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71. 发明专利

JP2003344396A Method of immobilizing orientation-controlled protein and method of immobilizing aligned protein utilizing the same 有权
标题翻译：定向方向控制的蛋白质的方法和使用其对齐的蛋白质的方法
公开(公告)号：JP2003344396A
公开(公告)日：2003-12-03
申请号：JP2002148950
申请日：2002-05-23
申请人： National Institute Of Advanced Industrial & Technology , 独立行政法人産業技術総合研究所
发明人： IWAKURA MASAHIRO , HIROTA KIYONORI
IPC分类号： G01N33/53 , C07K14/00 , C07K17/00 , C07K17/06 , C12N11/00 , G01N37/00
CPC分类号： C07K17/06 , C07K14/001
摘要： PROBLEM TO BE SOLVED: To provide a method of effectively producing an immobilized protein of which only carboxy terminal is immobilized.
SOLUTION: The method of producing the immobilized protein is that the protein shown in general formula (1) NH
2 -R
1 -COOH (1) is immobilized on an immobilizing carrier, and the protein having a sulfhydryl group shown in general formula (2) NH
2 -R
1 -CONH-R
2 -CO-NH-CH(CH
2 -SH)-CO-NH-R
3 -COOH (2) is produced and is absorbed and immobilized on the immobilizing carrier shown in general formula (3) NH
2 -Y (3) by an ion interaction under a neutral condition, more over a sulfhydryl group in a cysteine residue in the protein shown in the general formula (2) is cyanated by a cyanate regent, thereby converting the sulfhydryl group into cyanocysteine residue. The immobilized protein shown in general equation (4) NH
2 -R
1 -CO-NH-R
2 -CO-NH-Y (4). [wherein R
1 and R
2 are arbitrary amino acid sequence, R
3 is strongly negatively charged in the vicinity of neutral, and capable of making equipotential points of NH
2 -R
1 -CONH- R
2 -CO-NH-CH(CH
2 -SH)-CO-NH-R
3 -COOH acid, and the Y represents arbitrary immobilizing carrier].
COPYRIGHT: (C)2004,JPO
摘要翻译：待解决的问题：提供一种有效地制备仅固定羧基末端的固定化蛋白质的方法。解决方案：制备固定化蛋白质的方法是将通式（1）所示的蛋白质-NHOH（1）固定在固定载体和具有通式（2）所示的巯基的蛋白质NH 2 SBR-1 SBB-1-SBB-C-H 2 -S- 产生CO-NH-CH（CH 2 -SH）-CO-NH-R SB 3 -COOH（2），并将其吸附固定在固定载体上在通式（2）中所示的蛋白质中的半胱氨酸残基中的巯基更多的是通过式（3）通过在中性条件下的离子相互作用的NH（2S）-Y（3）通过氰酸盐摄取，从而将巯基转化为氰化半胱氨酸残基。通式（4）中所示的固定化蛋白质NH-SB 2 -R SB 1 -CO-NH-R SB 2 -CO-NH-Y（ 4）。 [其中R 1 和R SB 2是任意的氨基酸序列，R 3 SB 3在中性附近被强烈带负电荷，并能够制备 NH 2 SB-2的等位点 - SB-2 SB-2-SB-2（SBB）2 - / - -SH）-CO-NH-R SB 3 -COOH酸，Y代表任意的固定载体]。版权所有（C）2004，JPO

72. 发明专利

JP2003523777A 失效
公开(公告)号：JP2003523777A
公开(公告)日：2003-08-12
申请号：JP2000527349
申请日：1998-07-08
IPC分类号： A61M1/36 , A61L2/00 , A61M1/02 , B01D15/00 , B01D15/08 , B01J20/20 , B01J20/26 , B01J20/28 , C07D219/10 , C07D405/12 , C07D493/04 , C07F9/64 , C12N11/00 , C12N11/08 , C12N11/16

73. 发明专利

JP2003516757A 失效
公开(公告)号：JP2003516757A
公开(公告)日：2003-05-20
申请号：JP2001545566
申请日：2000-12-12
IPC分类号： A23L1/308 , A23L33/20 , A61K31/702 , A61K31/715 , A61P1/04 , A61P3/10 , A61P3/14 , A61P19/10 , A61P29/00 , A61P31/04 , A61P35/00 , A61P37/04 , C07H3/04 , C07H3/06 , C08B37/00 , C12N1/20 , C12N9/24 , C12N9/42 , C12N11/00 , C12P19/00 , C12P19/14 , C12R1/125 , A23L1/307
摘要： The invention relates to methods for the hydrolysis of galactomannan compounds and various uses of the hydrolysate.

74. 发明专利

JP3393212B2 失效
公开(公告)号：JP3393212B2
公开(公告)日：2003-04-07
申请号：JP2000136721
申请日：2000-05-10
IPC分类号： G01N33/18 , C02F1/00 , C02F3/12 , C12N1/00 , C12N1/20 , C12N11/00 , C12Q1/06

75. 发明专利

JP2003000291A Method for hydrolyzing oil and fat 有权
标题翻译：水解油和脂肪的方法
公开(公告)号：JP2003000291A
公开(公告)日：2003-01-07
申请号：JP2001190335
申请日：2001-06-22
申请人： Kao Corp , 花王株式会社
发明人： KOMATSU TOSHITERU , YAMAGUCHI HIROAKI , YAMADA NAOTO
IPC分类号： C12N9/20 , C12N11/00 , C12P7/64
CPC分类号： C12P7/6418 , C12N9/20 , C12N11/00
摘要： PROBLEM TO BE SOLVED: To provide a method for hydrolyzing oil and fat comprising reaction between an oily phase substrate and an aqueous phase substrate in a highly active immobilized enzyme-packed bed under specified conditions so as to more effectively manifest the activity of the immobilized enzyme, raise the efficiency of the hydrolytic reaction to effect higher productivity and improve the durability of the immobilized enzyme as well. SOLUTION: This method for hydrolyzing oil and fat comprises mixedly feeding the oily phase substrate and the aqueous phase substrate together into the immobilized enzyme-packed bed and making the hydrolytic reaction a reaction between the oily phase substrate and the aqueous phase substrate on the surface of the immobilized enzyme under such a fluid feed condition that the shearing stress factor (τw) represented by the formula (1): τw=(ΔP/ L)×dp×ε/(1-ε) comes to 1×10 to 1.4×10 MPa (where, ΔP is the maximum pressure loss (MPa) during the hydrolytic reaction in the packed bed; L is the thickness (m) of the packed bed; dp is the weight-based average particle size of the packed immobilized enzyme; and ε is the porosity of the packed bed).
摘要翻译：要解决的问题：提供一种水解油脂的方法，其包括在特定条件下在高活性固定化酶填充床中的油相底物和水相底物之间的反应，以更有效地显示固定化酶的活性提高水解反应的效率，从而提高生产率，提高固定化酶的耐久性。解决方案：这种水解油脂的方法包括将油相底物和水相底物混合进入固定化酶填充床中，使水解反应在油相底物和水相底物的表面上反应在由式（1）表示的剪切应力因子（τw）：τw=（ΔP/ L）×dp×ε/（1-ε）的流体供给条件下的固定化酶达到1×10 <-4 > 1.4×10 -3 MPa（其中ΔP是填充床水解反应期间的最大压力损失（MPa）; L是填充床的厚度（m）; dp是基于重量的平均值填充固定化酶的粒度;ε是填充床的孔隙率）。

76. 发明专利

JP2002520066A 失效
公开(公告)号：JP2002520066A
公开(公告)日：2002-07-09
申请号：JP2000560278
申请日：1999-06-29
IPC分类号： A23K1/16 , A23L29/00 , C12N11/00 , C12N11/08 , C12P7/18 , C12R1/01 , A23L1/03

77. 发明专利

JP2002507114A 失效
公开(公告)号：JP2002507114A
公开(公告)日：2002-03-05
申请号：JP54717098
申请日：1998-04-24
IPC分类号： A62D3/02 , A62D3/00 , B09C1/10 , C02F3/00 , C02F3/34 , C12N11/00 , C12N11/02 , C12N11/08 , C12N11/14 , C12P3/00 , C12R1/385 , C12S9/00

78. 发明专利

JP2002065245A METHOD AND APPARATUS FOR ACCELERATING REACTION OF IMMOBILIZED MICROORGANISM 失效
公开(公告)号：JP2002065245A
公开(公告)日：2002-03-05
申请号：JP2000253658
申请日：2000-08-24
申请人： FUJITEC KK
发明人： HIRAKAWA YASUTO , MIYAMURA JUICHI , MITSUHASHI OSAMU
IPC分类号： C12M1/40 , B01J19/00 , C12M1/04 , C12N1/00 , C12N11/00 , C12P1/00
摘要： PROBLEM TO BE SOLVED: To provide a method and an apparatus in which the removal efficiency of a substrate is good and the supplementation of required water and nutrient salts, etc., to an immobilized microorganism can simply and stably be carried out when the immobilized microorganism is reacted with the substrate contained in a gas. SOLUTION: This method for accelerating a reaction of the immobilized microorganism comprises filling a carrier in a reactional chamber comprising a central first chamber and a second chamber surrounding the first chamber formed so as to communicate at the bottom, bringing a part of the carrier into contact with a liquid culture medium, feeding the water or nutrients contained in the culture medium by capillarity of the carrier itself to the whole carrier, activating the immobilized microorganism, making the gas meander and pass through a layer of the carrier where the activated microorganism is immobilized, thereby bringing both into contact and maintaining and accelerating the reaction in the reaction of the microorganism immobilized in the carrier having pores with the substrate contained in the gas.

79. 发明专利

JP2002503142A 失效
公开(公告)号：JP2002503142A
公开(公告)日：2002-01-29
申请号：JP50115699
申请日：1998-06-05
IPC分类号： C12N11/00 , C02F1/68 , C02F3/34 , C11D1/14 , C11D3/386 , C11D7/42 , C11D11/00 , C11D17/00 , C12N11/02

80. 发明专利

JP2002028684A FILTER BED MATERIAL FOR EPIPHYTICALLY ADSORBING ORGANISM 失效
公开(公告)号：JP2002028684A
公开(公告)日：2002-01-29
申请号：JP2000212682
申请日：2000-07-13
申请人： KYODO KUMIAI SUTADEION
发明人： KASHIRO SHIZUO , KASEI SHIGERU
IPC分类号： C12N11/00 , C02F3/10
摘要： PROBLEM TO BE SOLVED: To provide an inexpensive organism epiphytically adsorbing filter bed material suitable for epiphytically adsorbing microbes and remarkably contributable to cleaning of wastewater or dirty water. SOLUTION: This organism epiphytically adsorbing filter bed material is constituted of a molding which has large porosity and is obtained by scorching a thermosetting synthetic resin 1b-stuck glass chop to obtain a reinforced glass chop 1, adding chaff 2 and another thermosetting synthetic resin 3 to the chop 1 to mix them, scorching the mixture and molding it so that the carbonized chaff 2 is integrally bonded to the chop 1 while the resin 3 functions as a binder.

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