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    • 5. 发明公开
    • 마 종의 추출물 및 이의 의학적 용도
    • DISCOREA SP提取物 及其医疗用途
    • KR1020080071599A
    • 2008-08-04
    • KR1020087014503
    • 2006-11-15
    • 내셔널 양밍 유니버시티
    • 우롱-춘
    • A61K36/8945A61P19/10
    • A61K36/8945
    • The present invention provides a method for treating osteoporosis in a subject in need of such treatment, including administering orally to the subject an effective amount of an extract product of a DioscoreE species capable of promoting proliferation and differentiation of bone marrow cells in the subject, wherein the Dioscorea species is characterized by a randomly amplified polymorphic DNA (RAPD) fingerprint including 14 DNA bands ranging from 428bp, 452bp, 537bp, 602ba, 723bp, 817bp, 934bp, 1140bp, 1242bp, 1478bp, 1641bp, 1904bp, 2151bp and 2918bp, respectively, when genomic DNA of the Dioscorea species is amplified with a primer of SEQ ID NO: 9, and the extract product is prepared by a process including the steps of: (a) extracting a tuber of the Dioscorea species using an alcohol-based solvent in the presence of acetic acid; (b) subjecting the resultant product obtained in step (a) to a separating treatment to obtain a soluble fraction; and (c) removing solvent from the soluble fraction obtained in step (b) extract fractions of Dioscorea sp.
    • 本发明提供了一种治疗需要这种治疗的受试者的骨质疏松症的方法,包括向受试者口服有效量的能够促进受试者中骨髓细胞增殖和分化的DioscoreE种类的提取物,其中 薯a属特征是随机扩增的多态性DNA(RAPD)指纹,包括分别为428bp,452bp,537bp,602ba,723bp,817bp,934bp,1140bp,1242bp,1478bp,1641bp,1904bp,2151bp和2918bp的14个DNA条带 当利用SEQ ID NO:9的引物扩增薯a属物种的基因组DNA时,通过包括以下步骤的方法制备提取产物:(a)使用醇类溶剂提取薯a属的块茎 在乙酸存在下; (b)使步骤(a)中获得的所得产物进行分离处理以获得可溶性级分; 和(c)从步骤(b)中获得的可溶性级分中除去溶剂,提取薯a属的部分。