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    • 67. 发明专利
    • Method for identifying homogeneity of mesenchymal stem cells, mesenchymal stem cells obtained by the method
    • 用于鉴定细胞干细胞的均质的方法,由该方法获得的中间干细胞
    • JP2007082484A
    • 2007-04-05
    • JP2005276565
    • 2005-09-22
    • Hiroshima UnivTwo Cells Co Ltd国立大学法人広島大学株式会社ツーセル
    • KATO YUKIOKANEWA MASAMIIGARASHI AKIRAHARA MAIKOTSUJI KOICHIRO
    • C12Q1/02C12N5/074C12N5/0775C12N15/09C12Q1/68
    • PROBLEM TO BE SOLVED: To provide a method for identifying the homogeneity of mesenchymal stem cells, by which whether subcultured mesenchymal stem cells are multifunctional mesenchymal stem cells or not can be identified; and to provide mesenchymal stem cells obtained by the method. SOLUTION: The method for identifying the homogeneity of the mesenchymal stem cells comprises: a step for producing two or more clones from the cell population of subcultured mesenchymal stem cells; a step for obtaining mRNA relative intensities related to a prescribed gene group with respect to the clones and their populations; a step for determining gene expression patterns obtained by schematizing the mRNA relative intensity groups of the obtained clones and their populations with respect to the gene groups; and a step for judging the homogeneity of the population from the obtained gene expression patterns. COPYRIGHT: (C)2007,JPO&INPIT
    • 待解决的问题:提供用于鉴定间充质干细胞的同质性的方法,其中是否传代培养的间充质干细胞是否是多功能间充质干细胞; 并提供通过该方法获得的间充质干细胞。 解决方案:用于鉴定间充质干细胞的均匀性的方法包括:从传代培养的间充质干细胞的细胞群中产生两个或多个克隆的步骤; 相对于克隆及其种群获得与规定基因组相关的mRNA相对强度的步骤; 通过对获得的克隆的mRNA相对强度组及其群体相对于基因组进行示意图来确定获得的基因表达模式的步骤; 以及从获得的基因表达模式中判断群体的均一性的步骤。 版权所有(C)2007,JPO&INPIT