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1. 发明授权

KR100792095B1 Ｌ-페닐알라닌 생산능을 갖는 대장균 변이주로부터유전자조작된 Ｌ-트립토판 생산능을 갖는 재조합 대장균균주 및 이를 이용한 트립토판 제조방법 有权
标题翻译：生产具有原始L-苯乙烯胺生产特性的重组亚硝酸盐的遗传工程化L-谷氨酸，以及使用微生物生产L-酪氨酸的方法
公开(公告)号：KR100792095B1
公开(公告)日：2008-01-04
申请号：KR1020060137650
申请日：2006-12-29
申请人： 씨제이 주식회사
发明人： 주재영 , 최향 , 고은성 , 이지선 , 이진호 , 김소영 , 진창현 , 박영훈
IPC分类号： C12N1/20 , C12P13/22
CPC分类号： C12P13/227 , C12N9/88 , C12N15/52
摘要： A novel recombinant Escherichia coli CJ600 is provided to produce a highly concentrated tryptophan within short period of time without producing L-phenylalanine by removing the tryptophan demanding property from an L-phenylalanine producing Escherichia coli mutated strain and inactivating or mutating many genes. A recombinant Escherichia coli CJ600 derived from a mutant strain of Escherichia coli(deposition no. KFCC 10066) having the L-phenylalanine producing capability is deposited as a deposition no. KCCM 10812P and is characterized in that it has inactivated pheA, trpR, mtr, and tnaAB genes and mutated aroG and trpE genes, wherein the tryptophan demanding property on a chromosome is removed, A method for preparing the recombinant Escherichia coli with the L-tryptophan producing capability comprises the steps of: (a) removing the tryptophan demanding property on the chromosome from genes; (b) inactivating the pheA, trpR, mtr, and tnaAB genes to block the biosynthesis of L-phenylalanine; and (c) mutating the aroG and trpE genes to enforce genes related to the biosynthesis of the L-tryptophan.
摘要翻译：提供了一种新型重组大肠杆菌CJ600，通过从L-苯丙氨酸生产的大肠杆菌突变菌株中除去色氨酸要求性质，并使许多基因失活或突变，在短时间内产生高度浓缩的色氨酸，而不产生L-苯丙氨酸。从具有L-苯丙氨酸生产能力的大肠杆菌突变菌株（沉积编号KFCC 10066）得到的重组大肠杆菌CJ600作为沉积物进行沉积。 KCCM 10812P，其特征在于已经使pheA，trpR，mtr和tnaAB基因失活，并且突变aroG和trpE基因，其中染色体上色氨酸要求性质被去除。一种用L-色氨酸制备重组大肠杆菌的方法生产能力包括以下步骤：（a）从基因中去除染色体上的色氨酸要求性; （b）使pheA，trpR，mtr和tnaAB基因失活以阻断L-苯丙氨酸的生物合成; 和（c）使aroG和trpE基因突变以实施与L-色氨酸的生物合成相关的基因。

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