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    • 51. 发明公开
    • 인공간섭 펩티드를 이용한 표적 전사인자 비활성화 방법 및 이의 용도
    • 使用人工小干扰肽诱导转录因子灭活的方法及其用途
    • KR1020140058814A
    • 2014-05-15
    • KR1020120125115
    • 2012-11-07
    • 서울대학교산학협력단
    • 박충모서필준
    • A01H5/00C07K14/415C12N15/29C12N15/82
    • C12N15/8261C07K2/00C07K14/415C12N15/8216C12N15/8217C12N15/8266C12N15/827Y02A40/146
    • The present invention relates to a method for inactivating a target transcription factor using artificial small interfering peptide (a-siPEP) and to a use thereof. In the present invention, a-siPEP which is a truncated form of a transcription factor that forms a dimer through dimerization to control a transcription was prepared, and it was verified that a-siPEP cooperates with the transcription factor to form a heterodimer to inhibit the DNA binding of the transcription factor and the intranuclear transfer of the transcription factor, thereby inactivating a corresponding transcription factor in a protein level. A method for inhibiting the activation of a transcription factor using a-siPEP is a transcriptional control method with high accuracy and efficiency which can inhibit the activation of the transcription factor in a protein level by being substituted for gene knock-out and which can be applied to both dicotyledonous plants and monocotyledonous plants.
    • 本发明涉及使用人造小干扰肽(a-siPEP)灭活靶转录因子的方法及其用途。 在本发明中,制备作为通过二聚化形成二聚体以控制转录的转录因子的截短形式的a-siPEP,并且证实a-siPEP与转录因子配合形成异二聚体以抑制转录因子 转录因子的DNA结合和转录因子的核内转移,从而使蛋白质水平上相应的转录因子失活。 使用a-siPEP抑制转录因子活化的方法是具有高精度和高效率的转录控制方法,其可以通过替代基因敲除来抑制蛋白质水平的转录因子的活化,并且可以应用 双子叶植物和单子叶植物。
    • 57. 发明授权
    • B―BOX 도메인 단백질들 및 이를 이용한 식물의 개화시기 조절방법
    • B盒蛋白质和控制植物花费时间的方法
    • KR101380049B1
    • 2014-04-01
    • KR1020130015043
    • 2013-02-12
    • 경상대학교산학협력단
    • 홍종찬신수영우수경심순애김혜진전수정
    • A01H5/00C12N15/29C12N15/63C12N15/82
    • C12N15/827C07K14/415C12N15/113C12N15/8205C12N15/8216C12N15/8218C12Q1/6895
    • The present invention relates to a usage of BBX30 and BBX31, which are mouseear cress-originated B-Box zinc finger transcription factors. More specifically, the present invention relates to a composition for controlling the flowering time of plants using the protein or a gene encoding the same, a recombinant vector, a transgenic plant, a control method of flowering time of plants, and an identification method of substances for controlling the flowering time of plants. The transgenic plants in which the expression of BBX30 or BBX31 is suppressed according to the present invention are delayed in the flowering time compared to the wild plants. Further, as the proteins are combined to CO (CONSTANS) protein, which is a photopeoriodism flowering control factor, the proteins are anticipated to control the flowering time along with CO. Accordingly, useful transgenic plants are able to be developed by using the control method of the flowering time according to the present invention. In particular, transgenic crops are able to be designed to maximize the harvesting amount, and the control method of the flowering time is able to be used for bio-fuel or as a solution to environmental problems.
    • 本发明涉及BBX30和BBX31的用途,BBX30和BBX31是小鼠水芹来源的B盒锌指转录因子。 更具体地说,本发明涉及使用该蛋白质或编码该蛋白质的基因,重组载体,转基因植物,植物开花时间的控制方法以及物质鉴定方法来控制植物的开花时间的组合物 用于控制植物的开花时间。 与野生植物相比,根据本发明抑制BBX30或BBX31表达的转基因植物的开花时间延迟。 此外,随着蛋白质与CO(CONSTANS)蛋白质组合,其是光热开花控制因子,预期蛋白质与CO一起控制开花时间。因此,有用的转基因植物能够通过使用对照方法开发 根据本发明的开花时间。 特别地,转基因作物能够被设计成使收获量最大化,并且开花时间的控制方法能够用于生物燃料或作为环境问题的解决方案。
    • 58. 发明公开
    • ABI3단백질을 코딩하는 유전자를 포함하는 식물체의 개화 조절용 조성물
    • 用于控制包含基因编码ABI3蛋白的花费时间的组合物
    • KR1020140036066A
    • 2014-03-25
    • KR1020120101641
    • 2012-09-13
    • 포항공과대학교 산학협력단
    • 황일두류호진배원실
    • A01N63/00A01G7/06C12N15/29
    • C12N15/827C07K14/415C12N15/8205G01N33/566G01N2333/415
    • A composition including an ABI3 gene of the present invention and a method of delaying the florescence of a plant have effective florescent retardation effects. In the case of an existing method for delaying florescence, it is difficult to control the delay of florescence since the existing method acts as a method which delays florescence by silencing the expression of a gene promoting florescent, and is difficult to be used in the industry since the production of transgenic plants using a gene silencing method such as RNAi and the like is difficult. The present invention facilitates the delay control of florescence and the production of transgenic plants since the ABI3 gene of the present invention acts as a method delaying florescence when the expression of the ABI3 gene is increased. Accordingly, the present invention can be critically applied for increasing the productivity of crops by delaying or preventing the florescence of plants using vegetative tissues or leaves.
    • 包含本发明的ABI3基因的组合物和延迟植物的荧光的方法具有有效的荧光延迟作用。 在现有的延迟荧光方法的情况下,难以控制荧光延迟,因为现有方法作为通过沉默促进荧光的基因的表达来延缓荧光的方法,并且难以在工业中使用 因为使用基因沉默方法如RNAi等的转基因植物的生产是困难的。 本发明有利于延迟控制荧光和转基因植物的生产,因为当ABI3基因的表达增加时,本发明的ABI3基因作为延迟荧光的方法。 因此,本发明可以通过延迟或防止使用营养组织或叶子的植物的荧光来严格地应用于提高作物的生产力。