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1. 发明专利

JP2009192547A Protein array for allelic variant and use thereof 审中-公开
标题翻译：蛋白质阵列用于不同的变体和用途
公开(公告)号：JP2009192547A
公开(公告)日：2009-08-27
申请号：JP2009132599
申请日：2009-06-01
申请人： Sense Proteomic Ltd , センスプロテオミックリミテッド
发明人： BOUTELL JONATHAN MARK , GODBER BENJAMIN LESLIE J , HART DARREN JAMES , BLACKBURN JONATHAN DAVID
IPC分类号： G01N33/53 , B01J19/00 , C12N15/09 , C12Q1/26 , C12Q1/68 , C40B30/04 , C40B40/02 , C40B40/06 , C40B40/10 , C40B50/06 , C40B60/14 , G01N33/68 , G01N37/00
CPC分类号： C40B30/04 , B01J19/0046 , B01J2219/00387 , B01J2219/005 , B01J2219/00527 , B01J2219/00574 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/0063 , B01J2219/00641 , B01J2219/00644 , B01J2219/00659 , B01J2219/00677 , B01J2219/00691 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/6845 , G01N2500/00
摘要： PROBLEM TO BE SOLVED: To provide protein arrays and their use to assay, in a parallel fashion, the protein products of highly homologous or related DNA coding sequences. SOLUTION: By highly homologous or related it is meant those DNA coding sequences which share a common sequence and which differ only by one or more naturally occurring mutations (such as single nucleotide polymorphisms, deletions or insertions), or those sequences which are considered to be haplotypes (a haplotype being a combination of variations or mutations on a chromosome, usually within the context of a particular gene). Such highly homologous or related DNA coding sequences are generally naturally occurring variants of the same gene. The array has multiple (for example, two or more) individual proteins deposited in a spatially defined pattern on a surface in a given form. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供蛋白质阵列及其用于以并行方式测定高度同源或相关DNA编码序列的蛋白质产物。解决方案：高度同源或相关的是指具有共同序列且仅与一个或多个天然存在的突变（例如单核苷酸多态性，缺失或插入）不同的那些DNA编码序列，或者是被认为是单倍型（单倍型是染色体上的变异或突变的组合，通常在特定基因的上下文中）。这种高度同源或相关的DNA编码序列通常是相同基因的天然存在的变体。该阵列具有以给定形式在表面上以空间限定的图案沉积的多个（例如，两个或更多个）单个蛋白质。版权所有（C）2009，JPO＆INPIT

2. 发明专利

JP2010156715A New use of protein encoded by ble gene and antibiotic from bleomycin family 审中-公开
标题翻译：由BLE基因编码的蛋白质的新使用和来自BLEOMYCIN家族的抗生素
公开(公告)号：JP2010156715A
公开(公告)日：2010-07-15
申请号：JP2010089809
申请日：2010-04-08
申请人： Sense Proteomic Ltd , センスプロテオミックリミテッド
发明人： BLACKBURN JONATHAN MICHAEL , GODBER BENJAMIN LESLIE J , HART DARREN JAMES , MCANDREWS MIKE
IPC分类号： G01N33/53 , A61K47/48 , C07K1/14 , C07K14/00 , C07K19/00 , G01N33/547 , G01N33/58 , G01N33/94 , G01N37/00
CPC分类号： G01N33/9446 , A61K47/6809 , C07K14/00 , G01N33/58 , G01N2415/00
摘要： PROBLEM TO BE SOLVED: To provide a method for using a protein complex including a "ble" fusion protein having a specific bonding characteristic.
SOLUTION: The protein complex can be bonded reversibly to an antibiotic from a bleomycin family, and the characteristic is utilized for various immobilization methods. The complex is used as a marker for protein expression and/or folding, or as a marker for affinity tagging. The invention also provides a probe including an array of an immobilized antibiotic from the bleomycin family, which acts as an analyte capture moiety. In another aspect, a purification media is provided, which includes an antibiotic of the bleomycin family as an analyte capture moiety. Further, a method is provided, for generating a soluble form of an insoluble protein by expressing the protein as a "ble" fusion protein and selecting in the presence of an antibiotic from the bleomycin family. In a further aspect, the "ble" protein is expressed as a fusion protein in a cell into which a labelled antibiotic of the bleomycin family is introduced, thereby allowing identification of the cellular localization of the protein.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种使用包含具有特定粘合特性的“可见”融合蛋白的蛋白质复合物的方法。解决方案：蛋白质复合物可以可逆地结合到博来霉素家族的抗生素，并且其特征用于各种固定化方法。该复合物用作蛋白质表达和/或折叠的标记，或作为亲和标记的标记物。本发明还提供了包含来自博来霉素家族的固定化抗生素阵列的探针，其作为分析物捕获部分。另一方面，提供了一种纯化介质，其包括作为分析物捕获部分的博来霉素家族的抗生素。此外，提供了一种通过将蛋白质表达为“可见”融合蛋白并在来自博来霉素家族的抗生素存在下选择来产生可溶形式的不溶性蛋白质的方法。在另一方面，“可溶”蛋白质在引入博来霉素家族的标记抗生素的细胞中表达为融合蛋白，从而允许鉴定蛋白质的细胞定位。版权所有（C）2010，JPO＆INPIT

3. 发明专利

JP2010068811A Enzyme array and assay 审中-公开
标题翻译： ENZYME阵列和测定
公开(公告)号：JP2010068811A
公开(公告)日：2010-04-02
申请号：JP2009296203
申请日：2009-12-25
申请人： Sense Proteomic Ltd , センスプロテオミックリミテッド
发明人： BLACKBURN JONATHAN MICHAEL , KOOPMAN JENS-OLIVER
IPC分类号： C12Q1/48 , C12N11/02
摘要： PROBLEM TO BE SOLVED: To provide an enzyme array and an assay for use using a mass spectrometer, concretely laser desorption/ionization such as a MALDI mass spectrometer or the like, though it is not limited.
SOLUTION: A method for determining an enzyme activity or effect which a test compound has to the enzyme activity using mass spectrometry includes: a process for providing a probe carrying an immobilized enzyme; a process for introducing a test compound by arbitrary selection; a process for introducing one or more reaction materials in sufficient time and a form for generating a reaction in the immobilized enzyme; a process for drying the probe; a process for performing a mass spectrometry to the probe; and a process for determining the enzyme activity or effect which the test compound has against the enzyme activity by detecting the presence and/or absence of one or more generated materials and/or one or more reaction materials. The method is a typical example in relation to a kinase assay. The array used in the method is also provided.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：尽管没有限制，提供使用质谱仪的酶阵列和测定法，具体地说是激光解吸/电离如MALDI质谱仪等。解决方案：使用质谱法测定测试化合物对酶活性的酶活性或作用的方法包括：提供携带固定化酶的探针的方法; 通过任意选择引入测试化合物的方法; 在足够的时间引入一种或多种反应物质的方法和在固定化酶中产生反应的形式; 用于干燥探针的方法; 用于对探针进行质谱分析的方法; 以及通过检测一种或多种所产生的材料和/或一种或多种反应材料的存在和/或不存在来确定测试化合物与酶活性相关的酶活性或作用的方法。该方法是与激酶测定相关的典型实例。还提供了该方法中使用的阵列。版权所有（C）2010，JPO＆INPIT

4. 发明专利

JP2009300449A Probe for mass spectrometry 审中-公开
标题翻译：探测质谱
公开(公告)号：JP2009300449A
公开(公告)日：2009-12-24
申请号：JP2009171590
申请日：2009-07-22
申请人： Sense Proteomic Ltd , センスプロテオミックリミテッド
发明人： KOOPMAN JENS-OLIVER , BLACKBURN JONATHAN M
IPC分类号： G01N27/64 , G01N27/68 , A61K47/48 , C07K14/00 , G01N27/62 , G01N33/483 , G01N33/53 , G01N33/94 , G01N37/00 , H01J49/04 , H01J49/16
CPC分类号： H01J49/0418 , A61K47/6809 , B82Y30/00 , C07K14/00 , G01N33/9446 , G01N2415/00 , Y10T436/24
摘要： PROBLEM TO BE SOLVED: To provide a protein microarray (not an array) to be analysed by laser desorption/ionisation mass spectrometry, more particularly matrix assisted laser desorption/ionization (MALDI). SOLUTION: A probe for use with a laser desorption/ionisation mass spectrometry meter includes a support having an electroconductive target surface thereon. The target surface includes a micro array having a plurality of discrete target areas presenting one or more analyte capture moieties. The support is a slide glass or MALDI target. COPYRIGHT: (C)2010,JPO&INPIT

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