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    • 77. 发明公开
    • 심해 해저에서 분리된 에스터라제 KTL 7
    • 从深海沉积物中分离出的酯酶KTL 7
    • KR1020120129851A
    • 2012-11-28
    • KR1020120116121
    • 2012-10-18
    • 한국해양과학기술원
    • 이정현이현숙강성균김윤재배승섭전정호임재규김상진권개경차선신
    • C12N9/16C12N15/55C12N15/63
    • PURPOSE: An esterase KTL7 which is separated from the bottom of the sea is provided to enhance activities and to have stability in wide range of temperature. CONSTITUTION: An esterase KTL7 which is separated from the bottom of the sea has amino acid sequence of the sequence number 32(SEQ ID NO:32). A gene ciphering the esterase has the base sequence described in the sequence number 31. A manufacturing method of the esterase gene comprises the following steps: transforming the cells into recombinant vector which includes the esterase of the sequence number 31; culturing the transformed cells; and separating the esterase from the cultured cell. The novel esterase gene is obtained by the following steps: extracting environmental DNA from the deposit of the depth of 1,400 M; building a library by using fosmid vector; and separating the gene from a clone which is active in the tricaprulin flat medium.
    • 目的:提供与海底分离的酯酶KTL7,以增强活性,并在宽温度范围内具有稳定性。 构成:从海底分离出的酯酶KTL7具有序列号32(SEQ ID NO:32)的氨基酸序列。 加密酯酶的基因具有序列号31中描述的碱基序列。酯酶基因的制备方法包括以下步骤:将细胞转化成包含序列号31的酯酶的重组载体; 培养转化细胞; 并从培养的细胞中分离酯酶。 通过以下步骤获得新型酯酶基因:从1400m深度的沉积物中提取环境DNA; 使用fosmid载体构建库; 并将该基因与三权纲平台培养基中活性的克隆分离。