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1. 发明专利

JP2012024039A Nucleic acid primer set for lamp amplification for corona virus 有权
标题翻译：用于CORONA病毒的灯泡放大的核酸准备组
公开(公告)号：JP2012024039A
公开(公告)日：2012-02-09
申请号：JP2010167491
申请日：2010-07-26
申请人： Central Institute For Experimental Animals , Toshiba Corp , 公益財団法人実験動物中央研究所 , 株式会社東芝
发明人： HORIUCHI HIDENORI , ITO KEIKO , HAYASHIMOTO NOBUTO , TAKAKURA AKIRA , ONODERA MAKOTO , HASHIMOTO KOJI , MOTOMA NOBUHIRO , NIKAIDO MASARU
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： Y02A50/451
摘要： PROBLEM TO BE SOLVED: To provide a primer set for specifically amplifying a corona virus-originated nucleic acid, and a method for detecting and/or classifying the virus.SOLUTION: There is provided the primer set, wherein the primer set is the nucleic acid primer set for LAMP amplification for specifically amplifying the corona virus-originated nucleic acid; each of FIP primer, BIP primer, F3 primer and B3 primer is at least one kind of primer that includes a polynucleotide composed of a specific sequence or a polynucleotide including a mutation in a range in which the corona virus-originated nucleic acid can be specifically amplified.
摘要翻译：要解决的问题：提供用于特异性扩增电晕病毒起源的核酸的引物组，以及用于检测和/或分类病毒的方法。提供了引物组，其中引物组是用于特异性扩增电晕病毒起源的核酸的LAMP扩增的核酸引物组; FIP引物，BIP引物，F3引物和B3引物各自是至少一种引物，其包含由特定序列组成的多核苷酸或包含在电晕病毒起源的核酸特异性范围内的突变的多核苷酸放大。版权所有（C）2012，JPO＆INPIT

2. 发明专利

JP2010011849A Nucleic acid primer kit for detecting genotype of n-acetyltransferase 2 (nat2), kit, and detection method using the primer set 有权
标题翻译：用于检测N-乙酰基转移酶2（NAT2），试剂盒和检测方法的基因组的核酸试剂盒
公开(公告)号：JP2010011849A
公开(公告)日：2010-01-21
申请号：JP2009172316
申请日：2009-07-23
申请人： Toshiba Corp , 株式会社東芝
发明人： NAKAMURA NAOKO , ITO KEIKO , HASHIMOTO KOJI , MOTOMA NOBUHIRO
IPC分类号： C12Q1/68 , C12N15/09 , G01N27/327
摘要： PROBLEM TO BE SOLVED: To provide a method for simply and inexpensively detecting a single nucleotide polymorphism of NAT2 gene in a short time, a nucleic acid primer used for the detection, and a probe for detection. SOLUTION: A nucleic acid prime set for LAMP amplification detects a genotype in a single nucleotide polymorphism G857A of a human NAT2 gene. The nucleic acid primer set comprises an FIP primer having a specific sequence, a BIP primer, an F3 primer and a B3 primer. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供一种在短时间内简单且廉价地检测NAT2基因的单核苷酸多态性的方法，用于检测的核酸引物和用于检测的探针。解决方案：用于LAMP扩增的核酸序列检测人NAT2基因的单核苷酸多态性G857A中的基因型。核酸引物组包含具有特异性序列的FIP引物，BIP引物，F3引物和B3引物。版权所有（C）2010，JPO＆INPIT

3. 发明专利

JP2010011791A Method for detecting multiple nucleic acids 审中-公开
标题翻译：检测多种核酸的方法
公开(公告)号：JP2010011791A
公开(公告)日：2010-01-21
申请号：JP2008174927
申请日：2008-07-03
申请人： Toshiba Corp , 株式会社東芝
发明人： OKADA JUN , MOTOMA NOBUHIRO
IPC分类号： C12N15/09 , C12M1/00 , C12Q1/68 , G01N33/53 , G01N37/00
CPC分类号： C12Q1/6837
摘要： PROBLEM TO BE SOLVED: To provide a detection method preventing misdetection due to garbling of specimens and contamination of the specimens, and having high safety and reliability required at a medical site.
SOLUTION: The method for detecting multiple nucleic acids includes a first step for preparing devices for detecting nucleic acid samples, a second step for preparing first to nth reagents for identifying the nucleic acid samples, a third step for adding the first to nth reagents to the first to nth nucleic acid samples respectively, a fourth step for injecting the first to nth nucleic samples to first to nth wells respectively, a fifth step for detecting the presence or absence of reactions at positive control-immobilized regions in the first to nth wells respectively, and a sixth step for detecting the presence or absence of the reactions in the detection nucleic acid probe-immobilized regions in the first to nth wells respectively.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种检测方法，其防止由于试样的混乱和样品的污染而导致的错误检测，并且在医疗部位需要高安全性和可靠性。解决方案：用于检测多个核酸的方法包括用于制备用于检测核酸样品的装置的第一步骤，用于制备用于鉴定核酸样品的第一至第n试剂的第二步骤，第一至第n 分别将第一至第n个核酸样品注射到第一至第n个第一个孔的第四个步骤，第五个步骤，用于检测在第一至第n个核酸样品的阳性对照固定化区域中存在或不存在反应分别在第1〜第n孔中检测检测核酸探针固定化区域中存在或不存在反应的第6步骤。版权所有（C）2010，JPO＆INPIT

4. 发明专利

JP2007271326A Method of manufacturing probe fixing substrate 审中-公开
标题翻译：制造探针固定基板的方法
公开(公告)号：JP2007271326A
公开(公告)日：2007-10-18
申请号：JP2006094363
申请日：2006-03-30
申请人： Toshiba Corp , 株式会社東芝
发明人： TAKAHASHI MASAYOSHI , MOTOMA NOBUHIRO , OKADA JUN , HORIUCHI HIDENORI
IPC分类号： G01N27/416 , G01N27/04 , G01N27/327 , G01N33/53 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide a method of manufacturing a probe fixing substrate capable of performing the total inspection in a manufacturing process of a probe fixing substrate so that all of the manufactured probe fixing substrates have good measuring precision. SOLUTION: The method of manufacturing a bio-molecule probe fixing substrate for detecting a bio-molecule includes a step of fixing the bio-molecule probe capable of interacting with a target bio-molecule to the electrode provided to the substrate and a step of electrochemically measuring the amount of the bio-molecule probe fixed to the electrode of the substrate to except electrode out of a predetermined range in fixing amount of the fixing amount of the bio-molecule probe. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：提供一种制造能够在探针固定基板的制造过程中进行全面检查的探针固定基板的方法，使得所制造的所有探针固定基板具有良好的测量精度。解决方案：制造用于检测生物分子的生物分子探针固定基底的方法包括将能够与靶生物分子相互作用的生物分子探针固定到提供给基底的电极的步骤和在生物分子探针的固定量的固定量的固定量中，将固定于基板的电极的生物分子探针的量进行电化学测定的步骤。版权所有（C）2008，JPO＆INPIT

5. 发明专利

JP2006262788A Reactor 有权
标题翻译：反应堆
公开(公告)号：JP2006262788A
公开(公告)日：2006-10-05
申请号：JP2005085954
申请日：2005-03-24
申请人： Toshiba Corp , Toshiba Tec Corp , 東芝テック株式会社 , 株式会社東芝
发明人： NUMATA AKIKO , HONGO SADAHITO , MOTOMA NOBUHIRO
IPC分类号： C12M1/00 , C12M1/38 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a reactor capable of preventing unevenness of temperature in its inside, when the temperature is controlled, further capable of preventing liquid spillage, and given by utilizing a closed-type cassette.
SOLUTION: This reactor is equipped with a closed-type reaction vessel in which a channel for retaining a sample and a reagent is formed by using stationary members and a flexible member. Heat transfer blocks for controlling the temperature of the closed-type reaction vessel are structured by bringing first and second contact members thereof into contact with the flexible member and the stationary members, so that a closed space is formed between the flexible member corresponding to the channel and the first contact member, when the temperature is controlled.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：为了提供一种能够防止其内部温度不均匀的反应器，当控制温度时，还能够防止液体溢出，并且通过利用封闭式盒带给出。解决方案：该反应器配备有封闭式反应容器，其中通过使用固定构件和柔性构件形成用于保持样品和试剂的通道。用于控制封闭式反应容器的温度的传热块通过使第一和第二接触构件与柔性构件和固定构件接触而构成，使得在与通道对应的柔性构件之间形成封闭空间和第一接触构件，当温度受到控制时。版权所有（C）2007，JPO＆INPIT

6. 发明专利

JP2004121044A Method for analyzing nucleic acid sequence, substrate for immobilizing probe, assay kit and probe set 有权
公开(公告)号：JP2004121044A
公开(公告)日：2004-04-22
申请号：JP2002287376
申请日：2002-09-30
申请人： Toshiba Corp , 株式会社東芝
发明人： MOTOMA NOBUHIRO , HASHIMOTO KOJI
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method for analyzing a nucleic acid sequence for detecting the nucleic acid sequence with high specificity and to provide a substrate for immobilizing a probe, an assay kit and a probe set. SOLUTION: The method for analyzing the nucleic acid sequence comprises reacting a nucleic acid in a sample with a first probe containing a first target site and a first sequence and a second probe containing a sequence shorter than the first sequence by one base and homologous except the one base of a second target site corresponding to the position of the first target site under conditions for initiating suitable hybridization. COPYRIGHT: (C)2004,JPO

7. 发明专利

JP2014060973A Multiple nucleic acid reaction tool, production method thereof and method of amplifying or detecting nucleic acid using the same 有权
标题翻译：多种核酸反应工具及其生产方法及使用其的放大或检测核酸的方法
公开(公告)号：JP2014060973A
公开(公告)日：2014-04-10
申请号：JP2012208842
申请日：2012-09-21
申请人： Toshiba Corp , 株式会社東芝
发明人： INADA YOSHIMASA , ITO KEIKO , HASHIMOTO KOJI , NIKAIDO MASARU , MOTOMA NOBUHIRO
IPC分类号： C12M1/00 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a multiple nucleic acid reaction tool capable of conducting a plurality of amplification reactions in one reaction field, a production method thereof, and a method of amplifying or detecting nucleic acid using the same.SOLUTION: A multiple nucleic acid reaction tool according to the embodiment is equipped with a support configured to support a liquid-phase reaction field, a plurality of primer immobilization regions independently arranged on at least one surface of the support which is in contact with the reaction field when the reaction field has been formed using the liquid phase, a plurality of amplification reagent immobilization regions arranged at the same location or in the vicinity of each of the plurality of primer immobilization regions, a plurality of species of primer sets immobilized in a releasable manner according to each species on the plurality of primer immobilization regions, and an amplification reagent comprising a plurality of compositions immobilized on the amplification reagent immobilization regions in a releasable manner according to each composition, configured to enable amplification reactions with each of the plurality of species of primer sets.
摘要翻译：要解决的问题：提供能够在一个反应场中进行多个扩增反应的多重核酸反应工具，其制备方法和使用其的扩增或检测核酸的方法。解决方案：多重核酸根据实施方案的反应工具装备有支撑液相反应场的支撑体，多个引物固定区域，独立地设置在与反应场接触的载体的至少一个表面上，当反应场具有使用液相形成多个扩增试剂固定化区域，多个扩增试剂固定化区域配置在多个引物固定化区域中的每一个的相同位置或附近，多种引物组，根据各种物质以可释放的方式固定多个引物固定区，以及包含多个引物固定区的扩增试剂根据每个组合物以可释放的方式固定在扩增试剂固定区上的孔，其配置为能够与多种引物组中的每一种进行扩增反应。

8. 发明专利

JP2013198419A Nucleic acid detection device 有权
标题翻译：核酸检测装置
公开(公告)号：JP2013198419A
公开(公告)日：2013-10-03
申请号：JP2012067946
申请日：2012-03-23
申请人： Toshiba Corp , 株式会社東芝
发明人： OKADA JUN , NIKAIDO MASARU , MOTOMA NOBUHIRO , HIROZAWA DAIJI , YAMAMOTO KEIICHI , KUWABARA TETSUYA , TAKASE MADOKA
IPC分类号： C12M1/34 , C12N15/09 , G01N27/327
摘要： PROBLEM TO BE SOLVED: To provide a nucleic acid detection device for reducing the inhibition of an amplification reaction of a nucleic acid.SOLUTION: This nucleic acid detection device includes a substrate, a sensor part, wiring, and a protection part. The sensor part is for nucleic acid detection, formed on the substrate. The wiring is formed on the substrate and connected with the sensor. The protection membrane is formed on the substrate. The nucleic acid detection device performs detection of a nucleic acid amplification product by the sensor part after a nucleic acid amplification reaction is performed in a chamber for the sensor part and a nucleic acid sample to react. The protection membrane is provided with one or more openings to expose a lower part including a part of the substrate in a liquid contact region of the nucleic acid sample on the substrate.
摘要翻译：要解决的问题：提供一种用于降低核酸扩增反应抑制的核酸检测装置。解决方案：该核酸检测装置包括基板，传感器部件，布线和保护部件。传感器部分用于在基底上形成的核酸检测。布线形成在基板上并与传感器连接。保护膜形成在基板上。核酸检测装置在用于传感器部分的室和核酸样品进行核酸扩增反应之后，通过传感器部分进行核酸扩增产物的检测。保护膜设置有一个或多个开口，以在衬底上的核酸样品的液体接触区域中露出包括衬底的一部分的下部。

9. 发明专利

JP2013066463A Multi-nucleic acid reaction tool and detection method using same 有权
标题翻译：多核酸反应工具及使用相同的检测方法
公开(公告)号：JP2013066463A
公开(公告)日：2013-04-18
申请号：JP2012197912
申请日：2012-09-07
申请人： Toshiba Corp , 株式会社東芝
发明人： TAKAHASHI MASAYOSHI , TAKASE MADOKA , OKADA JUN , HASHIMOTO KOJI , NIKAIDO MASARU , MOTOMA NOBUHIRO , WATANABE KENICHI , TSUJI KOICHI
IPC分类号： C12M1/34 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a multi-nucleic reaction tool capable of subjecting a plurality of different target sequences to an amplification reaction simultaneously and independently using a plurality of different primer sets in one reaction field, and independently determining a presence/absence or the amount of the target sequences for each of amplified products of the primer sets, and to provide a method for detecting a target nucleic acid using the same.SOLUTION: A multi-nucleic acid reaction tool of an embodiment is equipped with a support, a plurality of different primer sets, and probe nucleic acids comprising a complementary sequence of a target sequence. The support is configured to support a liquid-phase reaction field. On at least one surface of the support which is in contact with the reaction field when the reaction field is formed using the liquid phase, the plurality of different primer sets are fixed in a releasable manner to mutually independent primer immobilization regions for each type of primer. Each of the plurality of different primer sets is configured to amplify the respective corresponding target sequence. The probe nucleic acids are fixed to the same positions as the primer immobilization regions or to probe immobilization regions in proximity thereto, and hybridized signals are independently detected.
摘要翻译：待解决的问题：提供能够在一个反应场中使用多个不同引物组同时且独立地使多个不同靶序列进行扩增反应的多核酸反应工具，并且独立地确定存在/ 不存在或引物组的每个扩增产物的靶序列的量，以及提供使用其的靶核酸的检测方法。解决方案：实施方案的多核酸反应工具装备有载体，多个不同的引物组和包含靶序列的互补序列的探针核酸。支持体配置成支持液相反应场。在使用液相形成反应场时，在与反应场接触的载体的至少一个表面上，将多种不同的引物组以可分离的方式固定到每种引物的相互独立的引物固定区。多个不同引物组中的每一个被配置为放大相应的对应序列。将探针核酸固定至与引物固定区域相同的位置或探测其附近的固定区域，并独立地检测杂交信号。版权所有（C）2013，JPO＆INPIT

10. 发明专利

JP2013051956A Method for analyzing nucleic acid 审中-公开
标题翻译：分析核酸的方法
公开(公告)号：JP2013051956A
公开(公告)日：2013-03-21
申请号：JP2012166940
申请日：2012-07-27
申请人： Toshiba Corp , 株式会社東芝
发明人： NAKAMURA NAOKO , HASHIMOTO KOJI , HASHIMOTO MICHIE , MOTOMA NOBUHIRO , NIKAIDO MASARU
IPC分类号： C12Q1/68 , C12N15/09 , G01N33/483
CPC分类号： C12Q1/6837 , C12Q1/6853 , C12Q2525/301
摘要： PROBLEM TO BE SOLVED: To provide a method for analyzing nucleic acid accurately and easily in a short time.SOLUTION: The method for analyzing nucleic acid on a specimen includes performing an amplification reaction of the specimen using a first primer and a second primer, reacting an amplification reaction product with a nucleic acid probe, measuring the presence/absence and/or amount of hybridization, and determining the presence/absence and/or abundance of target nucleic acid in the specimen. An amplification product produced using the first primer and/or the second primer forms a stem-loop structure. A nucleic acid analysis on the specimen is made by detecting the presence/absence and/or abundance of hybridization between the amplification product and the nucleic acid probe complementary to the sequence of the loop structure.
摘要翻译：要解决的问题：提供在短时间内准确且容易地分析核酸的方法。解决方案：用于分析样品上的核酸的方法包括使用第一引物和第二引物进行样品的扩增反应，使扩增反应产物与核酸探针反应，测量存在/不存在和/或杂交量，并确定样品中靶核酸的存在/不存在和/或丰度。使用第一引物和/或第二引物产生的扩增产物形成茎 - 环结构。通过检测扩增产物和与环结构序列互补的核酸探针之间的杂交的存在/不存在和/或丰度来进行样品的核酸分析。版权所有（C）2013，JPO＆INPIT

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