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    • 3. 发明公开
    • 식물에서 이형-올리고머 단백질의 생산
    • 在植物中生产异源低聚蛋白
    • KR1020070102570A
    • 2007-10-18
    • KR1020077019434
    • 2006-01-27
    • 아이콘 제네틱스 지엠비에이치
    • 기리츠,아나톨리마릴로네트,실베스터클림유크,빅터클레바,유리
    • C12N15/63C12N15/83C12N15/67
    • C12N15/8251C07K14/59C12N15/8203C12N15/8257C12N15/8258
    • Process of producing in a plant, in plant tissue, or in plant cells a hetero-oligomeric protein comprising at least a first and a second protein subunit, said process comprising expressing in plant cells at least said first and said second protein subunit by (i) providing to said plant, said plant tissue or said plant cells a plus-sense single-stranded RNA viral vector encoding at least said first and said second protein subunit or (ii) providing to said plant, said plant tissue or said plant cells a first and a second plus-sense single-stranded RNA viral vector, said first viral vector encoding at least said first protein subunit, said second viral vector encoding at least said second protein subunit, whereby at least said first viral vector and said second viral vector are non-competing viral vectors.
    • 在植物,植物组织或植物细胞中产生包含至少第一和第二蛋白质亚基的杂低聚蛋白的方法,所述方法包括在植物细胞中至少表达所述第一和第二蛋白质亚基(i )向所述植物,所述植物组织或所述植物细胞提供编码至少所述第一和所述第二蛋白质亚单位的正义单链RNA病毒载体,或(ii)向所述植物提供所述植物组织或所述植物细胞 第一和第二正义单链RNA病毒载体,所述第一病毒载体编码至少所述第一蛋白质亚基,所述第二病毒载体编码至少所述第二蛋白质亚基,由此至少所述第一病毒载体和所述第二病毒载体 是非竞争的病毒载体。
    • 4. 发明公开
    • 식물 바이러스 기반 유도성 발현 시스템
    • 植物基于病毒的诱导表达系统
    • KR1020090029180A
    • 2009-03-20
    • KR1020087027008
    • 2007-05-25
    • 아이콘 제네틱스 지엠비에이치
    • 워너,스테판마릴로넷,실베스터클리뮤크,빅터글레바,유리
    • C12N15/82C12N15/09C12N15/05C12N15/52
    • C12N15/8238
    • A process of producing one or more than one protein of interest, comprising: (a) providing a plant or plant cells comprising a first heterologous nucleotide sequence comprising a nucleotide sequence encoding an RNA replicon, and a first inducible promoter operably linked to said nucleotide sequence encoding said RNA replicon; said RNA replicon not encoding a protein providing for cell-to-cell movement of said RNA replicon in said plant; said RNA replicon encoding a polymerase and said one or more than one protein of interest, said polymerase being adapted for replicating said RNA replicon; and (b) inducing, in said plant or plant cells of step (a), said inducible promoter, thereby producing said one or more than one protein of interest in said plant or plant cells.
    • 一种产生一种或多于一种感兴趣的蛋白质的方法,包括:(a)提供包含第一异源核苷酸序列的植物或植物细胞,所述第一异源核苷酸序列包含编​​码RNA复制子的核苷酸序列,和与所述核苷酸序列可操作地连接的第一诱导型启动子 编码所述RNA复制子; 所述RNA复制子不编码提供所述植物中所述RNA复制子的细胞与细胞运动的蛋白质; 所述RNA复制子编码聚合酶和所述一种或多种感兴趣的蛋白质,所述聚合酶适于复制所述RNA复制子; 和(b)在步骤(a)的所述植物或植物细胞中诱导所述诱导型启动子,从而在所述植物或植物细胞中产生所述一种或多种感兴趣的蛋白质。