专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

41. 发明专利

JPH06339397A METHOD FOR REMOVING REDUCTIVE SUBSTANCE IN BIOLOGICAL SPECIMEN 失效
公开(公告)号：JPH06339397A
公开(公告)日：1994-12-13
申请号：JP21421893
申请日：1993-08-30
申请人： TOYO BOSEKI
发明人： WATANABE HARUO , HAYASHI YUZO , ANDO MINORU
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/34
摘要： PURPOSE:To conduct a determination in high accuracy by eliminating the influence of reductive substances in a biological specimen. CONSTITUTION:In such a system as to optically determine the components in a biological specimen through the oxidative color reaction of peroxidase, the objective method is characterized by eliminating reductive substances hampering the reaction using an electron acceptor selected from the group consisting of benzoic acid, p-hydroxybenzoic acid, p-aminobenzoic acid and dibromophenol.

42. 发明专利

JPH06315400A REAGENT FOR MEASURING ACTIVITY OF ESTER BOND HYDROLYASE 失效
公开(公告)号：JPH06315400A
公开(公告)日：1994-11-15
申请号：JP33193193
申请日：1993-12-27
申请人： TOYO BOSEKI
发明人： TEJIMA SHINICHI , HAYASHI YUZO , ANDO MINORU
IPC分类号： C12Q1/44
摘要： PURPOSE:To obtain the reagent for measuring activity of ester bond hydrolyase capable of accurately and simply measuring the activity of ester bond hydrolyase in a high measuring sensitivity and in a short time, containing a specific nitrophenol derivative as a substrate, capable of carrying out rate assay of an ester bond hydrolase. CONSTITUTION:A compound of the formula [X is halogen; R is SO3H; (1) is 1-4 integer; (m) is 1-4 integer; (n) is 0-3 integer; l+m+n (R is 1-20C monofunctional aliphatic group)] (e.g. 2-sulfo-4-nitro-6- chloro-phenyl palmitate) is dissolved in 0.1M phosphoric acid buffer solution (pH 7.0) into 2mM concentration to give an ester bond hydrolase activity reagent. This reagent is added to a specimen containing the ester bond hydrolyase (e.g. lipase), reacted at 37 deg.C, the amount of a formed detectable substance is measured by measuring the absorbance of the substance by a spectrophotometer to determine the activity of ester bond hydrolyase in a short time, accurately and in a high sensitivity.

43. 发明专利

JPH03995B2 失效
公开(公告)号：JPH03995B2
公开(公告)日：1991-01-09
申请号：JP17560684
申请日：1984-08-22
申请人： TOYO BOSEKI
发明人： KIKUCHI TOSHIRO , AISUI SHIGENORI , ANDO MINORU
IPC分类号： C12N9/04 , C12R1/225

44. 发明专利

JPH0236237B2 失效
公开(公告)号：JPH0236237B2
公开(公告)日：1990-08-16
申请号：JP1131782
申请日：1982-01-26
申请人： TOYO BOSEKI
发明人： KIKUCHI TOSHIRO , KAMISAKA MASAYO , ANDO MINORU , NAKAGIRI YOSHITAKA
IPC分类号： C12Q1/46 , C12Q1/26

45. 发明专利

JPS6368078A NOVEL LUMINOUS BACTERIUM LUCIFERASE AND PRODUCTION THEREOF 失效
公开(公告)号：JPS6368078A
公开(公告)日：1988-03-26
申请号：JP21366786
申请日：1986-09-10
申请人： TOYO BOSEKI
发明人： WATANABE HARUO , ANDO MINORU , KASAI YOSHIO , MATSUI KUNIO
IPC分类号： C12N9/02 , C12R1/01
摘要： PURPOSE:To obtain luciferase emitting light with an oxidizing agent without adding a luminous substrate, by collecting bacterium luciferase from a cultivated cell of a specific luminous bacterium by purification means including hydrophobic chromatography. CONSTITUTION:A luminous bacterium producing bacterium luciferase is cultivated and the aimed bacterium luciferase is collected from the cultivated cell by purification means including hydrophobic chromatography. Consequently, the bacterium luciferase having the following physical and chemical properties (i) and (ii) is obtained. (i) The bacterium luciferase emits light by adding of an oxidizing agent in a state of being oxidized with a reducing agent or of being optically reduced in the presence of an optically reducing catalyst without providing a substrate. (ii). The bacterium luciferase develops ability of being optically reduced by light irradiation and the bacterium luciferase emits light by addition of oxidizing agent, is oxidized, optically reduced again and emits light. Luminous bacteria such as Vibrio fischeri, Photobacterium phosphoreum, etc., may be cited as the bacterium producing the bacterium luciferase.

46. 发明专利

JPS633594B2 失效
公开(公告)号：JPS633594B2
公开(公告)日：1988-01-25
申请号：JP6262883
申请日：1983-04-08
申请人： TOYO BOSEKI
发明人： IWAKI YASUO , AISUI SHIGENORI , ANDO MINORU
IPC分类号： C12N9/20 , C12R1/38

47. 发明专利

JPS6155946B2 失效
公开(公告)号：JPS6155946B2
公开(公告)日：1986-11-29
申请号：JP12656878
申请日：1978-10-13
申请人： TOYO BOSEKI
发明人： ANDO MINORU , IWAKI YASUO , SHIBATA HIDEJI , KIKUCHI TOSHIRO
IPC分类号： C12N9/02 , C12R1/01 , C12R1/025 , C12R1/06 , C12R1/185 , C12R1/265 , C12R1/37 , C12R1/38 , C12R1/425

48. 发明专利

JPS61237060A ASSAY FOR COMPONENTS OF LIVING ORGANISM BY CHEMICAL LUMINESCENCE METHOD 失效
公开(公告)号：JPS61237060A
公开(公告)日：1986-10-22
申请号：JP7906785
申请日：1985-04-12
申请人： TOYO BOSEKI
发明人： WATANABE HARUO , MITSUHIDA NOBORU , ANDO MINORU
IPC分类号： G01N33/52 , C12Q1/00 , C12Q1/26 , C12Q1/62 , G01N33/76
摘要： PURPOSE:To assay the activity of a substrate or an enzyme, by converting hydrogen peroxide generated from an enzyme reaction product due to the substrate or the enzyme in an organism sample into fluorescent substance to which diester oxalate compounds and hydrogen peroxide are added. CONSTITUTION:An enzyme is made to act upon a substrate as component of a living organism or a substrate is made to act upon an enzyme and finally an oxidation enzyme is applied via a single or multiple enzyme reaction processes to form hydrogen peroxide. Then, an non-fluorescent substance and an oxidation catalysis are made to act upon hydrogen peroxide to form a fluorescent substance. Then, diester oxalate and hydrogen peroxide are made to act upon the fluorescent substance to generate light and the intensity of light is measured.

49. 发明专利

JPS61198062A ENZYME IMMUNOASSAY FOR ERYTHROPOIETIN 失效
公开(公告)号：JPS61198062A
公开(公告)日：1986-09-02
申请号：JP3968785
申请日：1985-02-28
申请人： TOYO BOSEKI
发明人： MATSUMOTO HIROJI , TAMABUCHI KEIKO , ANDO MINORU
IPC分类号： G01N33/53 , G01N33/543 , G01N33/74
摘要： PURPOSE:To measure the enzyme immunity of erythropoietin by forming an antibody-erythropoietin-labeling material composite on an antibody-conjugated insoluble base then measuring the quantity of the labeling material conjugated onto the base. CONSTITUTION:The antibody-conjugated insoluble base formed by conjugating the antibody reacting specifically with erythropoietin (EPO) on the insoluble base and a specimen liquid contg. the EPO are brought into reaction to conjugate the EPO onto the antibody-conjugated insoluble base. The same is then brought into reaction with the labeling material labeling the antibody reacting specifically with the EPO with enzyme by which the antibody-EPO-labeling material composite is formed on the antibody-conjugated insoluble base. The quantity of the labeling material conjugated on the base is then measured or the quantity of the liberated labeling material which is not conjugated is measured. The quick enzyme immunomeasurement of the EPO in an ordinary inspection room at a low cost with high sensitivity is thus made possible.

50. 发明专利

JPS61173799A METHOD OF DETERMINING ACTIVITY OF SUBSTRATE OR ENZYME 失效
公开(公告)号：JPS61173799A
公开(公告)日：1986-08-05
申请号：JP1623785
申请日：1985-01-29
申请人： TOYO BOSEKI
发明人： TAMAI KIYOMI , HAYASHI YUZO , ANDO MINORU
IPC分类号： C12Q1/28 , C12Q1/26 , C12Q1/30
摘要： PURPOSE:In determining activity of substrate or enzyme in a test specimen by measuring formed hydrogen peroxide with peroxidase, to improve measuring accuracy and to make automatic analysis applicable, by using catalase having

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式