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    • 60. 发明专利
    • Method for producing cultured cell for therapy
    • 用于生产用于治疗的培养细胞的方法
    • JP2010046058A
    • 2010-03-04
    • JP2009172191
    • 2009-07-23
    • Two Cells Co Ltd株式会社ツーセル
    • NISHINA HIROMICHIOKADA HIRONORIKUMAGAI NORIOYANAGA HIROKO
    • C12N5/07A61K35/28A61K35/36A61K35/44A61L27/00A61P1/16A61P3/10A61P9/10A61P13/12A61P17/02A61P25/00A61P25/16A61P25/28
    • PROBLEM TO BE SOLVED: To provide a method for producing a highly safe cultured cell for therapy by which the culture can be carried out without using various kinds of risk factors and nutrition-supporting cells.
      SOLUTION: The method for producing the cultured cell for the therapy includes collecting a tissue from a mammal, treating the tissue with a proteolytic enzyme, treating the resultant product with an inhibitor of the proteolytic enzyme wherein the treatment is carried out with the inhibitor of the proteolytic enzyme in an amount excessive to the theoretical amount required for deactivating the activity of the used proteolytic enzyme, by using a medium having low calcium ion concentration, and carrying out acclimatizing culture carried out by adding a new medium and thereafter removing a part thereof when replacing the medium.
      COPYRIGHT: (C)2010,JPO&INPIT
    • 待解决的问题:提供一种用于生产高度安全的用于治疗的培养细胞的方法,其可以在不使用各种危险因素和营养支持细胞的情况下进行培养。 解决方案:用于制备用于治疗的培养细胞的方法包括从哺乳动物收集组织,用蛋白水解酶处理组织,用蛋白水解酶的抑制剂处理所得产物,其中用 通过使用具有低钙离子浓度的培养基,使蛋白水解酶的抑制剂的量超过使所用蛋白水解酶的活性失活所需的理论量,并且通过加入新培养基进行驯化培养,然后除去 更换介质时的一部分。 版权所有(C)2010,JPO&INPIT