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    • 11. 发明公开
    • 유전성 백내장 돌연변이 생쥐 및 그의 제조 방법
    • 具有遗传性催泪虫的突变体小鼠及其制备方法
    • KR1020040029893A
    • 2004-04-08
    • KR1020020060334
    • 2002-10-02
    • 한국화학연구원
    • 송창우조규혁조재우이필수유제권한상섭
    • A01K67/027
    • A01K67/0275A01K2227/105A01K2267/0362C12N15/8509
    • PURPOSE: Provided is a mutant mouse having hereditary cataract observable with naked eyes, which is useful as an excellent cataract model animal in diagnosis, prevention and treatment of cataract. CONSTITUTION: The mutant mouse having hereditary cataract is produced by random mutagenesis using N-ethyl-N-nitrosourea(ENU). Particularly, the mouse is a Mus musculus of a BALB/c line. The method for making the mutant mouse comprises the steps of: administering ENU to a male mouse to cause mutagenesis; hybridizing the mutant mouse with a normal mouse to obtain a first-generation mouse, hybridizing the first-generation mouse with a normal mouse to obtain a second-generation mouse, and counter-hybridizing the first-generation mouse with the second-generation mouse to obtain a third-generation mouse; and screening a mouse showing a cataract symptom from the third-generation mice.
    • 目的:提供一种具有裸眼观察的遗传性白内障的突变型小鼠,可用作白内障诊断,预防和治疗中优良的白内障模型动物。 构成:具有遗传性白内障的突变小鼠通过使用N-乙基-N-亚硝基脲(ENU)的随机诱变产生。 特别地,鼠标是BALB / c线的Mus musculus。 制备突变小鼠的方法包括以下步骤:向雄性小鼠施用ENU以引起诱变; 将突变小鼠与正常小鼠杂交以获得第一代小鼠,将第一代小鼠与正常小鼠杂交以获得第二代小鼠,并将第一代小鼠与第二代小鼠逆化杂交至 获得第三代鼠标; 并筛选显示来自第三代小鼠的白内障症状的小鼠。
    • 13. 发明公开
    • 생물학적 조직에 혈관계를 부여하는 방법
    • 生物组织中提供血管系统的方法
    • KR1020160033180A
    • 2016-03-25
    • KR1020167004061
    • 2014-07-15
    • 고리츠다이가쿠호진 요코하마시리츠다이가쿠
    • 다케베다카노리다니구치히데키다카하시요시노부
    • C12N5/071A01K67/027G01N33/50A61L27/38
    • C12N5/0691A01K67/0271A01K2207/12A01K2267/0325A01K2267/0331A01K2267/035A01K2267/0362A61K35/28A61K35/44A61K49/0008A61L27/3808A61L27/3834A61L27/3886C07D499/21C12N2502/13G01N33/5082
    • 시험관내에있어서, 생물학적조직에혈관계를부여하는방법으로서, 생물학적조직을혈관세포및 간엽계세포와공배양하는것을포함하는, 상기방법. 상기방법에의해, 혈관계가부여된생물학적조직. 상기의생물학적조직을비인간동물에이식하고, 혈관망이구축된조직또는장기로분화시키는것을포함하는, 조직또는장기의제작방법. 상기의생물학적조직을인간또는비인간동물에이식하고, 혈관망이구축된조직또는장기로분화시키는것을포함하는, 조직또는장기의재생또는기능회복방법. 상기의생물학적조직을비인간동물에이식하고, 혈관망이구축된조직또는장기로분화시키는것을포함하는, 비인간키메라동물의제작방법. 상기의생물학적조직, 상기방법으로제작된조직및 장기, 그리고상기방법으로제작된비인간키메라동물로이루어지는군에서선택되는적어도 1 개를사용하여, 약제를평가하는방법. 상기방법에의해, 혈관계가부여된생물학적조직을포함하는, 재생의료용조성물.
    • 本发明提供了一种使用组织在体外构建组织构建体而不依赖于支架材料的方法。 一种将生物组织与血管系统体外整合的方法,包括将生物组织与血管细胞和间充质细胞共培养。 通过上述方法与血管系统整合的生物体组织。 一种制备组织或器官的方法,包括将上述生物组织移植到非人动物中并将生物组织分化成其中构建血管网络的组织或器官。 一种组织或器官的再生或功能恢复的方法,包括将上述生物组织移植到人或非人动物中并将生物组织分化成其中构建血管网络的组织或器官。 一种制备非人嵌合动物的方法,包括将上述生物组织移植到非人动物中,并将生物组织分化成其中构建血管网络的组织或器官。 一种药物评价方法,其特征在于,使用选自上述生物体组织的组中的至少一种,通过上述方法制备的组织或器官,以及通过上述方法制备的非人嵌合动物。 一种用于再生医学的组合物,包括通过上述方法与血管系统整合的生物组织。
    • 14. 发明公开
    • 비공유단백질-2 프로모터 및 루시퍼라아제 리포터 유전자가 형질도입된 형질전환 동물
    • 具有UCP-2促进剂和LUCIFERASE报告基因的转基因动物
    • KR1020100092108A
    • 2010-08-20
    • KR1020090011305
    • 2009-02-12
    • 이화여자대학교 산학협력단
    • 김양하이막순
    • A01K67/027A61P3/04G06F19/12
    • A01K67/0275A01K2227/105A01K2267/0362C12N15/8509
    • PURPOSE: A transformed animal is provided, which transduces uncoupling protein-2 promoter and luciferase reporter gene to produce a transformed fertilized egg originated from the transformed animal. CONSTITUTION: A transformed animal transducing uncoupling protein-2 promoter and luciferase reporter gene includes luciferase showing the bioluminescence reaction and a gene which encrypts more than one selected among the group of: active enzymes green fluorescent protein(GFP) showing the fluorescence reaction; reinforced green fluorescent protein(EGFP); red fluorescent protein(RFP); reinforced red fluorescent protein(ERFP); blue fluorescent protein(BFP); reinforced blue fluorescent protein(EBFP); yellow fluorescent protein(YFP); reinforced yellow fluorescent protein(EYFP); indigo fluorescent protein(CFP); and reinforced indigo fluorescent protein(ECFP).
    • 目的:提供转化的动物,其转导解偶联蛋白-2启动子和荧光素酶报告基因以产生源自转化的动物的转化的受精卵。 构成:转化的动物转导解偶联蛋白-2启动子和荧光素酶报告基因包括显示生物发光反应的荧光素酶和加密多于一种选自以下的基因:活性酶绿色荧光蛋白(GFP),显示荧光反应; 增强绿色荧光蛋白(EGFP); 红色荧光蛋白(RFP); 增强红色荧光蛋白(ERFP); 蓝色荧光蛋白(BFP); 增强蓝色荧光蛋白(EBFP); 黄色荧光蛋白(YFP); 强化黄色荧光蛋白(EYFP); 靛蓝荧光蛋白(CFP); 和增强靛蓝荧光蛋白(ECFP)。
    • 18. 发明公开
    • 칼슘 이온 통로 알파1D 유전자 변이 생쥐 및 그의 제조방법
    • 钙离子通道1号转基因小鼠及其生产方法
    • KR1020020089855A
    • 2002-11-30
    • KR1020010028803
    • 2001-05-25
    • 주식회사 오리엔트바이오주식회사 제넥신
    • 신희섭남궁윤
    • A01K67/027
    • A01K67/0275A01K2227/105A01K2267/0362C12N15/8509C12N2015/8563
    • PURPOSE: A calcium ion passage alpha 1 d transgenic mouse and a production method thereof are provided to achieve an animal model for the study of a complication of noninsulin-dependent diabetes mellitus, and so on. CONSTITUTION: The transgenic mouse is homozygote transgenic mouse having a genotype of calcium ion passage alpha 1 D -/- not manifesting calcium ion passage alpha 1 D protein. More particularly, the transgenic mouse is mus musculus. The producing method of the transgenic mouse comprises the steps of; transfecting a targeting vector about the calcium ion passage alpha 1 D gene to mouse embryonic stem cells; attaining a chimera mouse by injecting the embryonic stem cells into blastocoel; attaining a heterozygote mouse having a calcium ion passage alpha 1 D +/- genotype by mating the chimera mouse with a normal mouse; and completing a homozygote transgenic mouse having the calcium ion passage alpha 1 D -/- genotype by mating female and male heterozygote mice with each other.
    • 目的:提供钙离子通道α1d转基因小鼠及其制备方法,以实现用于研究非胰岛素依赖性糖尿病并发症的动物模型等。 构成:转基因小鼠是纯合子转基因小鼠,其具有钙离子通道α1 D - / - 不表现钙离子通道α1D蛋白的基因型。 更具体地说,转基因小鼠是小鼠。 转基因小鼠的制备方法包括以下步骤: 将关于钙离子通道α1D基因的靶向载体转染到小鼠胚胎干细胞; 通过将胚胎干细胞注射到胚泡中获得嵌合体小鼠; 通过将嵌合体小鼠与正常小鼠交配获得具有钙离子通道α1+/- +/-基因型的杂合子小鼠; 并通过将雌性和雄性杂合子小鼠相互交配来完成具有钙离子通道α1D - / - 基因型的纯合子转基因小鼠。