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11. 发明专利

JPS58152480A PREPARATION OF ALDEHYDE DEHYDROGENASE BOUND TO MEMBRANE 失效
公开(公告)号：JPS58152480A
公开(公告)日：1983-09-10
申请号：JP3518582
申请日：1982-03-08
申请人： NAKANO SUTEN KK
发明人： TAYAMA KENJI , FUKAYA MASAHIRO , FUJIYAMA SEIICHI , MASAI HIROSHI
IPC分类号： C12N9/04
摘要： PURPOSE:To prepare an aldehyde dehydrogenase bound to a membrane in high yield, by inoculating a strain belonging to acetic acid bacteria into a nutrient culture medium containing ethanol, and cultivating the strain. CONSTITUTION:A strain, e.g. Acetobacter MK-17 strain (FERM-P No.5747), belonging to acetic acid bacteria is inoculated into an ordinary culture medium for the acetic acid bacteria containing a carbon source, e.g. glucose, a nitrogen source, e.g. peptone, a salt, e.g. K, Ca or Mg, and a minor element, e.g. vitamin or amino acid, and cultivated therein. In a period before the completion of the logarithmic growth phase of the acetic acid bacteria, 0.1-4V/V% ethanol is added to the culture medium, and aerobically cultivated at 2.0-7.0pH and 25- 38 deg.C for 20-480hr. The resultant microbial cell is then collected and suspended in a buffer solution, destroyed and centrifuged at a low speed to remove the settling undestroyed microbial cell. The resultant supernatant liquid is then centrifuged at a high speed. A surfactant is then added to the precipitated membranous fraction to solubilize the enzyme, which is then purified by the chromatography to give the aimed aldehyde dehydrogenase bound to the membrane.

12. 发明专利

JP2571119B2 失效
公开(公告)号：JP2571119B2
公开(公告)日：1997-01-16
申请号：JP3377589
申请日：1989-02-15
申请人： NAKANO SUTEN KK
发明人： FUKAYA MASAHIRO , TAYAMA KENJI , TAMAOKI TOSHIMI , TAGAMI HARUKO , OKUMURA HAJIME , KAWAMURA KICHA
IPC分类号： C12N1/21 , C12N9/02 , C12N9/04 , C12N15/09 , C12N15/53 , C12P7/54 , C12R1/02

13. 发明专利

JP2564147B2 失效
公开(公告)号：JP2564147B2
公开(公告)日：1996-12-18
申请号：JP25388887
申请日：1987-10-09
申请人： NAKANO SUTEN KK
发明人： TODA KYOSHI , FUKAYA MASAHIRO , OKUMURA HAJIME
IPC分类号： C12J1/04

14. 发明专利

JPH0625744B2 失效
公开(公告)号：JPH0625744B2
公开(公告)日：1994-04-06
申请号：JP25385087
申请日：1987-10-09
申请人： NAKANO SUTEN KK
发明人： NANBA AKIRA , NAGAI SHIRO , FUKAYA MASAHIRO , OKUMURA HAJIME
IPC分类号： G01N27/327 , G01N27/30

15. 发明专利

JPS60188068A TRANSFORMATION OF ACETOBACTER USING DRUG-RESISTANT GENE 失效
公开(公告)号：JPS60188068A
公开(公告)日：1985-09-25
申请号：JP4298084
申请日：1984-03-08
申请人： NAKANO SUTEN KK
发明人： FUKAYA MASAHIRO , OKUMURA HAJIME , MASAI HIROSHI
IPC分类号： C12N15/09 , C12N15/74 , C12R1/02
摘要： PURPOSE:To carry out the transformation of acetobacter using a vector obtained by linking an acetobacter plasmid with a gene selected from a chloramphenicol- resistant gene and a kanamycin-resistant gene originated from the drug-resistant plasmid R-6 of Escherichia coli, based on the founding that the above genes can be manifested in the cell of acetobacter. CONSTITUTION:The plasmid pTA5001(A) separated from Acetobacter aceti or the vector plasmid pTA5001(B) is prepared, is subjected to the incision with a proper restriction enzyme using the plasmid pACYC177 (having kanamycin- and amplicillin-resistant gene) and pACYC184 (having chloramphenicol- or kanamycin-resistant gene) and to the recombination using a T4DNA ligase. A vector for acetobacter, containing chloramphenicol- or kanamycin-resistant gene and copyable in the cell of acetobacter can be prepared by this process. The transformation is carried out by using Acetobacter aceti 10-80SI (AceSS, Eth , Pro , Str ) as a receptor cell.

16. 发明专利

JPS6031469B2 失效
公开(公告)号：JPS6031469B2
公开(公告)日：1985-07-22
申请号：JP15421778
申请日：1978-12-15
申请人： NAKANO SUTEN KK
发明人： FUJAMA SEIICHI , FUKAYA MASAHIRO , HATA MORIMASA , MASAI HIROSHI , YAMADA KOKI
IPC分类号： C12J1/04

17. 发明专利

JPS58152479A PREPARATION OF ALCOHOLIC DEHYDROGENASE BOUND TO MEMBRANE 失效
公开(公告)号：JPS58152479A
公开(公告)日：1983-09-10
申请号：JP3518482
申请日：1982-03-08
申请人： NAKANO SUTEN KK
发明人： FUJIYAMA SEIICHI , TAYAMA KENJI , FUKAYA MASAHIRO , MASAI HIROSHI
IPC分类号： C12N9/04
摘要： PURPOSE:To prepare an alcoholic dehydrogenase bound to a membrane, by ionculating a strain belonging to acetic acid bacteria into a nutrient culture medium containing ethanol, and cultivating the acetic acid bacteria. CONSTITUTION:A strain, e.g. Acetobacter MK-06 (FERM-P No.6173), belonging to acetic acid bacteria, and capable of producing an alcoholic dehydrogenase bound to a membrane is inoculated into an ordinary culture medium for the acetic acid bacteria containing a carbon source, e.g. glucose, a nitrogen source, e.g. peptone, a salt, e.g. K, Ca or Mg, and a minor element, e.g. vitamin or amino acid, and cultivated therein. In a period before the completion of the logarithmic growth phase of the acetic acid bacteria, 0.1-4V/V% ethanol is added to the culture medium and aerobically cultivated at 2.0-7.0pH and 25- 38 deg.C for 20-480hr. The microbial cell is then collected, suspended in a buffer solution, destroyed and centrifuged at a low speed to remove the undestroyed microbial cell. The resultant supernatant liquid is then centrifuged at a high speed, and a surfactant is added to the centrifuged membranous fraction to solubilize the enzyme, which is then purified by the chromatography to give the aimed alcoholic dehydrogenase bound to the membrane.

18. 发明专利

JPS57144221A ANTITUMOR SUBSTANCE 失效
公开(公告)号：JPS57144221A
公开(公告)日：1982-09-06
申请号：JP2940481
申请日：1981-03-03
申请人： NAKANO SUTEN KK
发明人： FUJIYAMA SEIICHI , FUKAYA MASAHIRO , HATA MORIMASA , MIZUKAMI HIROYUKI , YAMADA KOUKI
IPC分类号： A61K35/74 , A61P35/00
摘要： PURPOSE:An antitumor substance that contains the cell wall fraction of cell bodies of a strain in Gluconobacter as an active ingredient. CONSTITUTION:A strain of a bacterium in Gluconobacter such as Gluconobacter oxydans IFO-3287 is aerobically cultured at 20-45 deg.C and its cell bodies are collected by centrifugation. These cell bodies are washed with a physiological salt solution to remove components in the culture medium and products formed outside cells to collects its cell bodies, which are crushed by means of, e.g., Dyno-Mill KDL. Then, the crushed product is subjected to low-speed contrifugation to remove uncrushed cell bodies as a precipitate and the upper layer solution is subjected to high-speed contrifugation. After the insoluble substance is separated, it is dispersed in distilled water, placed in a dialysis tube to effect dialysis in 20-50 times volume of distilled water one overnight. Then, the inner dialyzate is freeze dried into a powder to give the cell wall fraction of cell bodies. The objective antitumor agent is prepared by using the product as an active ingredient. Its does to, e.g., a mouse is intraperitoneally 1-200mg/kg and hypodemically 0.5-100mg/kg.

19. 发明专利

JPS57144220A ANTITUMOR SUBSTANCE 失效
公开(公告)号：JPS57144220A
公开(公告)日：1982-09-06
申请号：JP2940381
申请日：1981-03-03
申请人： NAKANO SUTEN KK
发明人： FUJIYAMA SEIICHI , FUKAYA MASAHIRO , HATA MORIMASA , MIZUKAMI HIROYUKI , YAMADA KOUKI
IPC分类号： A61K35/74
摘要： PURPOSE:An antitumor substance that contains cell bodies of a strain in Gluconobacter as an active ingredients. CONSTITUTION:A strain of a bacterium in Gluconobacter such as Gluconobacter oxydans IFO-3287 is aerobically cultured at 20-45 deg.C and its cells are collected by centrifugation. Then, these cells are washed with a physoilogical salt solution to remove components in the culture medium and products formed outside its cells, thus collecting cell bodies. These cells are not pathogenic, relatively stable to heat and insoluble in organic solvents, thus they are treated with heat or a chemical such an formalin to give an antitumor substance. Its dose to, e.g., a mouse is intraperitoneally 1-200mg/kg and hypodermically 0.5-100mg/kg.

20. 发明专利

JPS5581582A METHOD OF MAKING VINEGAR FROM GRAIN 失效
公开(公告)号：JPS5581582A
公开(公告)日：1980-06-19
申请号：JP15367178
申请日：1978-12-14
申请人： NAKANO SUTEN KK
发明人： FUJIYAMA SEIICHI , FUKAYA MASAHIRO , HATA MORIMASA , MASAI HIROSHI , YAMADA KOUKI
IPC分类号： C12J1/04
摘要： PURPOSE:A culture mixture resulting from culturing a bacterium producing glutamic acid in a medium of saccharified liquor of grain is added to the preparation of grain for acetic fermentation or the fermentation mixture, thus producing vinegar of high quality and good taste. CONSTITUTION:In the production of vinegar from grain, a culture mixture resulting from inoculating and culturing a glutamic acid-producing bacteria in a liquid medium containing saccharified liquor from grain, nitrogen sources, when necessary, inorganic salts and trace ingredients or the liquor obtained by removing the bacterium cells from the culture mixture is added to the preparation of grain provided by a usual method or the fermentation mixture during acetic fermentation and the acetic fermentation is effected.

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