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1. 发明公开

EP2193369A1 A HIGH THROUGHPUT TEST METHOD FOR EVALUATION OF BIOCIDES AGAINST ANAEROBIC MICROORGANISMS 审中-公开
标题翻译：高吞吐量测试方法进行评估杀生物AGAINST厌氧微生物
公开(公告)号：EP2193369A1
公开(公告)日：2010-06-09
申请号：EP08799370.5
申请日：2008-09-10
申请人： Dow Global Technologies Inc.
发明人： YIN, Bei
IPC分类号： G01N33/50
CPC分类号： C12Q1/18 , C12Q1/12 , G01N2333/315 , G01N2333/33 , G01N2333/335 , G01N2333/36
摘要： Provided is a high-throughput method for determining the biocidal efficacy of biocidal agents against anaerobic organisms. The method includes the steps of: providing one or more anaerobe samples in a first set of multiple receptacles accessible to multi- channel pipettes; providing one or more biocidal samples at known concentration(s) in a second set of multiple receptacles accessible to multi-channel pipettes; forming mixtures of the one or more biocidal samples and the anaerobe samples via a multi-channel pipette; incubating the mixtures so as to allow reaction between the biocidal samples and the anaerobe samples; determining each of the one or more biocidal samples' killing (biocidal) effectiveness against the anaerobes at selected time interval(s), wherein each step, except for the step of providing one or more biocidal samples, is conducted under anaerobic conditions.

2. 发明公开

EP0831151A1 Verfahren und Vorrichtung zur Messung der Nitrifikationsleistung von Belebtschlamm 失效
公开(公告)号：EP0831151A1
公开(公告)日：1998-03-25
申请号：EP97250278.5
申请日：1997-09-17
申请人： LAR Analytik und Umweltmesstechnik GmbH
发明人： Pilz, Ulrich
IPC分类号： C12Q1/00 , G01N33/48 , C02F3/12
CPC分类号： C02F3/006 , C02F2209/02 , C02F2209/22 , C02F2209/225 , C02F2209/24 , C02F2209/245 , C02F2209/40 , C12Q1/12 , G01N33/1806 , G01N33/182 , Y10T436/17 , Y10T436/173076 , Y10T436/20 , Y10T436/209163 , Y10T436/25875
摘要： Verfahren zur Bestimmung der Nitrifikationsleistung von Belebtschlamm in einer wäßrigen Lösung, insbesondere mit Kohlenwasserstoffen belastetem Wasser, mit den Schritten:

Zuführen einer jeweils vorbestimmten oder meßtechnisch erfaßten Menge des Belebtschlamms, der wäßrigen Lösung und von Luft oder Sauerstoff zu einem ersten und einem zweiten Reaktionsgefäß (101,102),
gleichzeitiges Zuführen eines Nitrifkationsinhibitors nur zu dem zweiten Reaktionsgefäß (102),
Ausführen einer Veratmungsreaktion in dem ersten und zweiten Reaktionsgefäß (101,102) für eine vorbestimmte Zeitdauer oder kontinuierlich mit einer pro Zeiteinheit bestimmten Menge an Belebtschlamm, wäßriger Lösung und Luft oder Sauerstoff,
Messung des Sauerstoffgehaltes über der wäßrigen Lösung im ersten und zweiten Reaktionsgefäß (101,102) nach gleicher Reaktionszeit in beiden Reaktionsgefäßen (101,102) oder kontinuierlich in einem aus dem jeweiligen Reaktionsgefäß entweichenden Abluftstrom,
vergleichende Auswertung der am ersten und zweiten Reaktionsgefäß (101,102) ermittelten Sauerstoffgehalts-Meßwerte unter Berücksichtigung der Mengen der Einsatzstoffe und unter Einschluß einer Differenzbildung aus den Sauerstoffgehalts-Meßwerten oder aus den Werten einer jeweils von diesen abgeleiteten Größe.
摘要翻译：该方法确定水溶液中活性污泥硝化负荷，特别是含烃。通过测量，水溶液（104）和空气（110A）或氧气将一定数量的活性污泥（106）加入到第一（101）和第二（102）反应容器中。同时，硝化抑制剂（107）仅被添加到第二容器（102）中。氧消耗反应进行一段时间; 或者连续地测量（110,111）材料的传送速率。在间隔之后，在容器中的溶液中或在连续进行的流出空气中测量氧（114.1,114.2）。比较氧含量值与存在的初始物质相关（119），以确定结果。还要求的是执行该程序的相应设备。

3. 发明公开

EP0318245A3 Means and method for enhancing nucleic acid hybridization 有权转让
标题翻译：用于增强核酸杂交的方法和方法
公开(公告)号：EP0318245A3
公开(公告)日：1990-09-12
申请号：EP88311036.3
申请日：1988-11-22
申请人： GEN-PROBE INCORPORATED
发明人： Hogan, James John , Milliman, Curt Lawrence
IPC分类号： C12Q1/68
CPC分类号： C12Q1/12 , C12Q1/6832 , C12Q1/689 , Y10S435/81
摘要： The binding of a nucleic acid probe with its complementary sequence in a targeted, single stranded nucleic acid is affected by the secondary and tertiary structure of the target nucleic acid. The rate and extent of hybridization of the probe with the targeted nucleic acid can be increased by the use of "helper" oligonucleotides. Helper oligonucleotides are selected to bind to the target nucleic acid and impose a different secondary and tertiary structure on the target to facilitate the binding of the probe to the target. The resulting hybrid of probe and target nucleic acid also exhibits a higher T m than the hybrid which results from addition of the probe alone.

4. 发明公开

EP0173428A1 Processes and materials for carrying out microchemical and microbiological tests 有权转让
标题翻译：进行微量化学和微生物检测的工艺和材料
公开(公告)号：EP0173428A1
公开(公告)日：1986-03-05
申请号：EP85304485.7
申请日：1985-06-24
申请人： RADIOMETER CORPORATE DEVELOPMENT LIMITED
发明人： Sands, Thomas John
IPC分类号： C12Q1/12 , G01N31/22 , G01N33/52
CPC分类号： G01N31/227 , C12Q1/12 , G01N33/52 , Y10S435/81 , Y10S436/903 , Y10T436/173076
摘要： A microbiological culture test process for detecting the presence of nitrate-reducing microorganism in an inoculum sample, which comprises

(a) culturing a microorganism to be tested in the presence of adequately nitrite-free nitrate, for a period sufficient to allow reduction of nitrate by any microorganism (if present) that has the capacity to reduce nitrate to nitrite, (e.g. 1-6 hours);
(b) exposing the culture medium after culture to (diazotising) acid conditions in the presence of an amino-containing fluorophor thereby to cause diazotisation of the fluorophor to the extent of any nitrite present in the medium to form a diazonium derivative (or further reaction product thereof) which is substantially less fluorescent (or fluoresces at a substantially different wavelength) than said amino-containing fluorophor, and
(c) assessing the fluorescence of the culture medium as treated by step (b), thereby to show a reduction in fluorescence in the presence of a nitrate-reducing microorganism.
摘要翻译：一种用于检测接种物样品中硝酸盐还原微生物的存在的微生物培养试验方法，该方法包括（a）在充足的不含亚硝酸盐的硝酸盐的存在下培养待测微生物足够的时间以允许通过任何能够将硝酸盐还原成亚硝酸盐（如1-6小时）的微生物（如果存在的话）; （b）在含有氨基的荧光团存在下，将培养后的培养基暴露于（重氮化）酸性条件下，从而使荧光团重氮化至存在于培养基中的任何亚硝酸盐的程度以形成重氮衍生物（或进一步反应（c）鉴定经步骤（b）处理的培养基的荧光，从而显示出荧光的降低（例如其产物），所述荧光显着低于所述含氨基的荧光团的荧光（或在实质上不同的波长发荧光）在存在硝酸盐还原微生物的情况下。

5. 发明公开

EP0025467A1 Chromogenic chemical substrates for identification of microbial colonies 失效
标题翻译：化学染色体基质zur识别冯Mikrobenkolonien。
公开(公告)号：EP0025467A1
公开(公告)日：1981-03-25
申请号：EP79400638.7
申请日：1979-09-12
申请人： Rambach, Alain , Société TECHNOGRAM
发明人： Rambach, Alain
IPC分类号： C12Q1/04 , C12Q1/12
CPC分类号： C12Q1/10 , C12Q1/12 , C12Q2334/00 , G01N2333/245
摘要： Chromogenic substrate member for identifying Escherichia coli bacteria. It comprises a growing medium for said bacteria and container means (1) for the growing medium (4) and a liquid sample to be tested. The container means also contains a chromogenic reagent compound consisting in 8-hydroxyquinoline-β-D glucuronide and an activator compound consisting in X-glucuronide where X is selected from the group consisting in alkyl, aryl or indoxyl radical, the halogeno substitutes and the nitro substitutes thereof.
摘要翻译：用于鉴定大肠杆菌细菌的显色底物。其包括用于所述细菌的生长培养基和用于生长培养基（4）的容器装置（1）和待测试的液体样品。容器装置还含有由8-羟基喹啉-β-D-葡糖苷酸组成的显色试剂化合物和由X-葡萄糖醛酸苷组成的活化剂化合物，其中X选自烷基，芳基或吲哚基，卤素取代基和硝基替代。

6. 发明公开

EP0005512A2 A serological method for determining the presence of Neisseria gonorrhoeae antibodies in human serum, a species-specific heat stable antigen to be used in this method and a method for the preparation of that antigen 有权
标题翻译：人血清中检测淋病奈瑟氏球菌的抗体的血清学方法中，对施加的物种比热稳定抗原和用于制备该抗原的方法。
公开(公告)号：EP0005512A2
公开(公告)日：1979-11-28
申请号：EP79101430.1
申请日：1979-05-10
申请人： Research Corporation
发明人： Gaafar, Hassan A.
IPC分类号： G01N33/54 , C07G7/00 , C12Q1/04 , C12R1/36
CPC分类号： G01N33/571 , A61K39/095 , C12Q1/12 , Y10S435/961 , Y10S530/806 , Y10S530/825
摘要： Serological method for determining the presence of Neisseria gonorrhoeae antibodies in human sera and products utilized in such test.

7. 发明公开

EP3194373A4 DIAGNOSTIC SYSTEM AND PROCESS FOR RAPID BACTERIAL INFECTION DIAGNOSIS 有权
公开(公告)号：EP3194373A4
公开(公告)日：2018-04-04
申请号：EP15841956
申请日：2015-09-16
申请人： UNIV NORTHEASTERN
发明人： GOLUCH EDGAR D , SISMAET HUNTER J , WEBSTER THADDAEUS A
IPC分类号： C12Q1/02 , A61K31/496 , A61K38/12 , B01L3/00 , C12M1/34 , C12Q1/04 , C12Q1/12 , C12Q1/18 , C12Q1/22 , G01N27/327 , G01N27/48 , G01N33/487
CPC分类号： C12Q1/12 , A61K31/496 , A61K38/12 , B01L3/5027 , B01L3/502715 , B01L2300/02 , B01L2300/0636 , B01L2300/0645 , C12M1/34 , C12Q1/02 , C12Q1/04 , C12Q1/18 , C12Q1/22 , G01N27/327 , G01N27/48 , G01N33/48707 , G01N33/48735 , G01N2333/21
摘要： Methods and devices for monitoring the viability of a biofilm comprising Pseudomonas Aeruginosa bacteria are provided by detecting pyocyanin. The invention relates to electrochemical methods and devices that offer a simple and inexpensive alternative for immediate identification of bacterial infection due to the presence of Pseudomonas aeruginosa. In some embodiments, an inexpensive, disposable electrochemical sensor can be used to rapidly screen for the presence of P. aeruginosa in clinical wound effluent samples.

8. 发明公开

EP3145519A1 SYSTEMS AND METHODS FOR STORAGE AND DELIVERY OF AMMONIA OXIDIZING BACTERIA 有权转让
标题翻译：系统和方法进行存储和分发氨细菌的
公开(公告)号：EP3145519A1
公开(公告)日：2017-03-29
申请号：EP15729283.0
申请日：2015-05-21
申请人： Aobiome LLC
发明人： HEYWOOD, James , JAMAS, Spiros , WHITLOCK, David, R. , WEISS, Larry
IPC分类号： A61K35/741
CPC分类号： A61K35/74 , A61J1/20 , A61J1/2003 , A61J1/202 , A61J1/2089 , A61K8/645 , A61K8/99 , A61K35/741 , B65D81/32 , C12M41/00 , C12Q1/12
摘要： This disclosure provides, inter alia, systems and methods for storage and delivery of ammonia oxidizing bacteria, and preparations comprising ammonia oxidizing bacteria. The ammonia oxidizing bacteria may be provided in a container or a kit, with one or more other components that may enhance delivery. The systems and methods herein may be used, for instance, to treat diseases associated with low nitrite levels, skin diseases, and diseases caused by pathogenic bacteria.
摘要翻译：本发明提供，除其他外，系统和用于存储和氨氧化细菌的递送方法和制剂包括氨氧化细菌。氨氧化细菌可以在容器中或试剂盒中提供，其中一个或多个其他部件也可提高递送。该系统和方法可用于在，例如，治疗与低亚硝酸盐水平，皮肤疾病，和由致病细菌引起的疾病相关的疾病。

9. 发明公开

EP2308993A1 Bacteria analysis apparatus, bacteria analysis method and computer program product 审中-公开
标题翻译： Bakterienanalysegerät，Bakterienanalyseverfahren和Computerprogrammprodukt
公开(公告)号：EP2308993A1
公开(公告)日：2011-04-13
申请号：EP10179352.9
申请日：2010-09-24
申请人： SYSMEX CORPORATION
发明人： Wada, Atsushi
IPC分类号： C12Q1/04 , C12Q1/12
CPC分类号： C12Q1/04 , C12Q1/10 , C12Q1/12 , G01N2333/936 , G01N2333/96419
摘要： A bacteria analysis apparatus comprising: a specimen preparation section for preparing a first measurement specimen from a sample by using a first enzyme; a detecting section for detecting bacteria included in the first measurement specimen; and an information processing section for outputting information for supporting determination or kind of bacteria included in the sample on the basis of the detection result of the first measurement specimen. A method and a computer program product is also disclosed.
摘要翻译：一种细菌分析装置，其特征在于，包括：样本制备部，其通过使用第一种酶从样品制备第一测定试样; 用于检测包含在第一测量样本中的细菌的检测部分; 以及信息处理部分，用于基于第一测量样本的检测结果输出用于支持样品中包含的细菌的确定或种类的信息。还公开了一种方法和计算机程序产品。

10. 发明公开

EP0160240A2 Test piece for detecting nitrite 有权
标题翻译： Teststückzum Nachweis von Nitrit。
公开(公告)号：EP0160240A2
公开(公告)日：1985-11-06
申请号：EP85104305.9
申请日：1985-04-10
申请人： TERUMO KABUSHIKI KAISHA trading as TERUMO CORPORATION
发明人： Kaminagayoshi, Satoshi
IPC分类号： G01N33/84 , C12Q1/12 , G01N33/52
CPC分类号： G01N31/227 , C12Q1/12 , G01N33/84 , Y02A50/451 , Y02A50/59
摘要： In a test piece for detecting a nitrite by virtue of the diazo coupling reaction, which test piece has supported on a substrate an effective amount of at least one oxidizing agent selected from the group consisting of M'XO, M'XO 3 , M'XO 4 , M' 3 H 2 XO 6 , wherin M' denotes an atom capable of being converted into a monovalent cation and X denotes a halogen atom, ethylenediamine tetraacetic acid-metals, cuprous chloride, copper sulfate, copper acetate, mercury acetate and bismuth acetate in corporation with a diazotizing agent and a coupling agent.
摘要翻译：在用于通过重氮偶联反应检测亚硝酸盐的测试片中，该测试片已经支撑在基底上，有效量的至少一种氧化剂选自M XO，M XO 3 ，M X 4，M 3 H 2 XO 6，Wherin M表示能够转化成一价阳离子的原子，X表示卤素原子，乙二胺四乙酸金属，氯化亚铜，硫酸铜，乙酸铜，乙酸汞和乙酸铋与重氮化剂和偶联剂共同使用。

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