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    • 117. 发明公开
    • 종양-관련 대식세포에 대한 면역 반응을 유발하기 위한 DNA 조성물
    • 针对肿瘤相关巨噬细胞的免疫反应的DNA组合物
    • KR1020090092271A
    • 2009-08-31
    • KR1020097009367
    • 2007-10-05
    • 더 스크립스 리서치 인스티튜트
    • 레이스펠드랄프에이.시앙룽뤄윈핑
    • A61K48/00C12N15/11A61P35/00C12N15/85
    • C12N9/6472A61K39/00A61K39/0011A61K2039/521A61K2039/5254A61K2039/53A61K2039/542A61K2039/645C12N9/50
    • A DNA composition useful for eliciting an immune response against tumor-associated macrophages comprises a DNA construct that encodes for a cysteine endopeptidase (e.g., legumain) or a polypeptide comprising at least one immunogenic fragment thereof that is overexpressed in tumor-associated cells such as tumor-associated macrophages, or an immunogenic fragment thereof that is expressible in immune cells, and which is incorporated in a pharmaceutically acceptable carrier. In preferred embodiments, the composition encodes a polypeptide comprising a plurality of immunogenic fragments of a tumor associated cysteine endopeptidase (e.g., legumain) joined together serially such that each fragment is connected to at least one other fragment by a linker peptide such as AIaAIaAIa or AlaAlaTyr. In one preferred embodiment, the composition also includes a DNA construct that encodes an immune effector protein, such as a cytokine. The DNA composition of the invention can be used alone or in combination with a chemotherapeutic agent to treat diseases such as tumors and tumor metastases. ® KIPO & WIPO 2009
    • 可用于引发针对肿瘤相关巨噬细胞的免疫应答的DNA组合物包含编码半胱氨酸内肽酶(例如,legumain)的DNA构建体或包含其在肿瘤相关细胞如肿瘤中过表达的至少一种免疫原性片段的多肽 相关巨噬细胞或其免疫原性片段,其在免疫细胞中可表达,并且其掺入药学上可接受的载体中。 在优选的实施方案中,组合物编码包含多个连续连接在一起的肿瘤相关半胱氨酸内肽酶(例如,legumain)的免疫原性片段的多肽,使得每个片段通过接头肽如AlaAaAaA或AlaAlaTyr连接至至少一个其它片段 。 在一个优选的实施方案中,组合物还包括编码免疫效应蛋白(例如细胞因子)的DNA构建体。 本发明的DNA组合物可以单独使用或与化学治疗剂组合使用以治疗诸如肿瘤和肿瘤转移的疾病。 ®KIPO&WIPO 2009