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    • 40. 发明公开
    • 신규 프로모터 및 이 프로모터를 사용한 유전자 발현방법
    • 用于调节基因表达的新型促进剂和方法
    • KR1020030072208A
    • 2003-09-13
    • KR1020027014299
    • 2001-04-26
    • 주식회사 바이오리더스다카라 바이오 가부시키가이샤
    • 성문희이승구홍승표서화정
    • C12N15/75
    • C07K14/32C12N15/70C12N15/75
    • PURPOSE: Novel promoter which is capable of expressing a gene with a high level and a method for regulating gene expression using the same are provided, thereby easily and cheaply expressing the gene. CONSTITUTION: An isolated DNA having a base sequence represented by SEQ ID NO: 1 or 2 or its fragment showing a constitutive promoter activity in Escherichia coli or bacteria belonging to the genus Bacillus is provided. An isolated DNA hybridizable with the above DNA and showing a constitutive promoter activity in Escherichia coli or bacteria belonging to the genus Bacillus is provided. A recombinant DNA including the above DNA and a foreign gene is provided. A vector containing at least the above DNA for gene expression is provided. An expression vector containing the above recombinant DNA is provided. A transformed cells carrying the above recombinant DNA or the above expression vector is provided. A process for producing a protein comprises culturing the above transformed cell and collecting the protein from the culture medium. A kit for producing a protein contains at least the above DNA or the above vector for gene expression.
    • 目的:提供能够表达高水平基因的新型启动子和使用其的基因表达调节方法,从而容易且廉价地表达该基因。 构成:提供了具有由SEQ ID NO:1或2表示的碱基序列的分离的DNA或其在大肠杆菌或属于芽孢杆菌属的细菌中显示组成型启动子活性的片段。 提供了与上述DNA杂交的分离的DNA,并在大肠杆菌或属于芽孢杆菌属的细菌中显示组成型启动子活性。 提供了包含上述DNA和外来基因的重组DNA。 提供了至少含有上述用于基因表达的DNA的载体。 提供含有上述重组DNA的表达载体。 提供携带上述重组DNA或上述表达载体的转化细胞。 制备蛋白质的方法包括培养上述转化细胞并从培养基中收集蛋白质。 用于产生蛋白质的试剂盒至少含有上述DNA或上述用于基因表达的载体。