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    • 120. 发明公开
    • 신균주 린박터 사르코도니아 엠케이1과 이 균주가 생산하는 거식세포 자극활성을 지닌 체외다당류성 물질
    • 雀形杆菌MK1和雀斑杆菌MK1产生的胞外多糖
    • KR1020120127758A
    • 2012-11-26
    • KR1020110044259
    • 2011-05-11
    • 이영남
    • 이영남
    • C12N1/20C12P19/04C12Q1/02
    • PURPOSE: A Lynbacter sarcodonia MK1 and a method for isolating and purifying exopolysaccharides using the same are provided to ensure physiological activity and to develop a strain which produces physiologically active substances. CONSTITUTION: A Lynbacter sarcodonia MK1(deposit number KCTC 11802BP) produces exopolysaccharides which accelerates immune-modulating physiological activity of macrophage. The strain is isolated from bacterial soft rot region of Sarcodon aspratus. A method for isolating and purifying exopolysaccharides which is secreted to the out of body comprises: a step of culturing the strain; a step of centrifuging strain culture liquid and collecting the supernatant; a step of adding anhydrous ethanol and centrifuging exopolysaccharides; a step of dissolving the exopolysaccharides in distilled water; a step of repeating ethanol precipitation 2-3 times and freeze-drying at -50 Deg. C.; a step of dissolving the exopolysaccharides in distilled water and performing dialysis; and a step of precipitating the strain polysaccharides with cold anhydrous ethanol.
    • 目的:提供菱杆菌MK1和使用其分离和纯化外多糖的方法以确保生理活性并产生产生生理活性物质的菌株。 构成:雀形杆菌MK1(保藏号KCTC 11802BP)产生外多糖,加速巨噬细胞的免疫调节生理活性。 该菌株与沙门氏菌的细菌软腐病区分离。 分离和纯化分泌到体外的多糖的方法包括:培养菌株的步骤; 离心菌液培养液并收集上清液的步骤; 加入无水乙醇和离心多糖的步骤; 将外多糖溶解在蒸馏水中的步骤; 重复乙醇沉淀2-3次并在-50度下冷冻干燥的步骤。 C。; 将外多糖溶解在蒸馏水中并进行透析的步骤; 并用冷无水乙醇沉淀菌株多糖的步骤。