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91. 发明授权

KR101020874B1 해조류 포자 착생 촉진을 위한 캡슐화 조성물 有权
标题翻译：用于促进海洋生物附着和生长的藻类孢子的封闭组合物
公开(公告)号：KR101020874B1
公开(公告)日：2011-03-09
申请号：KR1020080112954
申请日：2008-11-13
申请人： 에스엠텍 주식회사
发明人： 박남식 , 이한성 , 정상목
IPC分类号： A01K61/00 , C12N1/20 , C12N11/04
CPC分类号： Y02A40/81
摘要： 본 발명은 해조류 포자 착생 촉진을 위한 캡슐화 조성물에 관한 것으로서, 더욱 상세하게는 캡슐화제로 알긴산염(alginate salt)을 이용하여 해조류 포자(algae spore)를 혼합한 후 다가금속염을 함유한 알칼리 수용액으로 알긴산염을 경화함으로써 조류 포자를 장기간 생존 보존시킬 수 있으며, 또한 응용분야에서 조류 포자를 구조물에 조기 착생 촉진시킬 수 있는 해조류 포자 착생 촉진용 캡슐화 조성물 및 이를 이용한 해양구조물 표면의 도포방법에 관한 것이다.
미세조류, 해조류 포자, 착생 촉진, 캡슐화, 알긴산염, 다가금속염

92. 发明公开

KR20100106905A Method for the differentiation of stem cells into vascular endothelial cells and induction of angiogenesis using the same 有权
标题翻译：将干细胞分化为血管内皮细胞的方法和使用该方法诱导血管生成
公开(公告)号：KR20100106905A
公开(公告)日：2010-10-04
申请号：KR20100002149
申请日：2010-01-11
申请人： KOREA INST SCI & TECH
发明人： KIM SANG HEON , KIM SOO HYUN , PARK IN SU , JUNG YOUNG MEE
IPC分类号： C12N5/071 , A61K35/12 , C12M3/00 , C12N11/04
CPC分类号： C12N5/069 , A61K35/28 , A61K35/30 , A61K35/44 , A61K35/51 , A61K35/545 , C12N2501/115 , C12N2506/1384 , C12N2513/00 , C12N2533/52 , C12N2533/54 , C12N2533/70
摘要： PURPOSE: A method for differentiating to vascular cells by culturing stem cells in three-dimensional cell cluster is provided to overexpress vascularization promoting factor and to efficiently induce the differentiation. CONSTITUTION: A method for differentiating stem cells to vascular cells comprises: a step of adhering stem cells on a culture container of which surface has hydrophobicity; a step of detaching stem cells from the container and forming three dimensional cell cluster; a step of proliferating the cells in three dimensional cell cluster; and a step of differentiating to vascular cells. A composition for treating vascular diseases or wound contains the cell cluster and culture medium thereof as an active ingredient.
摘要翻译：目的：提供通过在三维细胞簇中培养干细胞来分化为血管细胞的方法，以过表达血管促进因子并有效诱导分化。构成：将干细胞分化为血管细胞的方法包括：将干细胞粘附在表面具有疏水性的培养容器上的步骤; 从容器中分离干细胞并形成三维细胞簇的步骤; 在三维细胞簇中增殖细胞的步骤; 和分化为血管细胞的步骤。用于治疗血管疾病或伤口的组合物含有作为活性成分的细胞簇和培养基。

93. 发明公开

KR1020100044173A 줄기 세포에 대해 펩타이드 연결된 세포 기질 및 이의 사용 방법 无效
标题翻译：用于干细胞的肽连接细胞矩阵材料及其使用方法
公开(公告)号：KR1020100044173A
公开(公告)日：2010-04-29
申请号：KR1020107000802
申请日：2008-06-13
申请人： 에프엠씨 코포레이션
发明人： 브린크만잔엔겔센 , 프론스달카트린브조네벡 , 멜비크잔에질
IPC分类号： C12N11/04 , C12N5/0775 , A61K35/12 , A61P25/00
CPC分类号： C12N5/0663 , A61K2035/128 , C12N5/0667 , C12N2533/50 , C12N2533/74
摘要： Biostructures that comprises modified alginates entrapping one or more stem cells are discloses. The modified alginates comprise at least one alginate chain section to which is bonded by covalent bonding at least one cell attachment peptide. Pluralities of stem cells are also disclosed. Methods of preventing death of stem cells and cells differentiated there from are disclosed. Methods of preparing a plurality of stem cells are disclosed. Methods of treating an individual who has a degenerative disease, such as a neurological disorder, or injury involving nerve damage by administering stem cells to said individual are disclosed.
摘要翻译：公开了包含一个或多个干细胞的修饰藻酸盐的生物结构。经修饰的藻酸盐包含至少一个海藻酸盐链段，通过共价键合至少一个细胞附着肽将其结合。还公开了多种干细胞。公开了预防干细胞和细胞分化的细胞死亡的方法。公开了制备多个干细胞的方法。公开了通过向所述个体施用干细胞来治疗具有退行性疾病如神经障碍或涉及神经损伤的损伤的个体的方法。

94. 发明公开

KR1020070064280A 포괄고정화 담체의 보관방법 및 제조방법 无效
标题翻译：沉积固定介质的储存和制造方法
公开(公告)号：KR1020070064280A
公开(公告)日：2007-06-20
申请号：KR1020060128296
申请日：2006-12-15
申请人： 가부시키가이샤 히타치플랜트테크놀로지
发明人： 아오야마,코우타로우 , 아베,나오키 , 스미노,타츠오 , 에모리,히로요시
IPC分类号： C02F3/10 , C12N11/04 , C12N11/08
CPC分类号： C12N11/04 , C02F3/108 , C12N11/08 , Y02W10/15
摘要： Storage and production methods of encapsulated immobilization carrier which can stably store and transport a carrier block, and can obtain encapsulated immobilization carrier having high quality stability are provided. In a method for storing encapsulated immobilization carrier(30) in which microorganisms are encapsulated and immobilized in an immobilizing material until the encapsulated immobilization carrier is used in a treatment tank, the method comprises: storing a large-sized carrier block(28) in water of 15 deg.C or less or in the atmosphere with a relative humidity of 90% or more and a temperature of 15 deg.C or less before cutting the carrier block into a used size of the encapsulated immobilization carrier until the encapsulated immobilization carrier is used. A method for producing encapsulated immobilization carrier(30) in which microorganisms are encapsulated and immobilized in an immobilizing material, the method comprises: polymerizing a liquid mixture in which the microorganisms are mixed with the immobilizing material into a gel to form a carrier block(28) with a size larger than a use size of the encapsulated immobilizing carrier; storing the carrier block in water at 15 deg.C or less or in the atmosphere with a relative humidity of 90% or more and a temperature of 15 deg.C or less until the encapsulated immobilization carrier is used; and cutting the stored carrier block into the use size of the encapsulated immobilization carrier when the encapsulated immobilization carrier is used.
摘要翻译：提供了可以稳定地储存和运输载体块的封装的固定载体的储存和生产方法，并且可以获得具有高质量稳定性的封装的固定载体。在包封的固定化载体（30）的存储方法中，将微生物包封并固定在固定材料中，直到将封装的固定化载体用于处理槽中，该方法包括：将大尺寸载体块（28）储存在水中在15℃以下或在相对湿度为90％以上且温度为15℃以下的气氛中，将载体块切割成胶囊固定化载体的使用尺寸，直到胶囊化固定载体为用过的。一种生产包封的固定化载体（30）的方法，其中将微生物包封并固定在固定材料中，该方法包括：将其中微生物与固定材料混合的液体混合物聚合成凝胶以形成载体块（28 ），其尺寸大于胶囊固定载体的使用尺寸; 将载体块储存在15℃或更低的水中或在相对湿度为90％以上且温度为15摄氏度以下的气氛中，直到使用胶囊化固定载体为止; 并且当使用封装的固定载体时，将储存的载体块切割成胶囊固定载体的使用尺寸。

95. 发明公开

KR1020060132265A 초미립 고정화 발광미생물의 제조방법 및 이를 이용한 유독물질 모니터링 방법 无效
标题翻译：微阵列固定辐射微波及其制造方法和使用有毒材料监测方法
公开(公告)号：KR1020060132265A
公开(公告)日：2006-12-21
申请号：KR1020050052512
申请日：2005-06-17
申请人： 주식회사 엔바이져코리아
发明人： 박경순
IPC分类号： C12N11/04 , C12N11/00 , C12N11/02
摘要： A luminous microorganism immobilized in microsomes, a manufacturing method thereof and a monitoring method of poisonous materials by using the same microorganism are provided to enhance sensitivity of the monitoring by reducing the size of microsomes, and improve monitoring accuracy by minimizing the errors caused by a control group. The luminous microorganism immobilized in microsomes having diameter of 0.01-0.03 mm is provided, wherein the luminous microorganism is Vibrio fischeri, and shows reduced luminescence in proportion to concentration of poisonous materials in a sample; and the immobilization material is alginic acid, agar, carrageenan or polyacrylamide. The method for manufacturing the luminous microorganism immobilized in microsomes comprises the steps of: mixing the luminous microorganism with the immobilization material, and culturing the mixture in seawater complete medium at 22-27 deg. C for 45-50 hours; suspending the recovered luminous microorganism cells with 0.5-0.8% physiological saline solution, centrifuging the cells and resuspending them with 2-3% physiological saline solution; and mixing the suspended microorganism solution with the immobilization material, adding dropwise of the mixture to calcium salt or strontium salt solution, and maintaining the solution at 0-5 deg. C for 1-3 hours to obtain gel type microbeads.
摘要翻译：提供固定在微粒体中的发光微生物，其制造方法和通过使用相同微生物的有害物质的监测方法，以通过减小微粒体的尺寸来提高监测的灵敏度，并通过最小化由控制引起的误差来提高监测精度组。提供了固定在直径为0.01-0.03mm的微粒体中的发光微生物，其中发光微生物是弧菌，并且与样品中有毒物质的浓度成比例地显示出降低的发光; 固定物为藻酸，琼脂，角叉菜胶或聚丙烯酰胺。用于制造固定在微粒体中的发光微生物的方法包括以下步骤：将发光微生物与固定化材料混合，并在22-27度的海水完全培养基中培养该混合物。 C为45-50小时; 将回收的发光微生物细胞悬浮在0.5-0.8％生理盐水溶液中，离心细胞并用2-3％生理盐水重新悬浮; 并将悬浮的微生物溶液与固定化材料混合，将混合物滴加到钙盐或锶盐溶液中，并将溶液保持在0-5℃。 C 1-3小时得到凝胶型微珠。

96. 发明公开

KR1020060043555A 고정화미생물의 제조방법, 이에 의해 제조된 고정화미생물및 그 고정화미생물을 사용한 반응 장치 无效
标题翻译：生产固定化微生物的方法，使用其制备的固定化微生物和使用其的反应装置
公开(公告)号：KR1020060043555A
公开(公告)日：2006-05-15
申请号：KR1020050019455
申请日：2005-03-09
申请人： 가부시키가이샤 히타치플랜트테크놀로지
发明人： 수미노타추오 , 아오야마코우타로우 , 이사카카주이치 , 아베나오키
IPC分类号： C12N11/02 , C12N11/06 , C12N11/08 , C12N11/04
CPC分类号： Y02E50/17 , Y02W10/15 , C12N11/08 , C02F3/34 , C02F2301/04
摘要： The invention provides a method for producing the immobilized microorganism is characterized by mixing a prepolymer having a mol. wt. of 3,500 to 20,000 with a crosslinking agent having a mol. wt. of >=71 to

97. 发明授权

KR100147891B1 알지네이트 젤로된 투과성 모세관의 제조방법 및 그 용도 失效
标题翻译：酸性凝胶渗透性毛细管柱的制造方法及其应用
公开(公告)号：KR100147891B1
公开(公告)日：1998-08-01
申请号：KR1019950008112
申请日：1995-04-07
申请人： 한국과학기술연구원
发明人： 김익환 , 박영훈 , 임동준 , 김범수 , 정용주
IPC分类号： C12N11/04 , C12N11/00
摘要： 본 발명은 알지네이트 젤로딘 투과성 모세관의 제조방법 및 그 용도에 관한 것으로서, 더욱 상세하게는 알지네이트 젤을 재료로 하여 다양한 직경을 가지는 투과성 모세관을 제조하는 방법과 동물세포를 포함한 식물, 곤충, 어류, 박테리아, 효모, 콤팡이 등의 각종 생물로부터 유래된 세포를 고농도로 배양하는데 상기 투과성 모세관을 이용하는 용도에 관한 것이다.

98. 发明授权

KR1019950012801B1 섬유질 분해효소계 제조용 진균 失效
标题翻译：真菌用于生产纤维素酶
公开(公告)号：KR1019950012801B1
公开(公告)日：1995-10-21
申请号：KR1019920010212
申请日：1992-06-12
申请人： 주식회사 엘지
发明人： 이기붕 , 김여경 , 이영하
IPC分类号： C12N11/04
摘要： The cellulase (I) from fungi Cephalosporium RYM 202 (KCTC 0042BP) (II) has kept above 80% of its hydrolysing activities at the conditions of 1) temp. of 22-53 deg.C, 2) pH 3.8-11.8, and 3) the 0.05% surfactant solutions of AOS, AS, AES, soap base or zeolite. After culturing (II) on the medium of 2% wheat bran and 0.5% sodium carbonate at pH 10.5 and 20 deg.C for 3 days, 80 unit of (I) is harvested. Alkaline (I) is useful for detergent formulation.
摘要翻译：来自真菌Cephalosporium RYM 202（KCTC 0042BP）（II）的纤维素酶（I）在1）温度条件下保持了其水解活性的80％以上。的22-53℃，2）pH 3.8-11.8，和3）0.05％的AOS，AS，AES，皂基或沸石的表面活性剂溶液。在2％麦麸和0.5％碳酸钠的培养基上，在pH 10.5和20℃培养3天后，收获80单位（I）。碱（I）可用于洗涤剂配方。

99. 发明授权

KR100056588B1 생세포 고정화계를 이용한 코오지산의 고농도 생산방법 失效
公开(公告)号：KR100056588B1
公开(公告)日：1992-11-19
申请号：KR1019900012552
申请日：1990-08-14
申请人： 주식회사 코오롱 , 한국과학기술원
发明人： 이준식 , 곽무영 , 박호진
IPC分类号： C12N11/04

100. 发明授权

KR1019910004968B1 고정화 프럭토실트랜스퍼라제의 제조법 失效
公开(公告)号：KR1019910004968B1
公开(公告)日：1991-07-20
申请号：KR1019890016483
申请日：1989-11-14
申请人： 대상 주식회사
发明人： 김민홍 , 최성수 , 인만진 , 최경호 , 한민수 , 임번삼
IPC分类号： C12N11/04
摘要： 내용 없음.

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