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    • 3. 发明授权
    • 플로우 사이토미터를 이용한 고상형 면역 측정방법
    • 使用流式细胞仪进行免疫测量
    • KR100119909B1
    • 1997-10-20
    • KR1019930020520
    • 1993-10-05
    • 한국과학기술연구원
    • 김길현한미영윤도영최인성정태화
    • G01N33/53
    • An immunity measuring method using flow cytometer comprising : the step bonding anti-IgG, anti-IgM and anti-IgA to agarose bead as the antibody of the measuring object about the isotype of immune globulin; the step injecting the measuring object into said bead which the antibody is bonded to; the step washing and removing IgG, IgM and IgA which aren't reacted; the step injecting anti-Ig which fluorescent material is attached to and IgG, IgM and IgA can be bonded to; the step washing and removing the injected antibody which isn't reacted; and the step analyzing the bead by the flow cytometer; is disclosed. Thereby, it is possible to use the flow cytometer which is applied in more accurately and wider range than prior immunity measuring method.
    • 一种使用流式细胞仪的免疫测量方法,包括:将抗-IgG,抗-IgM和抗-IgA作为免疫球蛋白同种型的测量对象抗体的琼脂糖珠粘合步骤; 所述步骤将测量对象注入所述抗体结合的珠粒中; 步骤洗涤和除去未反应的IgG,IgM和IgA; 注射荧光材料所附着的抗-Ig的步骤和IgG,IgM和IgA可以结合; 步骤洗涤并除去未反应的注射的抗体; 以及通过流式细胞仪分析珠粒的步骤; 被披露。 因此,可以使用比现有的免疫测定方法更准确,更宽的范围应用的流式细胞仪。