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    • 13. 发明公开
    • 메타게놈 유래 신규 저온성 에스터라제
    • 新的基因编码低温适应性酯酶
    • KR1020120036635A
    • 2012-04-18
    • KR1020100098414
    • 2010-10-08
    • 한국화학연구원
    • 송재광유은영이미애권민아최지은송봉근
    • C12N9/16C12N15/55C12N15/63C12P21/02
    • C12N9/16C07K2319/20C07K2319/50
    • PURPOSE: A novel psychrophilic esterase is provided to ensure high activity at pH 8-10 and to be used in various industries. CONSTITUTION: A polypeptide with esterase activity is a polypeptide: which is encoded by a polynucleotide of sequence number 5; which is encoded by a polynucleotide hybridizable with a polynucleotide of sequence number 5 under a strict condition; or which has an amino acid sequence of sequence number 3. A method for preparing a recombinant esterase comprises: a step of preparing a recombinant expression vector containing the polynucleotide with esterase activity; a step of introducing the recombinant expression vector to a host cell to prepare a transformant; and a step of culturing the transformant to express the recombinant proteins and collecting the recombinant proteins.
    • 目的:提供一种新颖的嗜酸性酯酶,以确保pH 8-10的高活性,并用于各种行业。 构成:具有酯酶活性的多肽是多肽,其由序列号5的多核苷酸编码; 其由在严格条件下可与序列号5的多核苷酸杂交的多核苷酸编码; 或其具有序列号3的氨基酸序列。制备重组酯酶的方法包括:制备含有具有酯酶活性的多核苷酸的重组表达载体的步骤; 将重组表达载体导入宿主细胞以制备转化体的步骤; 以及培养转化体以表达重组蛋白并收集重组蛋白的步骤。