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1. 发明专利

JP2005204501A Method for adjusting circadian rhythm 审中-公开
标题翻译：调整CIRCADIAN RHYTHM的方法
公开(公告)号：JP2005204501A
公开(公告)日：2005-08-04
申请号：JP2002023573
申请日：2002-01-31
申请人： Kenichi Honma , Yukio Kato , 幸夫加藤 , 研一本間
发明人： HONMA SATO , NOSHIRO MITSUHIDE , KAWAMOTO TAKESHI , FUJIMOTO KATSUMI , KATO YUKIO , HONMA KENICHI
IPC分类号： C12N15/09 , A61K38/00 , A61K48/00 , A61P25/00 , A61P43/00 , C07K14/47
CPC分类号： C07K14/4702 , A61K38/00 , A61K48/00
摘要： PROBLEM TO BE SOLVED: To provide a new method for controlling circadian with DEC1 and DEC2 which are basic helix-loop-helix type transcription factors in suprachiasmatic nucleus (SCN) and liver. SOLUTION: This method for controlling transcriptive activity induced with CLOCK and BMAL1 through E box enhancer is characterized by using DEC1 and DEC2 which are basic helix-loop-helix type transcription factors. The method for controlling a promoting action with DBP on the adjustment of the circadian rhythm of cholesterol 7α-hydroxylase, sterol 12α-hydroxylase, and lanosterol 14-demethylation enzyme in rat liver is characterized by using basic helix-loop-helix transcription factors DEC2/SHARP1/BHLHB3. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种控制昼夜节律的新方法，DEC1和DEC2是视交叉上核（SCN）和肝脏中的基本螺旋 - 环 - 螺旋型转录因子。解决方案：用于控制CLOCK和BMAL1通过E盒增强子诱导的转录活性的方法的特征在于使用作为碱性螺旋 - 环 - 螺旋型转录因子的DEC1和DEC2。控制DBP对大鼠肝脏中胆固醇7α-羟化酶，甾醇12α-羟化酶和羊毛甾醇14脱甲基酶的昼夜节律调节的促进作用的方法的特征在于使用基本的螺旋 - 环 - 螺旋转录因子DEC2 / SHARP1 / BHLHB3。版权所有（C）2005，JPO＆NCIPI

2. 发明专利

JP2004089095A Apparatus and method for automatically culturing cellular tissue 审中-公开
公开(公告)号：JP2004089095A
公开(公告)日：2004-03-25
申请号：JP2002256199
申请日：2002-08-30
申请人： Japan Science & Technology Corp , Yukio Kato , Marubishi Baioenji:Kk , Koichiro Tsuji , 加藤幸夫 , 株式会社丸菱バイオエンジ , 科学技術振興事業団 , 辻紘一郎
发明人： YAMAGATA TOSHIHIKO , KATO YUKIO , TSUJI KOICHIRO
IPC分类号： C12M3/00 , C12N5/07 , C12N5/071 , C12N5/06
CPC分类号： C12M41/48
摘要： PROBLEM TO BE SOLVED: To provide an apparatus for automatically producing cellular tissue safely and surely at low cost. SOLUTION: The apparatus for automatically culturing cellular tissue comprises a refrigerator, an automatically weighing device, a tube, a seed cell culturing vessel, a main cell culturing vessel, a swinging or shaking device, a culturing vessel-tilting device, a collecting device, and a control panel, wherein the refrigerator functions to refrigerate a medium container and a chemical agent container, the automatically weighing device includes a weighing tube equipped with a level sensor doubling as a liquid feed pipe functioning to automatically weigh a given amount of a medium or chemical agent, and the tube functions to feed the culturing vessel with an automatically weighed medium and chemical agent. Thus, both of the medium and chemical agent can be automatically weighed easily, surely and efficiently, effecting automatic cell production safely at low cost, furthermore, medium exchange at the culture vessel, disposal of used supernatant to a drain, and feed of both of the medium and chemical agent, etc. can all be carried out mechanically and automatically through the tube provided with an automatic pinch valve so as to open/close as necessary. COPYRIGHT: (C)2004,JPO

3. 发明专利

JP2005027579A Method for identifying/isolating mesenchymal stem cell by using fraction marker 有权
$Method for identifying/isolating mesenchymal stem cell by using fraction marker$
标题翻译：使用分类标记识别/分离中胚层干细胞的方法
公开(公告)号：JP2005027579A
公开(公告)日：2005-02-03
申请号：JP2003271649
申请日：2003-07-07
申请人： Japan Science & Technology Agency , Yukio Kato , Two Cells Co Ltd , 幸夫加藤 , 株式会社ツーセル , 独立行政法人科学技術振興機構
发明人： KATO YUKIO , TSUJI KOICHIRO , HARA MAIKO
IPC分类号： G01N33/48 , C12N5/07 , C12N5/074 , C12N5/0775 , C12N15/09 , C12Q1/68 , G01N33/50 , G01N33/53 , G01N33/566 , G01N33/577 , G01N33/68 , G01N37/00 , C12N5/06
摘要： PROBLEM TO BE SOLVED: To provide a method for effectively identifying and isolating a mesenchymal stem cell and a fibroblast the distinction of which is difficult because of resembled shapes by using a gene marker and/or a protein marker. SOLUTION: The mesenchymal stem cell and the fibroblast are identified/isolated by using a gene of a vitamin D receptor or a protein thereof, or a vitamin D receptor-associated gene such as osteocalcin and osteopontin, or a protein thereof as a fraction marker of the mesenchymal stem cell and the fibroblast, and detecting the gene and the expression of the protein in a subject cell. The identification/isolation of the mesenchymal stem cell is effectively carried out by detecting the expression of an activated gene because the expression of the target gene of the vitamin D receptor is activated in the mesenchymal stem cell by the addition of a material for activating the expression of the gene such as 1,25-dihydroxyvitamine D. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种有效鉴定和分离间充质干细胞和成纤维细胞的方法，其区别由于使用基因标记和/或蛋白质标记的类似形状而难以进行。解决方案：通过使用维生素D受体或其蛋白质的基因或维生素D受体相关基因如骨钙蛋白和骨桥蛋白或其蛋白质来鉴定/分离间充质干细胞和成纤维细胞作为间充质干细胞和成纤维细胞的分数标记，并检测受试细胞中蛋白质的基因和表达。通过检测活化基因的表达来有效地进行间充质干细胞的鉴定/分离，因为维生素D受体的靶基因的表达通过添加用于激活表达的材料在间充质干细胞中活化的基因如1,25-二羟基维生素D.版权所有（C）2005，JPO＆NCIPI

4. 发明专利

JP2004290189A Marker for detecting mesenchymal stem cell detecting marker and method for discriminating the mesenchymal stem cell by using the marker 审中-公开
标题翻译：检测MESENCHYMAL STEM CELL检测标记的标记和使用标记鉴别MESENCHYMAL STEM细胞的方法
公开(公告)号：JP2004290189A
公开(公告)日：2004-10-21
申请号：JP2004067037
申请日：2004-03-10
申请人： Japan Science & Technology Agency , Yukio Kato , Two Cells Co Ltd , 幸夫加藤 , 株式会社ツーセル , 独立行政法人科学技術振興機構
发明人： KATO YUKIO , TSUJI KOICHIRO , KOIKE CHIKA
IPC分类号： G01N33/53 , C07K14/47 , C07K16/18 , C12N20060101 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/113 , C12P21/08 , C12Q1/02 , C12Q1/68 , G01N37/00
CPC分类号： C12N15/1138 , C07K14/47 , C12Q1/6881 , C12Q2600/158
摘要： PROBLEM TO BE SOLVED: To provide a marker for detecting, separating, and/or discriminating a mesenchymal stem cell, and to provide a method for detecting, separating, and/or discriminating the mesenchymal stem cell, by using the marker. SOLUTION: A gene given in sequence table is specifically expressed in the mesenchymal stem cell. The method for discriminating the mesenchymal stem cell comprises using the gene as the marker for detecting the mesenchymal stem cell. Further, a probe for detecting a mesenchymal stem cell marker gene, a primer for polymerase chain reaction used for amplifying the gene of a specimen cell in a case of detecting the mesenchymal stem cell gene marker, a polypeptide marker for detecting the mesenchymal stem cell comprising a polypeptide which the mesenchymal stem cell marker gene expresses, an antibody used for detecting the peptide marker and specifically combining with the polypeptide marker, and the method for discriminating and separating the mesenchymal stem cell which comprises using the probe for detecting the mesenchymal stem cell marker gene and the antibody specifically combining with the polypeptide marker are provided, respectively. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供用于检测，分离和/或区分间充质干细胞的标记物，并提供通过使用标记物来检测，分离和/或鉴别间充质干细胞的方法。解决方案：序列表中给出的基因在间充质干细胞中特异性表达。用于鉴别间充质干细胞的方法包括使用该基因作为检测间充质干细胞的标志物。此外，在检测间充质干细胞基因标记的情况下，用于检测间充质干细胞标记基因的探针，用于扩增样本细胞基因的聚合酶链反应的引物，用于检测间充质干细胞的多肽标记物，包括间充质干细胞标记基因表达的多肽，用于检测肽标记并与多肽标记特异性结合的抗体，以及鉴别和分离间充质干细胞的方法，其包括使用该探针检测间充质干细胞标记分别提供与多肽标记物特异性结合的抗体基因。版权所有（C）2005，JPO＆NCIPI

5. 发明专利

JP2003052365A Separation of mesenchymal stem cell from mammalian animal and method for using the same 审中-公开
标题翻译：从马马里动物分离骨髓间充质干细胞及其使用方法
公开(公告)号：JP2003052365A
公开(公告)日：2003-02-25
申请号：JP2001249653
申请日：2001-08-20
申请人： Japan Science & Technology Corp , Yukio Kato , 幸夫加藤 , 科学技術振興事業団
发明人： KATO YUKIO , TSUTSUMI SHINICHI , MIYAZAKI KAZUKO , HARA MAIKO , KAWAGUCHI HIROYUKI , KURIHARA HIDEMI
IPC分类号： A61L27/00 , C12N5/07 , C12N5/0775 , C12N5/06
摘要： PROBLEM TO BE SOLVED: To provide a method for separating and collecting mesenchymal stem cells to utilize them for the regenerative treatment of a tissue, etc., and for the preparation of mammalian cells used for the regeneration and/or restoration of the tissue, safe for a collected parent body and also easy for collection, and also a practical method for preparing cells for transplant by using the collected mesenchymal mast cells. SOLUTION: This method for collecting mesenchymal stem cells capable of collecting the cells by using a method for separating and collecting the cells from an oral cavity tissue by a simple operation of requiring a minimum exfoliation and incision of the skin and muscle, not imposing a burden to the collected parent body, safely and simply collecting the necessary amount of the cells maintaining an excellent differentiation potency. Further, the use of the mesenchymal stem cells is provided by culturing and differentiation-inducing the separated and collected mesenchymal stem cells to prepare cells used for the regeneration and/or restoration of a tissue having high differentiation potency, and transplanting the prepared cells to the defective part and/or part for restoration such as the bone, cartilage, periodontal tissue, etc., to regenerate and/or restore the tissues.
摘要翻译：要解决的问题：提供分离和收集间充质干细胞以利用它们用于组织等的再生治疗，以及用于制备用于组织再生和/或恢复的哺乳动物细胞的方法，安全用于收集的亲体，也易于收集，也是通过使用收集的间充质细胞制备用于移植的细胞的实用方法。解决方案：这种收集间充质干细胞的方法是通过使用从口腔组织中分离和收集细胞的方法来收集细胞，通过简单的操作需要皮肤和肌肉的最小剥落和切口，而不是施加负担到收集的亲本身体，安全和简单地收集必要量的细胞维持优异的分化能力。此外，通过培养和分化诱导分离和收集的间充质干细胞来制备间充质干细胞的用途，以制备用于再生和/或恢复具有高分化能力的组织的细胞，并将制备的细胞移植到有缺陷的部分和/或诸如骨，软骨，牙周组织等的修复部分，以再生和/或恢复组织。

6. 发明专利

JP2003052360A Method for culturing mesenchymal stem cell using extracellular substrate of basement membrane cell 审中-公开
标题翻译：使用基底膜细胞细胞基质培养细胞干细胞的方法
公开(公告)号：JP2003052360A
公开(公告)日：2003-02-25
申请号：JP2001249652
申请日：2001-08-20
申请人： Japan Science & Technology Corp , Yukio Kato , 幸夫加藤 , 科学技術振興事業団
发明人： KATO YUKIO , TSUTSUMI SHINICHI , MIYAZAKI KAZUKO , HARA MAIKO
IPC分类号： A61L27/00 , C12N5/07 , C12N5/0775 , C12N5/06
摘要： PROBLEM TO BE SOLVED: To provide a new method for culturing a mesenchymal stem cell capable of obtaining extremely larger amount of the mesenchymal stem cell than a conventional method under practical conditions aiming the use of the mesenchymal stem cell for a regenerative care of a tissue. SOLUTION: It was found that, by culturing a mesenchymal stem cell in the presence of an extracellular substrate of basement membrane cells, for example, by culturing a mesenchymal stem cell on a culture dish coated with the extracellular substrate of basement membrane cells, the mesenchymal stem cell grows at an extremely high speed, it can keep a differential ability to an osteoblast, a chondrocyte or a fat cell, it can be effectively grown even with a serum of a low concentration, it can be cultured by using human serum, and further, excellent differentiation-inducing culturing is possible. Further, even in the case where culturing is carried out in a lower concentration of serum, growth ability and differential ability can be kept by adding various kinds of additives to the culture medium.
摘要翻译：要解决的问题：提供一种培养能够获得极大量的间充质干细胞的间充质干细胞的新方法，其目的在于使用间充质干细胞进行组织的再生保护的实际条件下的常规方法。解决方案：通过在基底膜细胞的细胞外基质的存在下培养间充质干细胞，例如通过在包被基底膜细胞的细胞外基质的培养皿上培养间充质干细胞，发现间充质干细胞干细胞以非常高的速度生长，可以保持成骨细胞，软骨细胞或脂肪细胞的不同能力，即使用低浓度的血清也可以有效地生长，可以通过使用人血清进行培养，此外，优异的分化诱导培养是可能的。此外，即使在较低浓度的血清进行培养的情况下，也可以通过向培养基中添加各种添加剂来保持生长能力和差异能力。

7. 发明专利

JPS6133294A Filter 失效
标题翻译：过滤
公开(公告)号：JPS6133294A
公开(公告)日：1986-02-17
申请号：JP15263884
申请日：1984-07-23
申请人： Yukio Kato
发明人： KATO YUKIO
IPC分类号： C02F3/06
CPC分类号： Y02W10/15
摘要： PURPOSE: To prevent the deterioration in function of a filter by providing perpendicularly a perforated plate or a corrugated perforated plate parallel to a filter medium in the front face of inflow surface of the filter medium layer and providing the space for accumulating activated sludge.
CONSTITUTION: One end of the inside of vessel of a main body 1 is partitioned at right angles for the flow direction by providing a perforated plate 4 perpendicularly to provide an inflow chamber A between the plate 4 and the wall surface. The part between the plate 4 and the wall surface of the other end is partitioned by providing perpendicularly the perforated plates 5, 5' parallel to the plate 4 and the space layers D, C are left back and forth and a filter medium layer B is formed by packing a filter medium 6 and a perforated plate 4' is perpendicularly provided in the space layer D to provide a flow passage space A'. An inflow pipe 2 communicating with a circulation pump is perpendicularly provided in the inflow chamber A and an inflow port 8 is opened to the part close to the bottom surface. The space layer C is used as a discharge chamber by providing a discharge pipe 3 therein and the space layer D is used as the space for accumulating the activated sludge. Water is flowed into the inflow chamber A through an inflow port 8 of inflow pipe 2 and passed through the filter medium layer B in the lateral direction and returned through the discharge port 3.
COPYRIGHT: (C)1986,JPO&Japio
摘要翻译：目的：通过在过滤介质层的流入表面的前表面垂直提供平行于过滤介质的多孔板或波纹多孔板来防止过滤器功能的恶化，并提供积聚活性污泥的空间。构成：通过垂直设置多孔板4，在板4和壁面之间设置流入室A，主体1的容器内部的一端通过垂直设置多个孔板4而沿流动方向成直角分隔。板4与另一端的壁面之间的部分通过垂直地提供平行于板4的多孔板5,5'分开，并且空间层D，C来回前后，过滤介质层B为通过将过滤介质6和多孔板4'包装而形成的空间层D垂直地设置在空间层D中，以形成流路空间A'。在流入室A中垂直设置与循环泵连通的流入管2，并且向靠近底面的部分开口流入口8。空间层C通过在其中设置排出管3而用作排出室，空间层D用作积聚活性污泥的空间。水通过流入管2的流入口8流入流入室A，横向通过过滤介质层B，通过排出口3返回。

8. 发明专利

JP2006000059A Method for promoting proliferation and differentiation of animal cell by using extracellular substrate 审中-公开
标题翻译：通过使用细胞基质促进动物细胞增殖和分化的方法
公开(公告)号：JP2006000059A
公开(公告)日：2006-01-05
申请号：JP2004180338
申请日：2004-06-17
申请人： Yukio Kato , Two Cells Co Ltd , 幸夫加藤 , 株式会社ツーセル
发明人： KATO YUKIO , HARA TETSUAKI , TSUJI KOICHIRO
IPC分类号： C12M1/22 , C12M1/24 , C12M3/00 , C12N5/07 , C12N5/074 , C12N5/0775 , C12N5/0789 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a culturing method for obtaining extremely more animal cells compared to a conventional method by promoting proliferation and differentiation-inducing of the cultured animal cells in the proliferation culture and differentiation-inducing culture of stem cells or the like of various kinds of animals while effectively keeping the differentiation abilities of the animal stem cells or the like under proliferation. SOLUTION: The proliferation of the cultured animal cells of various kinds of the animal cells is promoted by culturing the animal cells in the presence of an extracellular substrate derived from a mesenchymal cell, an epithelial cell, a cartilaginous precursor cell or a fibroblast. Further, the differentiation ability of the cultured animal stem cells or the like is effectively maintained under the proliferation, and the differentiation induction is promoted to enable the excellent differentiation-inducing culture of the animal stem cells to be carried out. Preferably, even serum in a low concentration can be effectively proliferated and the culture by using the human serum can be carried out by using the method of culturing the animal cells and adding various kinds of additives to a medium. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：为了提供与传统方法相比获得极其多的动物细胞的培养方法，通过促进培养的动物细胞在增殖培养物和干细胞分化诱导培养中的增殖和分化诱导像各种动物一样有效地保持动物干细胞等在增殖下的分化能力。解决方案：通过在源自间充质细胞，上皮细胞，软骨前体细胞或成纤维细胞的细胞外底物的存在下培养动物细胞来促进各种动物细胞的培养动物细胞的增殖。此外，培养的动物干细胞等的分化能力在增殖下有效地保持，促进分化诱导，使得能够进行优异的分化诱导的动物干细胞的培养。优选地，即使是低浓度的血清也可以有效地增殖，并且可以通过使用培养动物细胞并向培养基中添加各种添加剂的方法来进行使用人血清的培养。版权所有（C）2006，JPO＆NCIPI

9. 发明专利

JPS6133293A Filter 失效
标题翻译：过滤
公开(公告)号：JPS6133293A
公开(公告)日：1986-02-17
申请号：JP15263784
申请日：1984-07-23
申请人： Yukio Kato
发明人： KATO YUKIO
IPC分类号： C02F3/06
CPC分类号： Y02W10/15
摘要： PURPOSE: To prevent the deterioration in function of a filter by providing a discharge port to a bottom surface of the part close to the bottom surface and forming a filter medium of filter medium layer in the discharge side with ballast, broken stone or wood wool-like material.
CONSTITUTION: One end of the inside of vessel of a main body 1 is partitioned at right angles for the flow direction by providing a perforated plate 4 perpendicularly to provide an inflow chamber A between the perforated plate 4 and the wall surface. The part between the plate 4 and the wall surface of the other end is partitioned to form a discharge chamber C by providing perpendicularly a perforated plate 5 parallel to the perforated plate 4 and a filter medium 6 is incorporated between the perforated plates 4, 5 to form a filter medium layer B and an inflow pipe 2 communicating to a circulation pump is perpendicularly provided in the inflow chamber A and an inflow port 8 is opened in the position immersed into water. When the circulation pump is actuated, water is flowed into a water storage chamber A from the inflow port 8 of the inflow pipe 2 and passed through the filter medium layer B in the lateral direction from the water flow holes 7 of the perforated plate 4 and returned through a discharge port 3 to be circulated.
COPYRIGHT: (C)1986,JPO&Japio
摘要翻译：目的：通过在靠近底面的部分的底面设置排出口，在排出侧形成过滤介质层的过滤介质，防止过滤器功能的劣化，其中有压载物，碎石或木棉，像材料。构成：主体1的容器内部的一端通过垂直设置多孔板4而在多孔板4和壁面之间形成流入室A，从而沿流动方向成直角分隔。板4与另一端的壁面之间的部分通过垂直地提供与多孔板4平行的多孔板5而分隔成一个排放室C，并且过滤介质6被结合在多孔板4,5之间形成过滤介质层B和与循环泵连通的流入管2在流入室A中垂直设置，并且在浸入水中的位置打开流入口8。当循环泵被致动时，水从流入管2的流入口8流入储水室A，并从多孔板4的水流孔7沿横向从过滤介质层B流过，通过排放口3返回以进行循环。

10. 发明专利

JP2006204200A Agent for accelerating ossification and/or chondrification of mesenchymal stem cell and method for accelerating ossification and/or chondrification 有权
标题翻译：用于加速卵母细胞的移植和/或重建的代理和用于加速移植和/或修复的方法
公开(公告)号：JP2006204200A
公开(公告)日：2006-08-10
申请号：JP2005021336
申请日：2005-01-28
申请人： Yukio Kato , Two Cells Co Ltd , 幸夫加藤 , 株式会社ツーセル
发明人： KATO YUKIO , NISHIMURA MASAHIRO , NISHIMURA HARUKI , TSUJI KOICHIRO , SEKIYA KENSUKE
IPC分类号： A61K35/12 , A61K36/48 , A61K38/00 , A61L27/00 , A61P19/00 , A61P19/02 , A61P19/08 , A61P19/10 , A61P29/00 , C12N5/07 , C12N5/074 , C12N5/0775
摘要： PROBLEM TO BE SOLVED: To provide a method for accelerating regeneration of a bone or cartilage, and to provide a medicament for the same. SOLUTION: This medicament for accelerating ossification and/or chondrification of a mesenchymal stem cell contains lectin as an active ingredient. The medicament is preferably added to a culture medium of the mesenchymal stem cell. COPYRIGHT: (C)2006,JPO&NCIPI

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