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    • 71. 发明公开
    • 여러 종류의 디엔에이 프로브들을 사용한 취약-엑스증후군의 진단 방법
    • 使用各种DNA探针的脆性X综合征的诊断方法
    • KR1020000072201A
    • 2000-12-05
    • KR1020000047468
    • 2000-08-17
    • 김희태
    • 김희태
    • C12Q1/68
    • PURPOSE: Provided is a diagnosis method of Fragile-X-syndrome using various DNA probes, which bind with nucleic acid sequence of FMR1 gene related to fragile-X syndrome. Fragile-X-syndrome is diagnosed accurately in a short time by detecting repeat frequency of CGN(n) and CGG sequences in FMR1 gene of the nucleic acid specimen. CONSTITUTION: A diagnosis method of Fragile-X-syndrome using various DNA probes is characterized by comprising the following steps of: (i)decomposing nucleic acid specimens by treating with DNA restriction enzyme which specifically cleaves the environs of FMR1 gene; (ii)adding DNA probe of sequence CGG(m) and GCC(m) whereto the first label material is adhered(m is 3-10 of integer, the times whose CGG and GCC are repeated) and followed by mixing; (iii)adding 2 kinds of sense and anti-sense DNA probes whereto the second label material capable of binding with internal sequence of FMR1 gene is adhered and followed by mixing; (iv)adding 2 kinds of sense and anti sense DNA probes capable of binding with internal sequence of FMR1 gene, whereto biotin is adhered and followed by mixing; (v)thermal denaturation by heating the nucleic acid associated DNA probes; (vi)reacting so as to bind the denatured nucleic acid with DNA probes specifically; (vii)fixing nucleic acid associated DNA probe on micro well plate by injecting into micro well plate where streptavidin is immobilized on the surface; (viii)removing non binding nucleic acid and DNA probe by washing micro well plate; (ix)measuring the nucleic acid amount which is fixed on micro well plate by reading the second label material; (x)measuring the repeat sequence CGG(n) in nucleic acid specimen by reading the first label material; (xi)enumerating the CGG repeat number n in nucleic acid specimen from the result of (ix) and (x).
    • 目的:提供使用各种DNA探针的脆性-X综合征的诊断方法,其与与脆性-X综合征相关的FMR1基因的核酸序列结合。 通过检测核酸标本的FMR1基因中CGN(n)和CGG序列的重复频率,在短时间内精确诊断易碎X综合征。 构成:使用各种DNA探针的脆性-X综合征的诊断方法的特征在于包括以下步骤:(i)通过用特异性切割FMR1基因周围的DNA限制酶处理来分解核酸标本; (ii)添加序列CGG(m)和GCC(m)的DNA探针,粘附第一标签材料(m为整数3-10,重复CGG和GCC的时间),然后混合; (iii)加入2种有义和反义DNA探针,其中能够与FMR1基因的内部序列结合的第二标记物质粘附并随后混合; (iv)加入2种能与FMR1基因的内部序列结合的有义和反义DNA探针,生物素粘附在其上,然后混合; (v)加热核酸相关DNA探针的热变性; (vi)反应以使变性核酸与DNA探针具体结合; (vii)将微孔板上的核酸相关DNA探针通过注射到表面固定有链霉抗生物素蛋白的微孔板中; (viii)通过洗涤微孔板除去非结合核酸和DNA探针; (ix)通过读取第二标签材料测量固定在微孔板上的核酸量; (x)通过读取第一标签材料测量核酸试样中的重复序列CGG(n); (i)从(ix)和(x)的结果列举核酸标本中的CGG重复数n。
    • 80. 发明公开
    • 용지 정돈 스테플러
    • 面向纸的面料
    • KR1020110052057A
    • 2011-05-18
    • KR1020090108934
    • 2009-11-12
    • 김희태
    • 김희태
    • B25C7/00B25C5/00B25C5/11
    • PURPOSE: A paper aligning stapler capable of boring papers is provided to prevent the scattering of papers, even when rapidly pressing stapler. CONSTITUTION: A paper aligning stapler comprises a top part, a bottom part, and plates. The top part is composed of a push bar and a receiving compartment. The bottom part has a press body and a guide groove. If the push bar pushes, the press body extrudes a first front staple. The guide groove is formed in an anvil to curve the end of the staple which is pushed by the push body. The bottom part supports papers. Two plates are separated from each other toward both sides of the anvil of the bottom part as a width of drawer.
    • 目的:提供能够钻孔的纸对准订书机,以防止纸张的散射,即使在快速按下订书机时也是如此。 构成:纸对齐订书机包括顶部,底部和板。 顶部由推杆和接收室组成。 底部具有压制体和引导槽。 如果推杆推动,压机主体挤出第一个前卡钉。 引导槽形成在砧座上,以弯曲由推动体推动的钉的端部。 底部支持文件。 两个板彼此分开,作为抽屉宽度的底部砧座的两侧。