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    • 21. 发明授权
    • 차량 트렁크 부착용 비상 경고판
    • KR101923651B1
    • 2018-11-30
    • KR1020170090565
    • 2017-07-17
    • 최영섭최윤재
    • 최영섭최윤재
    • B60Q1/52B60Q1/30B60Q1/26B60Q1/00
    • 본발명은차량트렁크부착용비상경고판에관한것으로, 차량의트렁크리드에부착하여트렁크리드를개방하면이와동시에경고램프모듈이후방에경고표시를할 수있도록함으로써, 운전자가차량에서하차하지않고트렁크개방조작만하더라도후방차량에비상경고표시를할 수있으며, 단순반사시트이외에 LED 램프를점멸함으로써, 후방차량에대한식별성능을더욱향상시킬수 있어비상상황시운전자의하차에따른안전사고위험을방지함은물론비상경고표시를더욱강화하여 2차사고를예방할수 있고, 조작버튼부를통한사용자의조작에의해경고램프모듈의작동상태를조작할수 있도록함으로써, 날씨, 시간등 외부환경에따라경고램프모듈을최적화된상태로점멸조작할수 있어더욱안전하게사용할수 있으며, 시가잭및 커넥터단자를이용하여차량전원에간편하게연결하여동작시킬수 있어사용편의성을더욱강화시킨차량트렁크부착용비상경고판을제공한다.
    • 23. 发明授权
    • 포유동물의 세포퇴화 관련 유전자의 염기서열
    • PBLUESCRIPT-MDF的基本序列
    • KR100261870B1
    • 2000-08-01
    • KR1019970052591
    • 1997-10-14
    • 최윤재
    • 최윤재이주용고윤성하석호김영민김형기이대연
    • C12N15/12
    • PURPOSE: The base sequence of a gene related to cell degeneration of mammals is provided, which is useful in figuring out the causes of breast cancer and diagnosing the disease more correctly. CONSTITUTION: The base sequence of a gene related to cell degeneration is obtained by the following steps of: i) preparing mammary gland cells; ii) obtaining total RNA from the degenerated cells; iii) making cDNA library; iv) performing mRNA differential display PCR to identify kinds of mRNAs which are differently expressed during the degeneration of mammary gland cells; and v) analyzing base sequences through Northern blot to identify expression pattern of genes; and vi) performing TA-cloning with PCR products using pUC19 vector and pGEM-T vector and sequencing cloned cDNA fragments which are as follows; Mis-1, Mis-2, Mis-3 and Mis-4.
    • 目的:提供与哺乳动物细胞变性有关的基因的碱基序列,可用于确定乳腺癌的病因和诊断疾病。 构成:通过以下步骤获得与细胞变性相关的基因的碱基序列:i)制备乳腺细胞; ii)从退化细胞获得总RNA; iii)制作cDNA文库; iv)进行mRNA差异显示PCR以鉴定在乳腺细胞退化过程中不同表达的mRNA的种类; 和v)通过Northern印迹分析碱基序列以鉴定基因的表达模式; 和vi)使用pUC19载体和pGEM-T载体进行PCR产物的TA克隆,并测序如下克隆的cDNA片段; Mis-1,Mis-2,Mis-3和Mis-4。
    • 24. 发明公开
    • 루미노코쿠스알부스유래의베타-글루코시다제유전자
    • 来自RUMINOCOCCUS ALBUS的BETA-GLUCOSIDASE基因
    • KR1020000031369A
    • 2000-06-05
    • KR1019980047373
    • 1998-11-05
    • 최윤재
    • 최윤재조광근우정희김성찬
    • C12N15/52
    • C12N15/52C12N9/2445C12N15/70
    • PURPOSE: A beta-glucosidase gene sequence to be isolated from Ruminococcus albums is identified, and the expression vector inserting such gene and transformants thereby that are able to grow on the selective medium are obtained by using the genetic engineering techniques to produce a large amount of beta-glucosidase being useful in degrading cellulose. CONSTITUTION: A chromosomal DNA of Ruminococcus albums is cleaved with several restriction enzymes, so that its DNA library is prepared. The interest DNA fragments within the DNA library are inserted into a vector plasmid pUC 19. The gene is cloned through incubation of E. coli transformed by the pUC 19 vector. E. coli transformants by the pUC 19 vector containing the beta-glucosidase gene are isolated, which is determined by blue-colored colonization. After incubation of such isolated vector, the beta-glucosidase activity within the culture is measured.
    • 目的:鉴定从Ruminococcus专辑中分离出的β-葡糖苷酶基因序列,并通过使用遗传工程技术获得插入这种能够在选择培养基上生长的基因和转化体的表达载体,产生大量 β-葡糖苷酶可用于降解纤维素。 构成:用几种限制性酶切割红霉素类的染色体DNA,制备其DNA文库。 将DNA文库中的兴趣DNA片段插入载体质粒pUC19中。通过温育由pUC19载体转化的大肠杆菌来克隆该基因。 通过含有β-葡糖苷酶基因的pUC19载体分离大肠杆菌转化体,其通过蓝色定殖来测定。 在分离的载体孵育后,测量培养物内的β-葡糖苷酶活性。