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1. 发明专利

JP2014061489A Decontamination method of petroleum contaminated soil 有权
标题翻译：石油污染土壤的去除方法
公开(公告)号：JP2014061489A
公开(公告)日：2014-04-10
申请号：JP2012208717
申请日：2012-09-21
申请人： Ritsumeikan , 学校法人立命館 , Daiwa House Industry Co Ltd , 大和ハウス工業株式会社
发明人： KUBO MIKI , FUKUHARA YUKI , YOSHIDA KAZUO , KAWAGOE HIROKI , KAWAME YUSUKE , KUBOTA KENZO
IPC分类号： B09C1/10 , C12N1/00 , C12N1/20
摘要： PROBLEM TO BE SOLVED: To provide a decontamination method of a petroleum contaminated soil having high efficiency for decontamination of petroleum contamination, and to provide a composition for decontaminating the petroleum contaminated soil having high efficiency for decontamination of petroleum contamination.SOLUTION: The decontamination method of a petroleum contaminated soil includes a step of adjusting a content of all carbon in the petroleum contaminated soil (excluding a carbon content of petroleum) to 10,000 to 60,000 mg/kg, and C/N ratio in the petroleum contaminated soil to 8 to 16 by adding an organic material containing petroleum-degrading bacteria to the petroleum contaminated soil. A composition for decontaminating the petroleum contaminated soil contains petroleum-degrading bacteria and has a content of all carbon of 150,000 mg/kg or more, the content of all nitrogen of 8,000 mg/kg or more, and a C/N ratio of 18 to 30.
摘要翻译：要解决的问题：提供一种具有高效石油污染净化的石油污染土壤的净化方法，并提供一种用于净化石油污染土壤的组合物，对石油污染物进行净化具有很高的效率。解决方案：净化方法石油污染土壤包括将石油污染土壤中所有碳含量（不包括石油含量）调整为10,000至60,000 mg / kg的石油污染土壤中C / N比为8至16的步骤，在石油污染的土壤中加入含石油降解菌的有机物质。用于净化石油污染土壤的组合物含有降解石油的细菌，其全部碳含量为150,000mg / kg以上，全氮含量为8,000mg / kg以上，C / N比为18〜 30。

2. 发明专利

JP2014113538A Modification method of contaminated soil 审中-公开
标题翻译：污染土壤改良方法
公开(公告)号：JP2014113538A
公开(公告)日：2014-06-26
申请号：JP2012268883
申请日：2012-12-08
申请人： Daiwa House Industry Co Ltd , 大和ハウス工業株式会社 , Ritsumeikan , 学校法人立命館
发明人： KAWAME YUSUKE , YOSHIDA KAZUO , KAWAGOE HIROKI , KUBOTA KENZO , KUBO MIKI , FUKUHARA YUKI
IPC分类号： B09C1/10 , B03C1/00 , B03C1/02 , B09C1/00 , B09C1/02 , B09C1/08 , C12N1/00
摘要： PROBLEM TO BE SOLVED: To provide a modification method of contaminated soil using granular or massive active carbon in modification of contaminated soil employing microorganisms, capable of preventing deterioration of decomposition performance of the microorganisms caused when combined with active carbon, allowing exertion of adsorption power of the active carbon to be revealed, while stably and continuously carrying out modification of a contaminant.SOLUTION: A modification method of contaminated soil utilizes: microorganisms present in contaminated soil and/or microorganisms newly mixed in the contaminated soil; granular and/or massive active carbon; and nutrient.
摘要翻译：要解决的问题：为了提供使用微生物对污染土壤进行改性的污染土壤的污染土壤的修改方法，能够防止当与活性炭组合时引起的微生物分解性能的劣化，从而允许使用活性炭活性炭，同时稳定和持续地进行污染物的改性。解决方案：污染土壤的改性方法是：在污染土壤中新近混合的污染土壤和/或微生物中存在的微生物; 颗粒状和/或大块活性炭; 和营养。

3. 发明专利

JP2014060966A System of detecting novel petroleum decomposing bacteria from environment 有权
标题翻译：从环境检测新石油分解细菌的系统
公开(公告)号：JP2014060966A
公开(公告)日：2014-04-10
申请号：JP2012208715
申请日：2012-09-21
申请人： Ritsumeikan , 学校法人立命館 , Daiwa House Industry Co Ltd , 大和ハウス工業株式会社
发明人： KUBO MIKI , FUKUHARA YUKI , YOSHIDA KAZUO , KAWAGOE HIROKI , KAWAME YUSUKE , KUBOTA KENZO
IPC分类号： C12Q1/68 , B09C1/10 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a primer set for detecting petroleum decomposing bacteria capable of quantifying petroleum decomposing bacteria specifically and with high sensitivity.SOLUTION: A primer set for detecting petroleum decomposing bacteria comprises (a) a degenerate primer comprising a DNA having continuous 12 bases or more in a particular base sequence, or a DNA having maximum of 25 bases including a particular base sequence, and (b) a degenerate primer comprising a DNA having continuous 12 bases or more in a particular base sequence, or a DNA having maximum of 25 bases including a particular base sequence.
摘要翻译：要解决的问题：提供一种用于检测能够特异性和高灵敏度地定量分解石油分解细菌的石油分解细菌的引物组。解决方案：用于检测石油分解细菌的引物组包括（a）简并引物，其包含具有连续12 或特定碱基序列的碱基或最多含有25个碱基的DNA，以及（b）包含特定碱基序列中具有连续12个碱基或更多个DNA的简并引物或具有最大值的DNA 包括特定的碱基序列的25个碱基。

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