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    • 22. 发明公开
    • 낙엽송충버섯 유래의 면역조절활성을 갖는 세포외다당체
    • 具有来自PHELLINUS PINI的免疫调节活性的外生双酚
    • KR1020040069422A
    • 2004-08-06
    • KR1020030005794
    • 2003-01-29
    • 학교법인 영광학원
    • 송치현양병근정상철조성필
    • A61K36/07A61P37/02
    • A61K36/07A61K31/715A61K2236/11Y10S514/885
    • PURPOSE: Exo-biopolymer having immuno-modulating activity from Phellinus pini is provided, which exo-biopolymer has improved immuno-modulating activity such as antimutagenicity, nitric oxide formation, activation of lysosome in macrophage cells, and division of spleen cell, so that it can be useful for improving immunity of human. CONSTITUTION: The exo-biopolymer having immuno-modulating activity from Phellinus pini has molecular weight of 5 kDa, contains 89.74% of total saccharides and 10.26% of total protein, and has glucose as a main neutral saccharide. The method for isolating the exo-biopolymer having immuno-modulating activity comprises the steps of: centrifuging the Phellinus pini cultured medium at 10,447xg for 20 minutes to obtain the supernatant; adding ethanol into the supernatant in a ratio of 4:1 and reacting them for 24 hours to obtain the precipitate; dissolving the precipitate in distilled water, dialysis of the precipitate dissolved solution and freeze-drying the dialyzed solution.
    • 目的:提供了具有免疫调节活性的外生生物聚合物,其具有改善的免疫调节活性,例如抗真菌性,一氧化氮形成,巨噬细胞中溶酶体的活化和脾细胞的分裂, 可用于提高人的免疫力。 构成:具有免疫调节活性的外生生物聚合物具有5kDa的分子量,含有89.74%的总糖和10.26%的总蛋白质,并以葡萄糖为主要中性糖。 用于分离具有免疫调节活性的外生物聚合物的方法包括以下步骤:以10,447xg离心培养的桑黄培养基20分钟,得到上清液; 以4:1的比例向上清液中加入乙醇,并使其反应24小时,得到沉淀物; 将沉淀物溶解在蒸馏水中,沉淀溶解溶液透析并冷冻干燥透析溶液。
    • 23. 发明公开
    • 한약재가 포함된 클로렐라 액기스 제조방법 및 그에 의한 한약재가 포함된 클로렐라 액기스
    • 氯菊酯和草药提取物的生产和氯氰菊酯和草药提取物
    • KR1020030066482A
    • 2003-08-09
    • KR1020030035158
    • 2003-05-31
    • 조철래박선희
    • 조철래박선희
    • A61K35/68
    • A61K36/05A23L33/105A61K36/232A61K2236/11A61K2300/00
    • PURPOSE: A method of preparing the titled Chlorella extracts containing a medicinal component of herbal extracts beneficial for human health, together with a physiologically active component and a pharmacologically active component of Chlorella is provided. The extracts are removed in an unpleasant smell and thus can be safely drunk by persons of any age, including an infant, a child, an adult or the elderly. CONSTITUTION: Chlorella is cultured using water in an aqua-culture tank for 3 to 10 days, the cultured Chlorella is mixed with herbal plants, glacial acetic acid and Chlorella powder, extracted and then filtered. In the mixing process, 0.05 to 0.2g glacial acetic acid and 0.5 to 2kg Chlorella power are mixed with 1L cultured Chlorella respectively. The herbal plants are any one selected from Angelicae Gigantis Radix, Cinnamomi Cortex, Glycyrrhizae Radix and Polygonatum odoratum, or a combination thereof.
    • 目的:提供含有有益于人类健康的草药提取物的药用成分的小球藻提取物的制备方法,以及小球藻的生理活性成分和药理活性成分。 提取物以令人不快的气味去除,因此可以安全地由任何年龄的人,包括婴儿,儿童,成年人或老年人饮用。 构成:在水培养槽中使用水培养小球藻3至10天,将培养的小球藻与草药,冰醋酸和小球藻粉混合,提取然后过滤。 在混合过程中,将0.05〜0.2g冰乙酸和0.5〜2kg小球藻粉分别与1L培养的小球藻混合。 草本植物是选自当归,,肉桂皮质,甘草和黄芪中的任意一种,或其组合。
    • 24. 发明公开
    • 고수율의 진세노사이드를 안정하게 생산하는 파낙스퀸퀴포리움(미국 진셍)의 캘러스 주와 이러한 고수율의진세노사이드를 안정하게 생산하는 파낙스 퀸퀴포리움의캘러스 주의 발육 방법
    • 稳定的高分子金银花丝(PANAX QUINQUEFOLIUM(AMERICAN GINSENG))和一种用于开发PANAX QUINQUEFOLIUM的这种稳定的高金属离子诱导电路的方法
    • KR1020010047849A
    • 2001-06-15
    • KR1019990052236
    • 1999-11-23
    • 카운슬 오브 사이언티픽 앤드 인더스트리얼 리서치
    • 마더알카나마더아자이쿠마르유니얼기리쉬챈드라팔마에쉬상완라젠더씽
    • A01H4/00
    • A01H4/005A01H4/001A61K36/258A61K2236/11C12N5/0025C12N2500/32C12N2500/34C12Q1/6895
    • PURPOSE: A stable high ginsenoside-yielding callus line of Panax quinquefolium (American ginseng) and a method for developing such stable high ginsenoside-yielding callus line are provided, thereby the ginsenoside can be mass-produced regardless of the tophographical and climatic conditions. CONSTITUTION: A stable high ginsenoside-yielding callus line of Panax quinquefolium has (a) purple pigmentation, (b) growth index of 204.8 to 246.01 in about 45 days of culture, (c) ginsenoside yield of 0.94 to 1.33% f.wt. in about 45 days of culture, (d) yield of Rb group of ginsenosides of 49 to 55% from the crude extract in about 45 days of culture, and (e) yield of Rg group of ginsenoside of 35 to 49% from the crude extract in about 45 days of culture. A method for the development of a stable Panax callus line from root explants comprises the steps of: (a) establishing the aseptic callus cultures from root explants on basal medium modified after Murashige and Skoog medium to obtain primary callus, (b) transferring the primary calli to growth medium for optimal growth, (c) multiplying and maintaining the callus for more than 2 years by regular sub-culturing in growth medium every six to seven weeks under temperature 28 2 deg.C. 1000-2000 lux light intensity and 70 to 80% relative humidity, (d) obtaining the fragile and pale white calli, (e) isolating the high yielding purple pigmented callus occurring as spontaneous variant from 20th sub-culture, (f) enriching the isolated purple pigmented variant through selection and cell aggregate cloning in at least seven subsequent sub-cultures, and (g) proliferating the enriched callus line by using culture medium and incubating the callus culture in continuous light of 3000-4000 lux intensity to obtain high yielding callus line.
    • 目的:提供人参皂甙稳定高人参皂甙系(人参)和开发这种稳定的高人参皂甙生产愈伤组织的方法,无论外观和气候条件如何,人参皂甙都可以批量生产。 构成:人参皂甙稳定的高人参皂苷生产线(a)紫色色素沉着,(b)培养约45天生长指数为204.8〜246.01,(c)人参皂甙的产量为0.94〜1.33% 在大约45天的培养中,(d)在大约45天的培养中从粗提取物中产生的人参皂甙Rb比例为49-55%,(e)从粗产物中得到的Rg组人参皂甙35-49% 提取大约45天的文化。 从根外植体开发稳定的三爪芋线的方法包括以下步骤:(a)在Murashige和Skoog培养基上修饰的基础培养基上从根外植体建立无菌愈伤组织培养物以获得初级愈伤组织,(b)转移初级 愈伤组织生长培养基用于最佳生长,(c)通过在温度28±2摄氏度下每六至七周在生长培养基中定期培养,将愈伤组织倍增并维持2年以上。 (d)获得脆弱和浅白色的愈伤组织,(e)分离出来的第20次亚培养物中发生的自发变体的高产紫色色素愈伤组织,(f)富集 通过在至少七个随后的亚培养物中通过选择和细胞聚集克隆分离紫色色素变体,和(g)通过使用培养基增殖富集的愈伤组织,并以3000-4000勒克司强度的连续光温育愈伤组织培养物以获得高产量 愈伤组织线。
    • 26. 发明公开
    • 락토바실러스(Lactobacillus) 속을 이용하여 생리활성물질을 증대하는 산삼배양근의 배양방법
    • 生姜生物活性物质利用乳酸杆菌培养的根
    • KR1020150000936A
    • 2015-01-06
    • KR1020130073083
    • 2013-06-25
    • 주식회사 씨비엔바이오텍
    • 이연진최현탁전민화
    • A01H4/00A01H1/06
    • A23L33/105A01H4/005A23L33/135A61K36/258A61K2236/11A61K2236/19C12R1/225
    • 본 발명의 일실시예에 따른 락토바실러스(Lactobacillus) 속을 이용한 산삼배양근의 배양방법은, 숙성된 김치로부터 락토바실러스(Lactobacillus) 속을 분리하는 단계; 산삼배양근을 미산성 차아염소산수 30ppm에 10분간 침지하여 살균한 후, 상기 미산성 차아염소산수의 첨가를 통해 파쇄한 산삼배양근 원료를 준비하는 단계; 상기 산삼배양근 5% 대 MRS배지 95%의 제1 순화단계, 상기 산삼배양근 20% 대 MRS배지 80%의 제2 순화단계, 상기 산삼배양근 40% 대 MRS 배지 60%의 제3 순화단계, 및 상기 산삼배양근 100%의 제4 순화단계 순으로 순화시키는 종균 단계; 및 상기 순화된 상기 산삼배양근을 상기 락토바실러스(Lactobacillus) 속으로 배양하는 본배양 단계를 포함한다.
    • 本发明提供了培养的人参根的培养方法,用于通过使用乳杆菌属菌种增加生理活性物质。 根据本发明的实施方案,使用乳杆菌属种培养的人参根培养方法。 包括以下步骤:分离乳杆菌 从泡菜老化 在30ppm的弱酸性次氯酸水中浸泡培养的人参根10分钟,对培养的野参人参根进行灭菌,然后通过加入弱酸性次氯酸水制备培养的人参根的粉碎物质 ; 按照第一纯化方法(培养的野山参根的5%到MRS培养基的95%)的顺序进行培养的野山参根的纯化的种子培养过程,第二纯化过程(培养的野山参20% 根系到80%的MRS培养基),第三个纯化过程(40%的培养的人参根到60%的MRS培养基)和第四个纯化过程(培养的人参根的100%); 并进行培养的野生人参根的主培养方法,所述培养的野参人参根已经通过使用乳杆菌属菌种进行了纯化。