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21. 发明公开

KR1020130127079A ＷＳＳＶ 및 ＨＰＶ의 동시 진단용 멀티플렉스 프라이머 세트 및 이의 용도 有权
标题翻译：用于同时诊断WSSV和HPV的多重引物组及其用途
公开(公告)号：KR1020130127079A
公开(公告)日：2013-11-22
申请号：KR1020120050715
申请日：2012-05-14
申请人： 부경대학교 산학협력단 , (주)바이오니아
发明人： 최태진 , 김남일 , 박한오
IPC分类号： C12N15/11 , C12Q1/68 , C12Q1/04
CPC分类号： C12Q1/04 , C12Q1/686 , C12Q2537/143 , C12Q2600/16 , G01N33/56983
摘要： The present invention relates to a primer set containing two pairs of primer sets of sequence numbers 1-4 for simultaneously diagnosing white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV); a kit for diagnosing for simultaneously diagnosing WSSV and HPV, containing the primer set; a method for simultaneously diagnosing WSSV and HPV using the primer set; a primer set containing a primer set of sequence numbers 1 and 2 for diagnosing WSSV; and a primer set containing a primer set of sequence numbers 3 and 4 for diagnosing HPV.
摘要翻译：本发明涉及包含两对序列号1-4的引物组的引物组，用于同时诊断白点综合征病毒（WSSV）和肝胰腺细小病毒（HPV）; 用于诊断同时诊断含有引物组的WSSV和HPV的试剂盒; 使用引物组同时诊断WSSV和HPV的方法; 包含用于诊断WSSV的序列号1和2的引物组的引物组; 以及包含用于诊断HPV的序列号3和4的引物组的引物组。

22. 发明公开

KR1020080107926A 수질 측정장치 无效
标题翻译：测量水质量的设备
公开(公告)号：KR1020080107926A
公开(公告)日：2008-12-11
申请号：KR1020070056324
申请日：2007-06-08
申请人： (주)바이오니아
发明人： 윤성진 , 김남일 , 박한오
IPC分类号： G01N33/18
CPC分类号： G01N33/186
摘要： A water quality meter using a detection element is provided to improve the efficiency and precision of detection by using a small fish or a water flea as a detection element and by monitoring the only one individual per an accommodation space. A water quality meter using a detection element comprises water tubs(10, 30) which are provided with a plurality of accommodation spaces(11, 31) for accommodating a detection element separately; an lumination source which is installed at the bottom of the water tubs; heaters(19, 39) which controls the temperature of the water of the water tubs; a camera which is separated from the water tubs by a certain distance to photograph the behaviour of the detection element in the water tubs; a transmittance unit which transmits the information about the behaviour of the detection element photographed from the camera; and a display unit which displays the transmitted information.
摘要翻译：提供了使用检测元件的水质计，通过使用小鱼或水蚤作为检测元件，并且通过仅监控每个住宿空间的个体来提高检测的效率和精度。使用检测元件的水质计量器包括：水桶（10,30），其设置有分别容纳检测元件的多个容纳空间（11,31）; 安装在水桶底部的照明源; 加热器（19,39），其控制水桶的水的温度; 一个与水桶隔开一定距离的照相机，以拍摄水桶中检测元件的行为; 透射单元，其发送关于从照相机拍摄的检测元件的行为的信息; 以及显示单元，显示所发送的信息。

23. 发明公开

KR1020120097793A 블로킹 올리고뉴클레오티드를 포함하는 핫스타트 ＰＣＲ용 조성물 有权
标题翻译：用于包含阻断寡核苷酸的热启动PCR的组合物
公开(公告)号：KR1020120097793A
公开(公告)日：2012-09-05
申请号：KR1020110017226
申请日：2011-02-25
申请人： (주)바이오니아
发明人： 이준희 , 최소라 , 김남일 , 박한오
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/686 , C12Q1/6848 , C12Q2527/101 , C12Q2549/101 , C12Q2527/107 , C12Q2537/161 , C12Q2537/163 , C12Q2525/204 , C12Q2525/186
摘要： PURPOSE: A composition for hot-start PCR is provided to prevent DNA band smearing by primer-dimer formation or non-specific reaction. CONSTITUTION: A composition for hot-start PCR contains a blocking oligonucleotide which is complementary to a primer and has blocked hydroxyl group at 3' end. The melting temperature of the blocking oligonucleotide is lower than the primer having the complementary base sequence. The hydroxyl group site of the blocking oligonucleotide is substituted with substituent except for hydroxyl group. The substituent is C3-Space, C6-space, C12-space, C18-Space, amine, phosphate, DIG, or thiol. The composition additionally contains dye substance which is non-reactive to a template DNA. The dye substance is bromophenol blue, xylene cyanol, bromocresol, or cresol red. [Reference numerals] (AA) PCR reactant mixing step, reverse transcription step; (BB) PCR step; (CC, DD) Template; (EE) Primer + blocking primer; (FF) Primer; (GG) Blocking oligo; (HH) No amplification, DNA polymerase inactivation; (II) Amine; (JJ) Polymerase; (KK) Primer + blocking primer, denaturation; (LL) Blocking oligo; (MM) Denaturation; (NN) Annealing; (OO) Blocking oligo separation; (PP) Amplification; (QQ) Elongation
摘要翻译：目的：提供用于热启动PCR的组合物，以通过引物二聚体形成或非特异性反应来防止DNA带涂抹。构成：用于热启动PCR的组合物含有与引物互补并在3'末端具有封闭的羟基的封闭寡核苷酸。封闭寡核苷酸的解链温度低于具有互补碱基序列的引物。封闭寡核苷酸的羟基位置被除羟基以外的取代基取代。取代基是C3-空间，C6-空间，C12-空间，C18-空间，胺，磷酸盐，DIG或硫醇。该组合物另外含有对模板DNA无反应性的染料物质。染料物质为溴酚蓝，二甲苯氰醇，溴甲酚或甲酚红。（标号）（AA）PCR反应物混合步骤，逆转录步骤; （BB）PCR步骤; （CC，DD）模板; （EE）引物+封端引物; （FF）底漆; （GG）阻断寡核苷酸; （HH）无扩增，DNA聚合酶失活; （二）胺（JJ）聚合酶; （KK）引物+封闭引物，变性; （LL）阻断寡核苷酸; （MM）变性; （NN）退火; （OO）阻断寡聚分离; （PP）扩增; （QQ）伸长率

24. 发明授权

KR101886274B1 메르스 코로나 바이러스의 검출용 조성물 및 이를 포함하는 메르스 코로나바이러스 검출방법 및 키트 有权
公开(公告)号：KR101886274B1
公开(公告)日：2018-08-08
申请号：KR1020170057613
申请日：2017-05-08
申请人： (주)바이오니아 , 대한민국(관리부서 질병관리본부장)
发明人： 양정선 , 김성순 , 김유진 , 김남일 , 임지영 , 김소연 , 차영길 , 양정하 , 연우현
IPC分类号： C12Q1/70 , C12Q1/68
CPC分类号： Y02A50/451 , C12Q1/701 , C12Q1/6806
摘要： 본발명은메르스코로나바이러스의 upE 유전자와 ORF1a 유전자부위의특이적인프라이머와프로브를이용하여실시간 RT-PCR방법에의한메르스코로나바이러스의검출에관한것으로, 본발명에의한메르스코로나바이러스진단방법은일반적인객담검체의전처리방법보다간단하고안전하여, 2차감염위험을감소시킬수 있고, 메르스코로나바이러스에만특이적으로반응하여감염여부를신속하고정확하게확인할수 있는효과가있으며, 검체의핵산추출부터결과분석과정을모니터링할 수있어감염의위험감소를통해검사자의안전성을높일수 있다.

25. 发明授权

KR101412123B1 ＨＰＶ의 실시간 진단용 프라이머 세트 및 이의 용도 有权
标题翻译：用于实时诊断HPV的引物及其用途
公开(公告)号：KR101412123B1
公开(公告)日：2014-06-26
申请号：KR1020120050717
申请日：2012-05-14
申请人： 부경대학교 산학협력단 , (주)바이오니아
发明人： 최태진 , 김남일 , 박한오
IPC分类号： C12N15/11 , C12Q1/68 , C12Q1/04
摘要： 본 발명은 서열번호 3 및 4로 표시된 프라이머 세트를 포함하는 HPV의 실시간 진단용 프라이머 세트, 상기 프라이머 세트를 포함하는 HPV의 실시간 진단용 키트 및 상기 프라이머 세트를 이용하여 HPV를 실시간 진단하는 방법에 관한 것이다. 에 관한 것이다.

26. 发明授权

KR101886272B1 메르스 코로나 바이러스의 검출용 조성물 및 이를 포함하는 메르스 코로나바이러스 검출방법 및 키트 有权
公开(公告)号：KR101886272B1
公开(公告)日：2018-08-08
申请号：KR1020170163683
申请日：2017-11-30
申请人： (주)바이오니아 , 대한민국(관리부서 질병관리본부장)
发明人： 양정선 , 김성순 , 김유진 , 김남일 , 임지영 , 김소연 , 차영길 , 양정하 , 연우현
IPC分类号： C12Q1/70 , C12Q1/68
CPC分类号： Y02A50/451 , C12Q1/701 , C12Q1/6806
摘要： 본발명은메르스코로나바이러스의 upE 유전자와 ORF1a 유전자부위의특이적인프라이머와프로브를이용하여실시간 RT-PCR방법에의한메르스코로나바이러스의검출에관한것으로, 본발명에의한메르스코로나바이러스진단방법은일반적인객담검체의전처리방법보다간단하고안전하여, 2차감염위험을감소시킬수 있고, 메르스코로나바이러스에만특이적으로반응하여감염여부를신속하고정확하게확인할수 있는효과가있으며, 검체의핵산추출부터결과분석과정을모니터링할 수있어감염의위험감소를통해검사자의안전성을높일수 있다.

27. 发明授权

KR101555534B1 전자동 무세포 단백질 제조장비 및 제조방법 有权
标题翻译：自动无细胞蛋白质生产仪器
公开(公告)号：KR101555534B1
公开(公告)日：2015-10-06
申请号：KR1020120138335
申请日：2012-11-30
申请人： (주)바이오니아
发明人： 박한오 , 김종갑 , 한지원 , 조유상 , 김하늘 , 이양원 , 김남일
IPC分类号： C12M1/38 , C12M1/18 , C12M1/24 , C07K1/14
摘要： 본발명은고효율전자동단백질제조장비에관한것으로본 발명의전자동장비는투석막이구비된반응용기가장착되는단백질발현반응부와자기장인가장치를구비한단백질정제부, 멀티웰플레이트장착부, 그리고이들간의물질을이송할수 있는자동피펫장치로구성되어무세포단백질합성을다단계적으로저분자발현용액을투석막을통해교환하여고효율로무세포단백질발현반응을수행하고합성된단백질을친화테그와자성입자를이용하여전자동으로정제하고단백질에적합한완충용액으로투석하는과정을전자동으로수행하여다수의단백질들을간편하게수백마이크로그램수준으로제조할수 있는콤팩트하고경제적인전자동단백질제조장비에관한것이다.

28. 发明公开

KR1020140107989A 친화성 태그가 제거된 단백질의 합성방법 无效
标题翻译：合成无标记蛋白的方法
公开(公告)号：KR1020140107989A
公开(公告)日：2014-09-05
申请号：KR1020130022324
申请日：2013-02-28
申请人： (주)바이오니아
发明人： 박한오 , 김민정 , 한지원 , 박기영 , 김남일
IPC分类号： C07K1/22 , C07K1/34 , C12N15/63
摘要： The present invention relates to a method for synthesizing an affinity tag-free cell free target protein comprising: (a) a step for mixing a template coding a target protein having an enzyme cleavage site with cell lysates; (b) a step for expressing the protein by adding a low molecular weight expression solution to the mixture; (c) a step for attaching a magnetic particle binding to an affinity tag located at the end of the expressed protein; (d) a step for separating the affinity tag and magnetic particle from the expressed target protein by treating a protease recognizing the enzyme cleavage site; and (d) a step for collecting the target protein. The method for synthesizing a protein according to the present invention applies a method for extracting a target protein to a cell free protein expression technique using the magnetic particle conjugated to the affinity tag and the protease recognizing specific sequences so as to perform expression and extraction of the tag-free target proteins at the same time, thereby being useful in terms of production of an original form of a protein without artificial sequences having a negative effect on activation and structure of the protein in producing proteins for the purpose of industries, medicine, and researches.
摘要翻译：本发明涉及一种合成无亲和标记物的无细胞靶蛋白的方法，包括：（a）将编码具有酶切位点的靶蛋白质的模板与细胞裂解物混合的步骤; （b）通过向混合物中加入低分子量表达溶液来表达蛋白质的步骤; （c）附着与位于表达蛋白质末端的亲和标签结合的磁性粒子的步骤; （d）通过处理识别酶切割位点的蛋白质来分离亲和标签和磁性颗粒与表达的靶蛋白质的步骤; 和（d）收集靶蛋白的步骤。根据本发明的合成蛋白质的方法将使用与亲和标签结合的磁性颗粒和蛋白酶识别特异性序列的无细胞蛋白质表达技术提取目标蛋白质的方法进行表达和提取无标签的靶蛋白，因此在生产原始形式的蛋白质方面是有用的，没有人造序列，对于产生蛋白质的活性和结构具有负面影响，用于工业，医药和研究。

29. 发明公开

KR1020140070173A 전자동 무세포 단백질 제조장비 및 제조방법 有权
标题翻译：自动细胞免疫蛋白生产仪器
公开(公告)号：KR1020140070173A
公开(公告)日：2014-06-10
申请号：KR1020120138335
申请日：2012-11-30
申请人： (주)바이오니아
发明人： 박한오 , 김종갑 , 한지원 , 조유상 , 김하늘 , 이양원 , 김남일
IPC分类号： C12M1/38 , C12M1/18 , C12M1/24 , C07K1/14
摘要： The present invention relates to a high-efficiency automatic apparatus for cell-free protein production, and more specifically, to a compact and economical automatic apparatus for protein production, which comprises: a protein expression reaction unit equipped with a reaction container having a dialysis membrane; a protein purification unit having a magnetic field application device; a multi-well plate mount unit; and an automatic pipette device movable between the units, and which can conveniently produce multiple proteins in a level of several hundred microgram by automatically performing steps of: exchanging a low-molecular expression solution through a dialysis membrane to perform a cell-free protein expression reaction at high efficiency, thereby synthesizing cell-free proteins in multi-stages; automatically purifying the synthesized proteins using an affinity tag and magnetic particles; and performing dialysis using a buffer solution suitable for the proteins.
摘要翻译：本发明涉及一种用于无细胞蛋白质生产的高效自动装置，更具体地，涉及一种用于蛋白质生产的紧凑且经济的自动装置，其包括：装备有具有透析膜的反应容器的蛋白质表达反应单元 ; 具有磁场施加装置的蛋白质纯化单元; 多孔板安装单元; 以及可在所述单元之间移动的自动移液管装置，其可以通过自动执行以下步骤方便地产生几百微克水平的多种蛋白质：通过透析膜交换低分子表达溶液以进行无细胞蛋白质表达反应以高效率，从而合成多阶段的无细胞蛋白质; 使用亲和标签和磁性颗粒自动纯化合成的蛋白质; 并使用适用于蛋白质的缓冲溶液进行透析。

30. 发明公开

KR1020130127081A ＨＰＶ의 실시간 진단용 프라이머 세트 및 이의 용도 有权
标题翻译：用于实时诊断HPV的初步设置及其用途
公开(公告)号：KR1020130127081A
公开(公告)日：2013-11-22
申请号：KR1020120050717
申请日：2012-05-14
申请人： 부경대학교 산학협력단 , (주)바이오니아
发明人： 최태진 , 김남일 , 박한오
IPC分类号： C12N15/11 , C12Q1/68 , C12Q1/04
CPC分类号： C12Q1/04 , C12Q1/686 , C12Q1/701 , G01N33/56983
摘要： The present invention relates to a primer set containing a primer set of sequence numbers 3 and 4 for diagnosing HPV in real time, a kit containing the primer set for diagnosing HPV in real-time, and a method for diagnosing HPV using the primer set in real-time.
摘要翻译：本发明涉及包含实时诊断HPV的序列号3和4的引物组的引物组，包含用于实时诊断HPV的引物组的试剂盒，以及使用该引物组进行HPV诊断的方法即时的。

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