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    • 112. 发明专利
    • Reagent for measuring blood cell
    • 用于测量血液细胞的试剂
    • JPS6179163A
    • 1986-04-22
    • JP20127384
    • 1984-09-26
    • Toa Medical Electronics Co Ltd
    • SAKATA TAKASHITAKAMI TADAHIRO
    • G01N33/49G01N15/14G01N21/78G01N33/48G01N33/50G01N33/80
    • G01N33/80
    • PURPOSE:To make possible an exact measurement by kinds by constituting the reagent of a spheroidizing agent consisting of a cationic or a nonionic surface active agent expressed by the specific chemical formula, fixing agent and basic fluorescent dye. CONSTITUTION:The reagent is constituted with the cationic surface active agent expressed by formula I or the nonionic surface active agent expressed by formula II as the spheroidizing agent for spheroidizing red blood cells, the prescribed quantity of glutaraldehyde as the fixing agent for maintaining the state of the spherodized blood cells, the basic fluorescent dye for dyeing the blood cells, alkali metallic salt for making isotonic the entire part of the soln. and sodium hydroxide as a buffer agent. When such reagent is mixed with anti-clotting whole blood sample, the red blood cells and reticulocytes are efficiently spheroidized and dyed. The discrimination of the scattered light intensity of the red blood cell groups and platelet groups is thus made easy and the exact measurement of the cell groups of the whole blood by kinds is made possible.
    • 目的:通过构成由特定化学式表示的阳离子或非离子表面活性剂构成的球化剂的试剂,固色剂和碱性荧光染料,可以精确地进行各种测定。 构成:试剂由式I表示的阳离子表面活性剂或由式II表示的非离子表面活性剂构成,用作球状红细胞的球化剂,规定量的戊二醛作为维持状态的固定剂 球状血细胞,用于染色血细胞的碱性荧光染料,碱金属盐,用于使溶胶的整个部分等渗。 和氢氧化钠作为缓冲剂。 当这种试剂与抗凝血全血样品混合时,红细胞和网织红细胞被有效地球化和染色。 因此,能够容易地区分红细胞群和血小板组的散射光强度,能够精确地测定全血细胞群的种类。