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    • 6. 发明公开
    • ANALYTICAL IMMUNOSENSOR DEVICE AND METHOD FOR CONSTRUCTING SAME
    • VERFAHREN ZER HERSTELLUNG DAVON分析仪器
    • EP2960650A1
    • 2015-12-30
    • EP14754002.5
    • 2014-02-19
    • Universidad de Alcalá
    • DOMÍNGUEZ CAÑAS, ElenaNARVÁEZ GARCÍA, ArantzazuJIMÉNEZ CENTELLES, Javier
    • G01N33/53
    • G01N33/54386B05D1/007B05D1/18G01N33/54393
    • The invention relates to an analytical immunosensor device and to a method for the constructing same, based on the sequential deposition of self-assembled monolayers of polymers and affinity elements, applicable for the detection and quantification of any target antigen or analyte in a liquid sample, and allowing the implementation of the "quasi-reagentless" or "reagentless" displacement assay, the minimisation of the non-specific adsorption and, as a result, the reduction of the detection limit. Said device is designed on the basis of a sensor surface covered with polymer monolayers (redox or non-redox) on which are deposited sub-monolayers of affinity elements, in order to subsequently carry out the affinity reaction with an antigen, pseudo-antigen or a hapten marked with a protein, preferably an enzyme (redox or non-redox) or a nanoparticle, allowing said method for constructing the device to develop "a la carte" immunosensors, with an electrochemical, optical and/or piezoelectric transduction of the signal.
    • 本发明涉及一种分析免疫传感器装置及其构建方法,其基于适用于液体样品中任何靶抗原或分析物的检测和定量的聚合物和亲和元件的自组装单层的顺序沉积, 并允许实施“准无试剂”或“无试剂”位移测定,使非特异性吸附最小化,结果是检测限的降低。 所述装置是基于覆盖有聚合物单层(氧化还原或非氧化还原)的传感器表面而设计的,其上沉积有亲和元件的亚单层,以便随后进行与抗原,假抗原或抗原的亲和反应 标记有蛋白质,优选酶(氧化还原或非氧化还原)或纳米颗粒的半抗原),允许用于构建该装置以开发“点菜”免疫传感器的方法,其中信号的电化学,光学和/或压电转导 。