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41. 发明专利

JP2009112315A Method for isolating polyhydroxyalkanoate from plant 审中-公开
标题翻译：从植物中分离聚羟基烷基酯的方法
公开(公告)号：JP2009112315A
公开(公告)日：2009-05-28
申请号：JP2009045960
申请日：2009-02-27
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： MARTIN DAVID P , PEOPLES OLIVER P , WILLIAMS SIMON F
IPC分类号： A01H1/00 , C12P7/62 , C08G63/89 , C08H99/00 , C12N5/10 , C12N15/09
CPC分类号： C08G63/89 , C12P7/625
摘要： PROBLEM TO BE SOLVED: To provide methods for separating polyhydroxyalkanoates ("PHA") from plants, such as transgenic oil crop plants.
SOLUTION: To isolate the PHA, in one embodiment, a biomass derived from an oil crop plant is pre-processed, for example by grinding, crushing or rolling. Then the oil is extracted from the biomass with a first solvent (in which solvent the oil is soluble and the PHA are not highly soluble) to remove the oil. Then the biomass can be extracted with a second solvent (in which solvent the PHA is soluble) to separate the PHA from the biomass. Alternatively, the PHA-containing biomass is treated with a chemical or biochemical agent to chemically transform the PHA into a PHA derivative. Then the PHA derivative is separated from the mixture by using, for example, a physical separation process.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供从植物如转基因油料作物植物中分离聚羟基链烷酸酯（“PHA”）的方法。解决方案：为了分离PHA，在一个实施方案中，来自油料作物植物的生物质被预处理，例如通过研磨，破碎或轧制。然后用第一溶剂（其中油溶于溶剂，PHA不高度溶解）从生物质中提取油，以除去油。然后生物质可以用第二溶剂（其中PHA溶于其中的溶剂）提取，以将PHA与生物质分离。或者，用化学或生化试剂处理含PHA的生物质，以将PHA化学转化为PHA衍生物。然后通过使用例如物理分离方法将PHA衍生物与混合物分离。版权所有（C）2009，JPO＆INPIT

42. 发明专利

JP2009082147A Biological system for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxy acid 审中-公开
标题翻译：含有4-羟基酸的聚羟基烷酸酯聚合物的生物体系
公开(公告)号：JP2009082147A
公开(公告)日：2009-04-23
申请号：JP2008328226
申请日：2008-12-24
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： HUISMAN GJALT W , SKRALY FRANK A , MARTIN DAVID P , PEOPLES OLIVER P
IPC分类号： C12N1/21 , C12N15/09 , C08G63/06 , C12N9/02 , C12N9/10 , C12N15/53 , C12N15/54 , C12N15/82 , C12P7/52 , C12P7/62 , C12R1/19
CPC分类号： C12N9/0008 , C08G63/06 , C12N9/1029 , C12N9/1096 , C12N15/8243 , C12P7/625
摘要： PROBLEM TO BE SOLVED: To provide the technology and process for stably making a transgenic organism synthesizing PHA including 4-hydroxy-butyric acid as only one component or as co-monomer by using the recombination process in which another gene is introduced into the transgenic PHP producer, in order to create a new strain synthesizing the substitute PHA monomer (for example, 4-HB). SOLUTION: A recombinant host that stably incorporates the gene encoding different kinds of enzyme selected from polyhydroxyalkanoate synthase and 4HB-CoA transferase into the genome. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供用于稳定制备转基因生物的技术和方法，其通过使用引入另一种基因的重组方法将包含4-羟基 - 丁酸的PHA作为仅一种成分或作为共聚单体合成转基因PHP生产者，以产生合成替代PHA单体（例如4-HB）的新菌株。解决方案：将编码不同种类的选自聚羟基链烷酸合酶和4HB-CoA转移酶的基因的基因稳定并入基因组的重组宿主。版权所有（C）2009，JPO＆INPIT

43. 发明专利

JP2008248258A Method for isolating polyhydroxyalkanoate from plant 有权
标题翻译：从植物中分离聚羟基烷基酯的方法
公开(公告)号：JP2008248258A
公开(公告)日：2008-10-16
申请号：JP2008180815
申请日：2008-07-10
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： MARTIN DAVID P , PEOPLES OLIVER P , WILLIAMS SIMON F
IPC分类号： A01H1/00 , C08G63/89 , C08H99/00 , C12N5/10 , C12N15/09 , C12P7/62
CPC分类号： C08G63/89 , C12P7/625
摘要： PROBLEM TO BE SOLVED: To provide a method for separating polyhydroxyalkanoates ("PHAs") from plants such as a transgenic oil crop plant.
SOLUTION: In an embodiment of the method, to isolate the PHAs, a biomass derived from an oil crop plant is preprocessed by, for example, grinding, crushing or rolling. Oil is then extracted from the biomass by using a first solvent (in which the oil is soluble and the PHAs are not highly soluble) to remove the oil. Then, the biomass can be extracted by using a second solvent (in which the PHAs are soluble) to separate the PHAs from the biomass. Alternatively, the PHA-containing biomass is treated with a chemical or biochemical agent to chemically transform the PHA into a PHA derivative. The PHA derivative is then separated from the mixture by using a physical separation process.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供从植物如转基因油料作物植物中分离聚羟基链烷酸酯（“PHAs”）的方法。解决方案：在该方法的一个实施方案中，为了分离PHAs，通过例如研磨，破碎或轧制来预处理来自油料作物的生物质。然后通过使用第一溶剂（其中油可溶而PHAs不是高度可溶的））从生物质中提取油以除去油。然后，可以通过使用第二溶剂（其中可溶于PHAs）来提取生物质，以将PHAs与生物质分离。或者，用化学或生化试剂处理含PHA的生物质，以将PHA化学转化为PHA衍生物。然后通过使用物理分离方法将PHA衍生物与混合物分离。版权所有（C）2009，JPO＆INPIT

44. 发明专利

JP2008532553A Chemically inducible expression of the biosynthetic pathway 审中-公开
公开(公告)号：JP2008532553A
公开(公告)日：2008-08-21
申请号：JP2008502042
申请日：2006-03-16
申请人：メタボリックス, インコーポレイテッド
发明人：ローラリンクールツ， , クリスティディー．スネル， , オリバーピー．ピープルズ，
IPC分类号： C12N15/09 , C12N5/10 , C12P7/62
CPC分类号： C12N15/8247 , C12N15/8237 , C12N15/8243
摘要：多酵素生合成経路において酵素をコード化する同義遺伝子の導入方法および構築物が提供される。１つの実施形態において、これらの構築物は、各々が誘導プロモーターの制御下にあり、かつ各々がポリアデニル化シグナルを有する、２またはそれ以上の酵素コード化遺伝子を含有する。これらの構築物は、形質転換植物を生産するために用いられる。これらの植物において、酵素の発現は、化学的誘導剤が適用された時に増加され、導入遺伝子によってコード化された一連の酵素の生合成産物が生成される。１または複数の導入遺伝子から発現されたアクチベータ分子もしくは複合体によって活性化される１またはそれ以上のプロモーターの制御下にある２またはそれ以上の酵素コード化遺伝子を含有する構築物が用いられてもよい。導入遺伝子はこれら自体が、１またはそれ以上の誘導プロモーターの制御下にあり、化学物質の外部からの適用後にスイッチオンされる。

45. 发明专利

JP4121541B2 Production of polyhydroxyalkanoates of medium chain length of the fatty acid biosynthesis pathway 有权
公开(公告)号：JP4121541B2
公开(公告)日：2008-07-23
申请号：JP2007153418
申请日：2007-06-08
申请人：メタボリックス,インコーポレイテッド
发明人：ピー．ピープルスオリバー , ディー．スネルクリスティ , アクインステファニー
IPC分类号： A01H5/00 , C12Q1/44 , A01H1/00 , C12N1/21 , C12N9/00 , C12N9/10 , C12N9/16 , C12N15/09 , C12N15/54 , C12N15/55 , C12N15/82 , C12P7/42 , C12Q1/02 , C12Q1/25 , C12Q1/48
CPC分类号： C12N9/93 , C12N9/00 , C12N9/10 , C12N9/1029 , C12N9/16 , C12N15/8243
摘要： Methods for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, have been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase with substrate specificity for medium chain length 3-hydroxy fatty acids, and a medium chain length PHA synthase, has been developed. Acyl CoA synthetase activity can be supplied either by the endogenous acyl CoA synthetase of the host organism, when sufficiently expressed, or the host organism's activity can be supplemented by the expression of a recombinant acyl CoA synthetase gene. New strategies are described for plant based PHA production in the chloroplasts, cytosol, and peroxisomes of biomass crops as well as the plastids, cytosol, and peroxisomes of oil seed crops.

47. 发明专利

JP3373841B2 Polyhydroxyalkanoate molding composition 失效
公开(公告)号：JP3373841B2
公开(公告)日：2003-02-04
申请号：JP2000504195
申请日：1998-07-22
申请人：メタボリックス,インコーポレイテッド
发明人：アナエゴジー， , ダニエルホロウィッツ， , エドワードミュラー，
IPC分类号： C12P7/62 , C04B35/622 , C08J5/00 , C08K3/00 , C08K3/22 , C08K3/24 , C08L67/04 , C08L101/00 , C08L101/16 , C12R1/40
CPC分类号： C04B35/62655 , B22F1/0074 , B22F3/1021 , B22F3/20 , B22F3/22 , B22F3/225 , C04B35/111 , C04B35/4682 , C04B35/632 , C04B35/63408 , C04B35/6342 , C04B35/63432 , C04B35/6346 , C04B35/63464 , C04B41/009 , C04B41/5116 , C04B41/88 , C04B2235/604 , C08K3/01 , C08K3/22 , C08K3/24 , C08L67/04 , C08L101/00 , C08L2666/02 , C08L67/02 , C04B33/00 , C04B41/4539
摘要： Molding compositions including polyhydroxyalkanoates are provided. The use of polyhydroxyalkanoates as a binder in molding compositions provides improved binder removal in the finished molded product, and offers a wide range of physical properties suitable for use in a variety of processing conditions. The composition preferably includes a powdered material, such as a metal powder, ceramic powder, or blend, admixed with a polyhydroxyalkanoate binder. The compositions are useful in powder processing techniques, such as injection molding, slip casting, tape casting, or extrusion.

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