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1. 发明公开

EP2229450A1 NOVEL GENE SMS 44 审中-公开
公开(公告)号：EP2229450A1
公开(公告)日：2010-09-22
申请号：EP09700851.0
申请日：2009-01-09
申请人： DSM IP Assets B.V.
发明人： CHEVREUX, Bastien , MOUNCEY, Nigel John
IPC分类号： C12P17/04 , C12P7/60 , C12N9/12 , C12N15/00
CPC分类号： C12N9/1223 , C12P7/60 , C12P17/04
摘要： The present invention relates to novel genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C) and/or 2-keto-L- gulonic acid (hereinafter also referred to as 2-KGA). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to modified proteins and polynucleotides encoding said modified proteins as well as to modified microorganisms, wherein the modification has a direct or indirect impact on yield, production, and/or efficiency of production of Vitamin C and/or 2-KGA in said microorganisms. Also included are processes of using the modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C and/or 2- KGA.
摘要翻译：本发明涉及编码参与L-抗坏血酸（以下也称为维生素C）和/或2-酮-L-古洛糖酸（以下也称为2-KGA）合成的蛋白质的新基因）。本发明的特征还在于包含新基因及其片段的全长多核苷酸序列的多核苷酸，由该多核苷酸及其片段编码的新多肽以及它们的功能等价物。本发明还涉及编码所述修饰蛋白的修饰蛋白和多核苷酸以及修饰的微生物，其中所述修饰对维生素C和/或2-KGA产生，产生和/或产生效率具有直接或间接影响在所述微生物中。还包括使用修饰的多核苷酸序列转化宿主微生物的过程。本发明还涉及基因工程微生物及其用于直接生产维生素C和/或2-KGA的用途。

2. 发明公开

EP2039774A1 Methods for the production of products in host cells 审中-公开
标题翻译：在Wirtszellen的Verfahren zur Herstellung von Produkten
公开(公告)号：EP2039774A1
公开(公告)日：2009-03-25
申请号：EP08015715.9
申请日：2002-04-04
申请人： Genencor International, Inc.
发明人： Dodge, Timothy, C. , Valle, Fernando
IPC分类号： C12P1/00 , C12P7/00 , C12P7/40 , C12P7/58 , C12P7/60 , C12N9/00 , C12N9/10 , C12N9/12 , C12N1/20 , C07H21/04
CPC分类号： C12N15/52 , C12P7/46 , C12P7/60 , C12P19/02
摘要： The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6 th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6 th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6 th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6 th carbon are provided.
摘要翻译：本发明提供了用于生产抗坏血酸中间体的方法和宿主细胞。本发明还提供了在多核苷酸中具有修饰的宿主细胞，其通过缺失在其第6个碳上磷酸化D-葡萄糖的内源性酶活性和/或已经缺失编码的多核苷酸的编码来解离分解代谢途径从氧化途径用于在其第6个碳上磷酸化D-葡萄糖酸盐的内源酶活性。这种宿主细胞用于生产产品，如抗坏血酸中间体。提供了具有灭活的酶活性的核酸和氨基酸序列，其在其第6碳上磷酸化D-葡萄糖，并且在其第6个碳上磷酸化D-葡萄糖酸盐的失活的酶活性。

3. 发明授权

EP1383903B1 METHODS FOR THE PRODUCTION OF ASCORBIC ACID INTERMEDIATES IN HOST CELLS 有权
标题翻译：过程在宿主细胞中产生ASCORBINSÄUREZWISCHENPRODUKTEN
公开(公告)号：EP1383903B1
公开(公告)日：2008-09-24
申请号：EP02741663.5
申请日：2002-04-04
申请人： GENENCOR INTERNATIONAL, INC.
发明人： DODGE, Timothy, C. , VALLE, Fernando
IPC分类号： C12P1/00 , C12P7/00 , C12P7/40 , C12P7/58 , C12P7/60 , C12N9/00 , C12N9/10 , C12N9/12 , C12N1/20 , C07H21/04
CPC分类号： C12N15/52 , C12P7/46 , C12P7/60 , C12P19/02

4. 发明公开

EP1649030A4 METABOLICALLY ENGINEERED BACTERIAL STRAINS HAVING ENHANCED 2-KETO-D-GLUCONATE ACCUMULATION 失效转让
标题翻译：代谢肉豆蔻精油甜味剂2-KETO-D-GLUCONAT-ANREICHERUNG
公开(公告)号：EP1649030A4
公开(公告)日：2007-03-14
申请号：EP04779304
申请日：2004-07-27
申请人： GENENCOR INT
发明人： DODGE TIMOTHY C , VALLE FERNANDO , RASHID M HARUNUR
IPC分类号： C12P1/00 , C07H21/04 , C12N20060101 , C12N1/21 , C12N9/04 , C12N15/74 , C12P1/04 , C12P7/00 , C12P7/26 , C12P7/58 , C12P7/60 , C12P19/02
CPC分类号： C12P7/60 , C12N9/0006 , C12P19/02

5. 发明授权

EP1543136B1 MICROBIAL PRODUCTION OF VITAMIN C 有权
标题翻译：维生素C对微生物生产
公开(公告)号：EP1543136B1
公开(公告)日：2006-12-27
申请号：EP03750591.4
申请日：2003-09-22
申请人： DSM IP Assets B.V.
发明人： HOSHINO, Tatsuo , SUGISAWA, Teruhide
IPC分类号： C12P17/04 , C12P7/60
CPC分类号： C12P7/60 , C12P17/04
摘要： The present invention provides a process for the production of vitamin C from different substrates like D-sorbitol, L-sorbose, L-sorbosone or L-gulose using a microorganism selected from the group consisting of Gluconobacter oxydans DSM 4025 (FERM BP-3812), a microorganism belonging to the genus Gluconobacter and having identifying characteristics of G. oxydans DSM 4025 (FERM BP-3812) and mutants thereof.

6. 发明公开

EP1525300A4 METHODS FOR PRODUCING ETHANOL FROM CARBON SUBSTRATES 审中-公开
标题翻译：生产乙醇的方法从碳底物，
公开(公告)号：EP1525300A4
公开(公告)日：2006-03-15
申请号：EP03713381
申请日：2003-02-06
申请人： GENENCOR INT
发明人： LANTERO ORESTE J JR
IPC分类号： C12P7/08 , C12P7/06 , C12P7/10 , C12P7/18 , C12P7/20 , C12P7/44 , C12P7/56 , C12P7/58 , C12P7/60 , C12R1/66 , C12N1/20 , C12N15/00 , C12P7/16
CPC分类号： C12P7/06 , C12P7/10 , C12P7/18 , C12P7/20 , C12P7/44 , C12P7/56 , C12P7/58 , C12P7/60 , Y02E50/16 , Y02E50/17
摘要： The present invention provides means for the production of desired end-products of in vitro and/or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and/or liquefaction of the substrate. In particularly preferred embodiments, the present invention provides means for the production of ethanol.

7. 发明公开

EP1383903A2 METHODS FOR THE PRODUCTION OF PRODUCTS IN HOST CELLS 有权
标题翻译：过程在宿主细胞中产生ASCORBINSÄUREZWISCHENPRODUKTEN
公开(公告)号：EP1383903A2
公开(公告)日：2004-01-28
申请号：EP02741663.5
申请日：2002-04-04
申请人： GENENCOR INTERNATIONAL, INC.
发明人： DODGE, Timothy, C. , VALLE, Fernando
IPC分类号： C12P1/00 , C12P7/00 , C12P7/40 , C12P7/58 , C12P7/60 , C12N9/00 , C12N9/10 , C12N9/12 , C12N1/20 , C07H21/04
CPC分类号： C12N15/52 , C12P7/46 , C12P7/60 , C12P19/02
摘要： The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided.

8. 发明授权

EP0606621B1 Alcohol/aldehyde dehydrogenase 失效
标题翻译：乙醇/乙醛脱氢酶
公开(公告)号：EP0606621B1
公开(公告)日：2002-09-25
申请号：EP93120511.6
申请日：1993-12-20
申请人： F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft
发明人： Akira, Asakura , Tatsuo, Hoshino
IPC分类号： C12N9/02 , C12N9/04 , C12P7/60 , C12P7/24
CPC分类号： C12N9/0008 , C12N9/0006 , C12P7/60 , C12Y101/01001 , Y10S435/822

9. 发明公开

EP1183385A1 METHODS AND MATERIALS FOR THE SYNTHESIS OF ORGANIC PRODUCTS 无效转让
标题翻译：方法和材料合成有机产品
公开(公告)号：EP1183385A1
公开(公告)日：2002-03-06
申请号：EP00932649.7
申请日：2000-05-19
申请人： Cargill Dow LLC
发明人： RAJGARHIA,Vineet , HATZIMANIKATIS, Vassily , OLSON, Stacey , CARLSON, Ting Liu , STARR, John N. , KOLSTAD, Jeffrey J.
IPC分类号： C12P7/56 , C12P7/40 , C12P7/48 , C12P7/60 , C12P19/02 , C12N15/53 , C12N15/81 , C12N9/04 , C12N1/19
CPC分类号： C12N1/16 , C12P7/40 , C12P7/48 , C12P7/56 , C12P7/60 , Y02E50/17
摘要： The invention provides methods and materials related to the production of organic products. Specifically, the invention provides various recombinant yeast cells, methods for culturing yeast cells, methods for making yeast cells, nucleic acid constructs, and methods and materials for producing various organic products.

10. 发明公开

EP1164192A1 SORBITOL DEHYDROGENASE, GENE ENCODING THE SAME AND USE THEREOF 审中-公开
标题翻译： SORBITOL-DEHYDROGENASE，DAFüRKODIERENDES GEN UND DEREN VERWENDUNG
公开(公告)号：EP1164192A1
公开(公告)日：2001-12-19
申请号：EP00909679.3
申请日：2000-03-16
申请人： FUJISAWA PHARMACEUTICAL CO., LTD.
发明人： SHIBATA, Takashi , ICHIKAWA, Chiyo , MATSUURA, Mitsutaka , NOGUCHI, Yuji , SAITO, Yoshimasa , YAMASHITA, Michio , TAKATA, Yoko
IPC分类号： C12N15/53 , C12N9/04 , C12N1/21 , C12P19/02 , C12P7/60
CPC分类号： C12N9/0006 , C12P7/60 , C12P19/02
摘要： The present invention provide a gene encoding D-sorbitol dehydrogenase (SLDH), a method for producing SLDH by culture of a host cell transformed with a vector expressing this gene and a method for producing L-sorbose or 2-keto-L-gulonic acid (2KLGA) using the culture. 2KLGA is an important intermediate for the production of L-ascorbic acid. Therefore, the present invention also provides a method for producing L-ascorbic acid from the 2KLGA obtained by the above-mentioned method.
摘要翻译：本发明提供编码D-山梨糖醇脱氢酶（SLDH）的基因，通过用表达该基因的载体转化的宿主细胞的培养来生产SLDH的方法以及L-山梨糖或2-酮-L-古洛糖酸（2KLGA）。 2KLGA是生产L-抗坏血酸的重要中间体。因此，本发明还提供了通过上述方法得到的2KLGA生产L-抗坏血酸的方法。

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