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2. 发明公开

KR1020070077628A 푸자리시딘 생합성 효소 및 이를 코딩하는 유전자 有权
标题翻译： FUSARICIDIN SYNTHETASE及其基因
公开(公告)号：KR1020070077628A
公开(公告)日：2007-07-27
申请号：KR1020060007304
申请日：2006-01-24
申请人： 한국생명공학연구원
发明人： 박승환 , 김지현 , 이충환 , 최수근 , 정해영 , 김성빈 , 박연경 , 김루미 , 류충민 , 박수영
IPC分类号： C12N15/52 , C12N15/10
CPC分类号： C12P17/189 , C12N9/93 , C12N15/52 , C12P21/02
摘要： A fusaricidin synthetase isolated from Paenibacillus polymyxa E681 strain is provided to increase the productivity of the fusaricidin and be usefully used for preparing a novel antibiotic. The polypeptide concerned with the synthesis of fusaricidin is described as SEQ ID : NO. 2, wherein the fusaricidin is fusaricidin A, fusaricidin B, fusaricidin C, fusaricidin D, LI-F03, LI-F04, LI-F05, LI-F07, or LI-F08. The method for preparing the fusaricidin or a derivative thereof comprises the steps of: (a) introducing a gene coding the polypeptide into an expression vector; (b) introducing the expression vector into a host cell to transform it; (c) culturing the transformant; and (d) isolating and purifying the fusaricidin or the derivative thereof from the culture material obtained from the step(c).
摘要翻译：提供从多粘类芽孢杆菌E681菌株分离的fusaricidin合成酶以提高fusaricidin的生产力并且有用地用于制备新的抗生素。涉及fusaricidin合成的多肽描述为SEQ ID NO： 2，其中所述的枯草菌素是无菌螨螨A，镰刀菌idin idin B，镰刀菌素C，镰孢菌素D，LI-F03，LI-F04，LI-F05，LI-F07或LI-F08。制备迷迭香子或其衍生物的方法包括以下步骤：（a）将编码该多肽的基因导入表达载体中; （b）将表达载体导入宿主细胞以进行转化; （c）培养转化体; 和（d）从步骤（c）获得的培养物中分离和纯化枯草精或其衍生物。

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