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1. 发明专利

JP2010189662A Method for stabilizing leuco pigment 审中-公开
标题翻译：稳定色素的方法
公开(公告)号：JP2010189662A
公开(公告)日：2010-09-02
申请号：JP2010111093
申请日：2010-05-13
申请人： Asahi Kasei Pharma Kk , 旭化成ファーマ株式会社
发明人： UEDA SHIGERU , MATSUOKA TAKESHI , SUGA AKIHIKO
IPC分类号： C09B67/00 , C09B11/14 , C09B21/00 , C09B67/44 , G01N33/00
摘要： PROBLEM TO BE SOLVED: To provide a method for stabilizing a leuco pigment, capable of being stored for a long term as liquid, to provide a method for reducing nonspecific color development during developing the reaction of the leuco pigment, and to provide a reagent composition stable as a liquid using the method. SOLUTION: The facts that, by coexisting the leuco pigment with a specific reductant, natural color development is suppressed and stability as liquid is much improved, and, by coexisting another pigment with the reaction liquid, which pigment have absorption spectrum giving no effect on the measuring wave length of the leuco pigment in developing reaction using the leuco type pigment and with hydrogen peroxide and does not react with hydrogen peroxide, the nonspecific color development is suppressed, and the reagent blank value is decreased, are found and applied to the reagent for analysis. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供一种能够长期作为液体储存的无色颜料的稳定化方法，以提供在显色无色颜料的反应期间减少非特异性显色的方法，并提供使用该方法作为液体稳定的试剂组合物。解决方案：通过将无色颜料与特定的还原剂共存，自然色发展被抑制，液体的稳定性大大提高，并且通过将另一种颜料与反应液共存，该颜料具有不吸收光谱对于使用无色型颜料和过氧化氢的显影反应中的无色颜料的测定波长的影响，不与过氧化氢反应，可以抑制非特异性显色，降低试剂空白值，并应用于用于分析的试剂。版权所有（C）2010，JPO＆INPIT

2. 发明专利

JP2008201968A Method of stabilizing leuco pigment 有权
标题翻译：稳定色素的方法
公开(公告)号：JP2008201968A
公开(公告)日：2008-09-04
申请号：JP2007041551
申请日：2007-02-22
申请人： Asahi Kasei Pharma Kk , 旭化成ファーマ株式会社
发明人： UEDA SHIGERU , MATSUOKA TAKESHI , SUGA AKIHIKO
IPC分类号： C09B67/44 , C09B11/00 , C09B21/00 , C12Q1/26 , C12Q1/28 , C12Q1/37 , G01N33/50 , G01N33/52 , G01N33/68 , G01N33/72
CPC分类号： C12Q1/28 , C09B67/0077
摘要： PROBLEM TO BE SOLVED: To provide a method of stabilizing a leuco pigment, capable of being stored for a long term as liquid, to provide a method of reducing nonspecific color development during developing the reaction of the leuco pigment, and to provide a reagent composition stable as a liquid using the method. SOLUTION: The facts that, by coexisting the leuco pigment with a specific reductant, natural color development is suppressed and stability as liquid is much improved, and, by coexisting another pigment with the reaction liquid, which pigment have absorption spectrum giving no effect on the measuring wave length of the leuco pigment in developing reaction using the leuco type pigment and with hydrogen peroxide and does not react with hydrogen peroxide, the nonspecific color development is suppressed, and the reagent blank value is decreased, are found and applied to the reagent for analysis. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：提供一种能够长期储存作为液体的无色颜料的稳定化方法，以提供在显色无色颜料的反应期间减少非特异性显色的方法，并提供使用该方法作为液体稳定的试剂组合物。解决方案：通过将无色颜料与特定的还原剂共存，自然色发展被抑制，液体的稳定性大大提高，并且通过将另一种颜料与反应液共存，该颜料具有不吸收光谱对于使用无色型颜料和过氧化氢的显影反应中的无色颜料的测定波长的影响，不与过氧化氢反应，可以抑制非特异性显色，降低试剂空白值，并应用于用于分析的试剂。版权所有（C）2008，JPO＆INPIT

3. 发明专利

JP2011120599A Method for stabilizing leuco pigment 有权
标题翻译：稳定色素的方法
公开(公告)号：JP2011120599A
公开(公告)日：2011-06-23
申请号：JP2011014758
申请日：2011-01-27
申请人： Asahi Kasei Pharma Kk , 旭化成ファーマ株式会社
发明人： UEDA SHIGERU , MATSUOKA TAKESHI , SUGA AKIHIKO
IPC分类号： C12Q1/28 , C09B11/00 , C09B11/12 , C09B21/00 , C12Q1/26 , C12Q1/37
摘要： PROBLEM TO BE SOLVED: To provide a method for stabilizing a leuco pigment which is liquified and preservable for a long time and for reducing non-specific color development of the leuco pigment at the time of reaction, and to provide the liquified stable reagent composition by the use of the method. SOLUTION: Natural color development is suppressed and stability in solution is markedly improved by making a leuco pigment co-exist with a specific reducing agent. At the time of color development reaction by the use of the leuco pigment with hydrogen peroxide, if another pigment which has absorption spectrum not affecting the wavelength of measuring the leuco pigment and which does not react with hydrogen peroxide co-exist in the reaction solution, non-specific color development is suppressed and the reagent blank value is decreased. These facts are found and applied to the reagent for analysis. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供一种稳定长时间液化和保存的无色颜料的方法，并且用于在反应时减少无色颜料的非特异性显色，并提供液化稳定的试剂组合使用方法。解决方案：通过使无色颜料与特定还原剂共存，抑制了自然色发展和溶液中的稳定性显着提高。在通过使用无色颜料与过氧化氢进行显色反应的时候，如果具有不影响测定无色颜料的波长并且不与过氧化氢反应的吸收光谱的另一种颜料在反应溶液中共存，非特异性显色被抑制，试剂空白值降低。这些事实被发现并应用于试剂进行分析。版权所有（C）2011，JPO＆INPIT

4. 发明专利

JP2013158291A Measurement method for mevalonic acid using enzyme cycling reaction and calibration sample used for the same 有权
标题翻译：使用酶循环反应的测定方法和使用其的校准样品
公开(公告)号：JP2013158291A
公开(公告)日：2013-08-19
申请号：JP2012022383
申请日：2012-02-03
申请人： Asahi Kasei Pharma Kk , 旭化成ファーマ株式会社
发明人： MATSUOKA TAKESHI , UEDA SHIGERU
IPC分类号： C12Q1/26 , C07C59/105 , C12Q1/48
摘要： PROBLEM TO BE SOLVED: To provide a measurement method of mevalonic acid, including the steps of removing mevalonic acid in a test solution by pretreatment and subjecting the solution to an enzyme cycling reaction, capable of measuring the quantity of mevalonic acid in a high accuracy.SOLUTION: There is provided a measurement method for measuring the quantity of mevalonic acid in a test solution by subjecting the solution to an enzyme cycling reaction using hydroxy-methylglutaryl coenzyme A reductase, and a calibration sample is a solution containing 3-hydroxy methylglutaryl coenzyme A.
摘要翻译：要解决的问题：提供甲羟戊酸的测定方法，包括以下步骤：通过预处理除去试验溶液中的甲羟酸，并使溶液进行能够高精度地测量甲羟酸的量的酶循环反应。解决方案：提供了通过使用羟基 - 甲基戊二酰辅酶A还原酶对溶液进行酶循环反应来测量试验溶液中的甲羟酸的量的测量方法，并且校准样品是含有3-羟基甲基戊二酰辅酶A 。

5. 发明专利

JP2012210179A Method for measuring ether type phospholipid 审中-公开
标题翻译：测量其他类型磷脂的方法
公开(公告)号：JP2012210179A
公开(公告)日：2012-11-01
申请号：JP2011077355
申请日：2011-03-31
申请人： Asahi Kasei Pharma Kk , 旭化成ファーマ株式会社
发明人： SAKASEGAWA SHINICHI , UEDA SHIGERU
IPC分类号： C12Q1/44 , C12Q1/26 , C12Q1/42 , G01N33/92
摘要： PROBLEM TO BE SOLVED: To provide a method for measuring an ether type phospholipid in a sample and a measuring composition.SOLUTION: There is provided a method for measuring cholinergic ether type phospholipids in a sample including a process of decomposing a cholinergic lysoether type phospholipid into choline and a lysoether type phosphatidic acid by using an enzyme B. There is also provided a method for measuring an ethanolamine ether type phospholipids in a sample including a process of decomposing an ethanolamine lysoether type phospholipid in a sample into an ethanolamine and the lysoether type phosphatidic acid by using an enzyme B'.
摘要翻译：待解决的问题：提供一种测定样品中的醚型磷脂的方法和测定组合物。提供了一种在样品中测量胆碱能醚型磷脂的方法，包括通过使用酶B将胆碱能溶血肽型磷脂分解成胆碱和溶血肽型磷脂酸的方法。还提供了一种用于在样品中测量乙醇胺醚型磷脂，其包括通过使用酶B'将样品中的乙醇胺溶血醚型磷脂分解成乙醇胺和溶血醚型磷脂酸的方法。版权所有（C）2013，JPO＆INPIT

6. 发明专利

JP2012024014A Method for measuring choline in whole blood 审中-公开
标题翻译：用于测量全血中胆固醇的方法
公开(公告)号：JP2012024014A
公开(公告)日：2012-02-09
申请号：JP2010165457
申请日：2010-07-23
申请人： Asahi Kasei Pharma Kk , Kyushu Univ , 国立大学法人九州大学 , 旭化成ファーマ株式会社
发明人： UEDA SHIGERU , SAKASEGAWA SHINICHI , YUMURA AKIYO , SOTOZONO EISAKU , OSAWA SUSUMU
IPC分类号： C12Q1/26 , C12Q1/28
摘要： PROBLEM TO BE SOLVED: To provide a method for measuring choline in full blood and a composition for measurement.SOLUTION: The method for measuring the choline in full blood using a choline oxidase, peroxidase and oxidative coloring agent includes a step of coexisting and reacting the hemolyzed full blood, a potassium iodate, sodium iodate, hydroxylamine or salt thereof in a solution.
摘要翻译：要解决的问题：提供一种测量全血中胆碱的方法和用于测量的组合物。解决方案：使用胆碱氧化酶，过氧化物酶和氧化着色剂测量全血中的胆碱的方法包括将溶血的全血，碘酸钾，碘酸钠，羟胺或其盐在溶液中共存并使其反应的步骤。版权所有（C）2012，JPO＆INPIT

7. 发明专利

JP2006064602A Oxygen indicator 审中-公开
标题翻译：氧气指示器
公开(公告)号：JP2006064602A
公开(公告)日：2006-03-09
申请号：JP2004249437
申请日：2004-08-30
申请人： Asahi Kasei Life & Living Corp , Asahi Kasei Pharma Kk , 旭化成ファーマ株式会社 , 旭化成ライフ&リビング株式会社
发明人： UEDA ARITAKA , SAKURAI KAZUAKI , UEDA SHIGERU
IPC分类号： G01N31/00 , C12Q1/26 , G01N21/78 , G01N31/22
摘要： PROBLEM TO BE SOLVED: To provide an oxygen indicator using enzyme clearly discriminating the presence of oxygen more stably.
SOLUTION: The oxygen indicator using enzyme is composed of an oxygen responsive solution (which may contains a pH buffer agent and a reducing agent) containing a color forming substrate and oxidation-reduction enzyme using a reaction wherein a substrate changes in light absorbing spectrum trough the catalytic action of enzyme in the presence of oxygen. The pH of the oxygen responsive solution is within a specific range and the enzymatic activity M of the oxygen responsive solution before the detection of oxygen is 0.1 U/ml or above.
COPYRIGHT: (C)2006,JPO&NCIPI

8. 发明专利

JP2006061067A Oxygen indicator 审中-公开
标题翻译：氧气指示器
公开(公告)号：JP2006061067A
公开(公告)日：2006-03-09
申请号：JP2004247112
申请日：2004-08-26
申请人： Asahi Kasei Life & Living Corp , Asahi Kasei Pharma Kk , 旭化成ファーマ株式会社 , 旭化成ライフ&リビング株式会社
发明人： SAKURAI KAZUAKI , UEDA SHIGERU
IPC分类号： C12Q1/26 , C12M1/34 , C12M1/40
摘要： PROBLEM TO BE SOLVED: To provide an oxygen indicator stably detecting the presence or absence of the oxygen over a long period with high sensitivity. SOLUTION: The oxygen indicator has a structure in which an oxygen-sensitive solution containing at least an oxidoreductase and a color developing substrate is covered with an oxygen-permeable film. The oxygen indicator is characterized in that the ratio (A/B) of the color development rate (A) of the oxygen-sensitive solution to the oxygen permeation rate (B) of the oxygen-permeable film is ≥0.02 to ≤30. COPYRIGHT: (C)2006,JPO&NCIPI

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