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1. 发明授权

EP2970990B1 COMPOSITIONS AND METHODS FOR THE PRODUCTION AND DELIVERY OF RNA 有权
公开(公告)号：EP2970990B1
公开(公告)日：2018-07-18
申请号：EP14729497.9
申请日：2014-03-13
申请人： Monsanto Technology LLC
发明人： BAUM, James A. , CHRISTIAN, Allen T. , EVDOKIMOV, Artem , MOSHIRI, Farhad , WEAVER, Lisa Marie , ZHANG, Haitao
IPC分类号： C12N15/67
CPC分类号： A01N63/02 , A01H3/00 , A01N63/00 , C12N9/22 , C12N15/67 , C12N15/72
摘要： Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

2. 发明公开

EP2742032A1 GEM-DIFLUORINATED C-ISOPROPYLGALACTOSIDE DERIVATES 有权
标题翻译：创业板二氟碳ISOPROPYLGALACTOSID衍生物
公开(公告)号：EP2742032A1
公开(公告)日：2014-06-18
申请号：EP12751464.4
申请日：2012-08-08
申请人： Tfchem
发明人： DELIENCOURT-GODEFROY, Géraldine , FILLON, Hyacinthe
IPC分类号： C07D309/10 , C12N15/72
CPC分类号： C12N15/635 , C07D309/10 , C07H7/02 , C12N15/72 , C12P21/00
摘要： The present invention concerns compounds of the following formula (I) or a pharmaceutically acceptable salt thereof, a stereoisomer or a mixture of stereoisomers in any proportion, in particular a mixture of enantiomers, and particularly a racemate mixture, in which R represents H, OH or OR
19 , as well as processes for preparing these compounds and their use as inducer for the transcription of genes under control of the
lac promoter.

3. 发明授权

EP1576094B1 METHODS OF CREATING MODIFIED PROMOTERS RESULTING IN VARYING LEVELS OF GENE EXPRESSION 有权
标题翻译：用于生产以领先的改性发起人变基因表达水平
公开(公告)号：EP1576094B1
公开(公告)日：2011-09-28
申请号：EP03724099.1
申请日：2003-04-18
申请人： Danisco US Inc.
发明人： CERVIN, Marguerite, A. , VALLE, Fernando
IPC分类号： C12Q1/68 , C12N1/20 , C12N15/00 , C07H21/02 , C07H21/04
CPC分类号： C12N15/1082 , C12N15/63 , C12N15/70 , C12N15/71 , C12N15/72 , C40B30/06 , C40B40/02 , C40B50/06
摘要： The present invention relates to a method of creating promoter cassettes that include modified precursor promoters and transforming a population of bacterial host cells with a promoter library comprising the promoter cassettes resulting in bacterial clones having a range of expression levels of a gene of interest. The invention further relates to selecting a transformed bacterial host cell which has an optimum level of gene expression.

4. 发明公开

EP1859043A1 PHAGE-DERIVED VECTORS AND METHODS FOR PROTEIN EXPRESSION 审中-公开
标题翻译：噬菌体的载体和蛋白表达方法
公开(公告)号：EP1859043A1
公开(公告)日：2007-11-28
申请号：EP06735340.9
申请日：2006-02-17
申请人： Tabaczynski, David
发明人： Tabaczynski, David
IPC分类号： C12N15/70 , C12P21/06
CPC分类号： C12N15/70 , C12N15/72 , C12P21/02
摘要： A novel system, including vectors, phage particles, host cells and methods of use, for expressing one or more polypeptides of interest in prokaryotes is described.

5. 发明公开

EP1620559A1 MINICIRCLE VECTOR PRODUCTION 有权转让
标题翻译：生产MINI CIRCLE载体
公开(公告)号：EP1620559A1
公开(公告)日：2006-02-01
申请号：EP04730974.5
申请日：2004-05-04
申请人： MAYRHOFER, Peter
发明人： MAYRHOFER, Peter , JECHLINGER, Wolfgang , LUBITZ, Werner
IPC分类号： C12N15/63
CPC分类号： C12N15/1003 , C12N15/64 , C12N15/70 , C12N15/72 , C12N15/79
摘要： A plasmid is provided comprising comprising the following functional units: a prokaryotic origin of replication, a marker sequence, two specific recombinase recognition sequences and a multiple cloning site, whereby it comprises a gene coding for a sequence specific recombinase, whereby the units are arranged on the plasmid in such a way that the plasmid is divided into a miniplasmid and a minicircle upon expression of the sequence specific recombinase, said miniplasmid comprising the prokaryotic origin of replication, the marker sequence and the gene for the sequence specific recombinase and said minicircle comprising the multiple cloning site.

6. 发明公开

EP1451223A2 EXPRESSION CONTROL USING VARIABLE INTERGENIC SEQUENCES 有权转让
标题翻译：表达控制使用可变的干扰序列
公开(公告)号：EP1451223A2
公开(公告)日：2004-09-01
申请号：EP02785614.5
申请日：2002-12-05
申请人： Celltech R & D Limited
发明人： POPPLEWELL, Andrew, George
IPC分类号： C07K16/00 , C12N15/70 , C12N1/21
CPC分类号： C07K16/2875 , C07K16/00 , C07K2317/55 , C12N15/72
摘要： The present invention relates to a method of production of antibodies wherein the heavy and light chains of a particular antibody molecule are encoded by the DNA present in a dicistronic message in which the two cistrons are linked by an optimised intergenic sequence.
摘要翻译：本发明涉及生产抗体的方法，其中特定抗体分子的重链和轻链由存在于双顺反子信息中的DNA编码，其中两个顺反子通过优化的基因间序列连接。

7. 发明公开

EP1033408A4 COLD-INDUCIBLE EXPRESSION VECTOR 有权
标题翻译： BY冷诱导表达载体
公开(公告)号：EP1033408A4
公开(公告)日：2004-08-25
申请号：EP98953080
申请日：1998-11-17
申请人： TAKARA BIO INC
发明人： TAKAYAMA MASANORI , NOMURA YOSHIKO , KATO IKUNOSHIN
IPC分类号： C07K14/245 , C12N9/24 , C12N15/00 , C12N15/63 , C12N15/67 , C12N15/70 , C12N15/72 , C12P21/00
CPC分类号： C12N15/72 , C07K14/245 , C12N9/2471 , C12N15/00 , C12N15/635 , C12N15/67 , C12N15/70 , C12Y302/01023
摘要： A vector having the following elements: (1) a promoter which functions in a host to be used, (2) a regulatory region for regulating the function of the promoter (1), and (3) a region coding for a 5'-untranslated region derived from cold shock protein gene mRNA, or a region coding for a region formed by subjecting the untranslated region to substitution, deletion, insertion, or addition of at least one base.

8. 发明公开

EP1005559A4 IMPROVED CLONING VECTOR CONTAINING MARKER INACTIVATION SYSTEM 失效
标题翻译：改进的克隆载体MARKERINAKTIVIERUNGSSYSTEM
公开(公告)号：EP1005559A4
公开(公告)日：2001-04-11
申请号：EP98920107
申请日：1998-05-01
申请人： SLILATY STEVE N , LEBEL SUZANNE
发明人： SLILATY STEVE N , LEBEL SUZANNE
IPC分类号： C12N15/09 , C12N15/63 , C12N15/65 , C12N15/72
CPC分类号： C12N15/72
摘要： Provided are lacZ alpha gene fragments which have been modified to introduce multiple restriction enzyme sites. Vectors according to the present invention include at least one promoter operatively linked to a DNA sequence encoding lacZ alpha ( alpha -peptide); multiple cloning sites cleavable by distinct restriction enzymes which have been introduced within a lacZ coding sequence from and including the codon for amino acid (8), and in the lacZ coding sequence downstream of the codon for amino acid (8), in forming the modified lacZ alpha coding sequence; and a replicon. The vector may further comprise one or more additional features useful for protein expression and other molecular manipulations. Also provided are methods of using the vectors wherein a DNA molelcule is cloned into at least one restriction enzyme site in the modified lacZ alpha coding sequence, in forming recombinant vectors; introducing the recombinant vectors into competent host cells; growing the host cells in the presence of a chromogenic substrate cleavable by beta -galactosidase; and screening for indicia of lac operon marker inactivation. The high accuracy of color selection afforded by the modified lacZ alpha coding sequence of the present invention provides a first opportunity at performing gap-free shotgun sequencing and development of ordered genomic libraries.

9. 发明公开

EP1019487A1 EXPRESSION CONTROL SEQUENCES 审中-公开
标题翻译： EXPRESSIONSKONTROLLSEQUENZEN
公开(公告)号：EP1019487A1
公开(公告)日：2000-07-19
申请号：EP98949495.0
申请日：1998-09-28
申请人： Human Genome Sciences
发明人： GENTZ, Reiner, L. , COLEMAN, Timothy, A.
IPC分类号： C12N1/00 , C12N5/00 , C12N15/11 , C12N15/63 , C12N15/70 , C12N15/72 , C12P21/02
CPC分类号： C12N15/72
摘要： Expression control sequences are provided for the expression of proteins from a gene of interest. The gene may express viral, prokaryotic, or eukaryotic proteins. These control sequences are produced by combining phage promoter and operator/repressor systems. Expression vectors containing such expression control sequences, microorganisms transformed with such expression vectors and methods for producing viral, prokaryotic, and eukaryotic proteins using the expression control sequences, expression vectors and transformed microorganisms are also provided.

10. 发明公开

EP0610517A4 DNA CODING FOR DECARBAMYLASE IMPROVED IN THERMOSTABILITY AND USE THEREOF. 失效
标题翻译：用于改善热稳定性的丹磺酸的DNA编码及其用途。
公开(公告)号：EP0610517A4
公开(公告)日：1998-06-10
申请号：EP94906767
申请日：1993-08-05
申请人： KANEGAFUCHI CHEMICAL IND
发明人： IKENAKA YASUHIRO - - KAWASAKI- , NANBA HIROKAZU KOUNRYO - OKIHA , TAKANO MASAYUKI - - HIGASHIFUT , YAJIMA KAZUYOSHI - - NAKAOCHIA , YAMADA YUKIO - MINORI , TAKAHASHI SATOMI - - SHINWADAI
IPC分类号： C12N9/80 , C12N15/55 , C12N15/72 , C12P13/06 , C12P13/08 , C12P13/22 , C12N1/21
CPC分类号： C12N15/72 , C12N9/80 , C12P13/06 , C12P13/08 , C12P13/22
摘要： A DNA fragment coding for a decarbamylase protein improved in thermostability as the result of replacement of at least one base of a DNA fragment coding for a decarbamylase protein originating in a microbe with another base and the resultant replacement of at least one of the corresponding amino acids; a process for producing the fragment; a vector containing the fragment; a transformant produced with the vector; a decarbamylase improved in thermostability; and a process for producing the decarbamylase. Also disclosed is a process for producing a D-(alpha)-amino acid which comprises decarbamoylating an N-carbamoyl-D-(alpha)-amino acid with a decarbamylase having a thermostable temperature of 65 or above in an aqueous medium.

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