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    • 1. 发明公开
    • 프로테인 포스파테이스를 이용한 마이크로시스틴의비색정량법
    • 使用蛋白质磷酸酶的微量蛋白质的颜色定量
    • KR1020020005207A
    • 2002-01-17
    • KR1020000035399
    • 2000-06-26
    • 한국생명공학연구원
    • 오희목이석준윤병대김지환김희식
    • C12Q1/42
    • PURPOSE: A colorimetric quantification of microcystin using protein phosphatase is provided, thereby the amount of inorganic phosphrous can be simply and cheaply measured. CONSTITUTION: The colorimetric quantification of microcystin comprises the steps of: adding protein phosphatase and phosphorylase a as a template into a microcystin-containing sample; analyzing the amount of inorganic phosphrous(PO43-) released from the phosphorylase a, in which quantitative analysis of the amount of inorganic phosphrous is carried out by determining the optical density using a coupler, wherein the coupler contains ammonium molybdate, sulfuric acid, potassium antimonyl tartrate and ascorbic acid; and a microplate reader is used for the quantitative analysis of microcystin.
    • 目的:提供使用蛋白磷酸酶的微囊藻毒素的比色定量,从而可以简单且廉价地测量无机磷的数量。 构成:微囊藻毒素的比色定量包括以下步骤:将蛋白磷酸酶和磷酸化酶a作为模板加入到含微囊藻毒素的样品中; 分析从磷酸化酶a释放的无机磷(PO43-)的量,其中通过使用成色剂测定光密度来进行无机磷的量的定量分析,其中成色剂含有钼酸铵,硫酸,锑酸钾 酒石酸盐和抗坏血酸; 并使用酶标仪进行微囊藻毒素的定量分析。
    • 6. 发明公开
    • 신균주 캔디다 속 SY16및 이로부터 생산되는 계면활성제
    • MICROORGANISM,CANDIDA SP。 SY16(KCTC 8950P)和由其生产的表面活性剂
    • KR1020010011634A
    • 2001-02-15
    • KR1019990031104
    • 1999-07-29
    • 한국생명공학연구원
    • 윤병대김희식오희목이석준
    • C12N1/14
    • PURPOSE: A microorganism Candida sp. SY16(KCTC 8950P) and a surfactant produced from the same are provided for effectively removing the hydrophobic contaminants such as waste oil and leakage oil from environment. CONSTITUTION: The microorganism Candida sp. SY16(KCTC 8950P) producing a surfactant that is capable of removing the hydrophobic contaminants is isolated from the soil around Daejeon by screening and identifying procedures. The surfactant produced by Candida sp. SY16(KCTC 8950P) is represented by formula (I), in which R1 and R2 are the same or different and are saturated or unsaturated fatty acids having C6, C12 or C14 chain, and R3 is acetyl. The surfactant is produced by incubating the microorganism Candida sp. SY16(KCTC 8950P) in a medium containing 1.5 to 2.0 wt.% of agar, 5 to 20 wt.% of soybean oil, 0.1 to 0.5 wt.% of NH4NO3, 0.25 to 0.26 wt.% of K2HPO4, 0.01 to 0.02 wt.% of NaH2PO4, 0.04 to 0.06 wt.% of MgSO4*7H2O, 0.005 to 0.015 wt.% of CaCl2*2H2O, 0.001 to 0.003 wt.% of MnSO4*5H2O and 0.05 to 0.2 wt.% of peptone at 25 to 35 deg. C and pH 7.0 to 8.5.
    • 目的:微生物假丝酵母属 提供SY16(KCTC 8950P)和由其制备的表面活性剂,以有效去除环境中的疏油污染物如废油和泄漏油。 构成:微生物假丝酵母属 通过筛选和鉴定程序,从大田周围的土壤中分离产生能够除去疏水性污染物的表面活性剂的SY16(KCTC 8950P)。 念珠菌属 SY16(KCTC 8950P)由式(I)表示,其中R1和R2相同或不同,为具有C6,C12或C14链的饱和或不饱和脂肪酸,R3为乙酰基。 表面活性剂通过温育微生物假丝酵母属 SY16(KCTC 8950P)在含有1.5〜2.0重量%琼脂,5〜20重量%大豆油,0.1〜0.5重量%NH 4 NO 3,0.25〜0.26重量%的K 2 HPO 4,0.01〜0.02重量% ,NaH 2 PO 4%,0.04至0.06重量%的MgSO 4 * 7H 2 O,0.005至0.015重量%的CaCl 2 * 2H 2 O,0.001至0.003重量%的MnSO 4 * 5H 2 O和0.05至0.2重量%的蛋白胨,25至35 度。 C和pH 7.0至8.5。
    • 7. 发明授权
    • 프로테인 포스파테이스를 이용한 마이크로시스틴의비색정량법
    • 我们的技术研发工作团队成员
    • KR100381718B1
    • 2003-04-26
    • KR1020000035399
    • 2000-06-26
    • 한국생명공학연구원
    • 오희목이석준윤병대김지환김희식
    • C12Q1/42
    • PURPOSE: A colorimetric quantification of microcystin using protein phosphatase is provided, thereby the amount of inorganic phosphrous can be simply and cheaply measured. CONSTITUTION: The colorimetric quantification of microcystin comprises the steps of: adding protein phosphatase and phosphorylase a as a template into a microcystin-containing sample; analyzing the amount of inorganic phosphrous(PO43-) released from the phosphorylase a, in which quantitative analysis of the amount of inorganic phosphrous is carried out by determining the optical density using a coupler, wherein the coupler contains ammonium molybdate, sulfuric acid, potassium antimonyl tartrate and ascorbic acid; and a microplate reader is used for the quantitative analysis of microcystin.
    • 目的:提供使用蛋白磷酸酶的微囊藻毒素的比色定量,因此无机亚磷的量可以简单且便宜地测量。 构成:微囊藻毒素的比色定量包括以下步骤:将蛋白质磷酸酶和磷酸化酶a作为模板加入到含有微囊藻毒素的样品中; 分析从磷酸化酶a释放的无机亚磷酸(PO43-)的量,其中通过使用成色剂测定光密度来进行无机亚磷酸的量的定量分析,其中成色剂包含钼酸铵,硫酸,锑酸钾 酒石酸盐和抗坏血酸; 并使用酶标仪进行微囊藻毒素的定量分析。