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    • 26. 发明公开
    • 마그네타이트 입자가 코팅된 핵산 추출용 탄소나노튜브 및 이의 제조방법
    • 碳纳米管涂覆磁性颗粒及其制造方法
    • KR1020140056819A
    • 2014-05-12
    • KR1020120122753
    • 2012-10-31
    • 주식회사 새늘바이오단국대학교 산학협력단
    • 박수민서재원신원상김해원
    • C01B31/02C01G49/02
    • C01B32/168C01G49/02
    • The objective of the present invention is to provide carbon nanotubes for extracting nucleic acids on which magnetite particles, that are excellent in extracting DNA in cells, are coated and a method for producing the same. To achieve the objective, provided is a method for producing magnetite particle coated carbon nanotubes for extracting nucleic acids, comprising: a step of adding carbon nanotubes, which are substituted by an amine group, in a magnetite particle aqueous solution in which magnetic particles are included, and agitating the mixture to produce a first product; a step of dropping the first product in an NaOH solution with concentration of 1.5 M to produce a second product; and a step of centrifuging the second product, collecting the precipitate and drying the precipitate. According to the present invention, the carbon nanotubes are not harmful to the human body, minimizes problems such as environmental pollution, exhibits binding to a target DNA and extracts DNA in high yield, thereby capable of securing a large quantity of sample DNAs required per unit time.
    • 本发明的目的在于提供碳纳米管,其用于提取其上涂覆有细胞中提取DNA的磁铁矿颗粒的核酸,以及其制备方法。 为了达到上述目的,提供了一种生产用于提取核酸的涂覆有磁铁矿颗粒的碳纳米管的方法,包括:在其中包含磁性颗粒的磁铁矿颗粒水溶液中加入被胺基取代的碳纳米管的步骤 并搅拌混合物以产生第一产物; 将第一产物滴加到浓度为1.5M的NaOH溶液中以产生第二产物的步骤; 和离心第二产物的步骤,收集沉淀物并干燥沉淀物。 根据本发明,碳纳米管对人体无害,能够最大限度地减少环境污染等问题,与目标DNA结合,提取DNA高,从而能够确保每单位所需的大量样品DNA 时间。
    • 28. 发明公开
    • 친수성 이온성 액체를 이용한 다공성 구조의 생체 고분자 지지체의 제조방법
    • 一种使用水解离子液体制备多孔结构的生物聚合物薄片的方法
    • KR1020130009480A
    • 2013-01-23
    • KR1020110070633
    • 2011-07-15
    • 단국대학교 산학협력단
    • 김해원신원상이혜영
    • A61L27/56A61L27/14A61K47/30A61L27/38
    • A61L27/56A61K47/30A61L27/14A61L27/38A61L2430/00
    • PURPOSE: A manufacturing method of biopolymer scaffolds is provided to enable to manufacture the biopolymer scaffolds without any additional processes by using the phase separation of the hydrophilic ionic liquid and the hydrophobic biopolymer scaffolds and to control the pore size upon the type of the ionic liquid. CONSTITUTION: A manufacturing method of porous-structured biopolymer scaffolds comprises the first step of mixing a biodegradable polymer, hydrophilic ionic liquid and an organic solvent; the second step of manufacturing gel by evaporating the organic solvent from the mixture; and the third step of extracting hydrophilic ionic liquid from the gel using a protic solvent. The manufacturing method further comprises the step of drying the manufactured porous-structured biopolymer scaffolds. The porous structure is an open structure mutually connected in the form of sphere.
    • 目的:提供生物聚合物支架的制造方法,以便能够通过使用亲水离子液体和疏水性生物聚合物支架的相分离并且根据离子液体的类型来控制孔径,而无需任何额外的方法来制造生物聚合物支架。 构成:多孔结构生物聚合物支架的制造方法包括将可生物降解的聚合物,亲水性离子液体和有机溶剂混合的第一步骤; 通过从混合物中蒸发有机溶剂来制造凝胶的第二步骤; 以及使用质子溶剂从凝胶中提取亲水性离子液体的第三步骤。 制造方法还包括干燥所制造的多孔结构的生物聚合物支架的步骤。 多孔结构是以球体形式相互连接的开放结构。