c）获得的杂交信号，其中可检测标记是化学标记，酶标，放射性标记，荧光标记，发光标记，化学发光标记，FRET（荧光共振能量转移）标记或金属标记。

5. 发明公开

KR1020100048028A 식물 감염성 세균 또는 곰팡이 검출용 올리고뉴클레오타이드 无效
标题翻译：用于检测植物感染细菌或真菌的寡核苷酸
公开(公告)号：KR1020100048028A
公开(公告)日：2010-05-11
申请号：KR1020080107008
申请日：2008-10-30
申请人： 주식회사 씨젠
发明人： 천종윤 , 김종기 , 김태산 , 류기현 , 김욱
IPC分类号： C12N15/00 , C12N15/31
摘要： PURPOSE: An oligonucleotide for detecting plant pathogenic bacteria and fungi is provided to simultaneously amplify a target sequence of bacteria and fungi and to ensure excellent workability in multiplex PCR. CONSTITUTION: An oligonucleotide which is hybridized with plant pathogenic bacteria or fungi is denoted by formula 5'-Xp-Yq-Zr-3'. In the formula, Xp is a 5'-first priming portion having complementary hybridization sequence with a target sequence; Yq is a separation portion containing at least two or more universal bases; Zr is a 3'-second priming portion having complementary hybridization sequence with the target sequence; P, q, and R is the number of nucleotide; and X, Y, and Z is deoxyribonucleotide or ribonuelcotide. The Tm of the 5'-first priming portion is higher than Tm of 3'-second priming portion.
摘要翻译：目的：提供用于检测植物病原菌和真菌的寡核苷酸，以同时扩增细菌和真菌的靶序列，并确保多重PCR中优异的可加工性。构成：与植物病原菌或真菌杂交的寡核苷酸由式5-Xp-Yq-Zr-3'表示。在该式中，Xp是与靶序列具有互补杂交序列的5'-第一引发部分; Yq是含有至少两个以上通用碱的分离部， Zr是具有与靶序列互补杂交序列的3'-第二引发部分; P，q和R是核苷酸的数目; X，Y和Z是脱氧核糖核苷酸或核糖核酸。 5'-第一引发部分的Tm高于3'-第二引发部分的Tm。

6. 发明公开

KR1020090038732A 박과 식물 감염성 바이러스 검출용 올리고뉴클레오타이드 无效
标题翻译：用于检测感染性病毒物种的寡核苷酸
公开(公告)号：KR1020090038732A
公开(公告)日：2009-04-21
申请号：KR1020070104185
申请日：2007-10-16
申请人： 주식회사 씨젠
发明人： 천종윤 , 김종기 , 김태산 , 류기현 , 김욱
IPC分类号： C12N15/11 , C07H21/00
摘要： Oligonucleotide for detecting cucurbitaceous plant-infecting virus is provided to accurately detect seed-infecting virus for cucurbitaceous plant through PCR and electrophoresis by using sequence for virus hybridization having a dual priming oligonucleotid type. Oligonucleotide for detecting cucurbitaceous plant-infecting virus is represented by the formula 1 of 5'-Xp-Yq-Zr-3'. In the formula 1, Xp is a 5'-first priming portion having hybridization sequence complementary to a target sequence to be hybridized; Yq is a separation portion having two or more universal bases; Zr is 3'-second priming portion having hybridization sequence complementary to a target sequence to be hybridized; p, q and r are the number of nucleotides; and X, Y and Z show deoxyribonucleotide or ribonucleotide. Tm of the 5'-first priming portion is higher than the Tm of the 3'-second priming portion.
摘要翻译：提供了用于检测葫芦科植物感染病毒的寡核苷酸，通过使用具有双引发寡核苷酸类型的病毒杂交的序列通过PCR和电泳来准确检测葫芦科植物的种子感染病毒。用于检测葫芦科植物感染病毒的寡核苷酸由5'-Xp-Yq-Zr-3'的式1表示。在式1中，Xp是具有与要杂交的靶序列互补的杂交序列的5'-第一引发部分; Yq是具有两个以上通用基体的分离部; Zr是具有与要杂交的靶序列互补的杂交序列的3'-第二引发部分; p，q和r是核苷酸数; X，Y和Z表示脱氧核糖核苷酸或核糖核苷酸。 5'-第一引发部分的Tm高于3'-第二引发部分的Tm。

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