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1. 发明授权

EP2343362B8 Improved process for the culturing of cells 有权转让
标题翻译：用于细胞培养的改进的方法
公开(公告)号：EP2343362B8
公开(公告)日：2016-05-18
申请号：EP10183677.3
申请日：2007-07-04
申请人： DPx Holdings B.V.
发明人： Zijlstra, Gerben Meile , Hof, Robert Patrick , Schilder, Jacob
IPC分类号： C12M3/06 , C12M1/12 , C12N5/07 , C12N5/079 , C12P21/00
CPC分类号： C12P21/00 , B01D15/26 , B01D69/08 , C07K1/18 , C07K1/22 , C07K1/34 , C07K16/00 , C12M23/28 , C12M29/04 , C12M29/18 , C12M47/10 , C12N5/0603 , C12N5/0621 , C12N2500/32 , C12N2500/90 , C12N2500/92 , C12N2500/99 , C12N2510/04 , C12P21/005

2. 发明公开

EP2041259A1 IMPROVED PROCESS FOR THE CULTURING OF CELLS 有权转让
标题翻译：改进方法用于细胞培养
公开(公告)号：EP2041259A1
公开(公告)日：2009-04-01
申请号：EP07765049.7
申请日：2007-07-04
申请人： DSM IP Assets B.V.
发明人： ZIJLSTRA, Gerben, Meile , HOF, Robert, Patrick , SCHILDER, Jacob
IPC分类号： C12M1/12 , C12M3/06 , C12N5/06
CPC分类号： C12P21/00 , B01D15/26 , B01D69/08 , C07K1/18 , C07K1/22 , C07K1/34 , C07K16/00 , C12M23/28 , C12M29/04 , C12M29/18 , C12M47/10 , C12N5/0603 , C12N5/0621 , C12N2500/32 , C12N2500/90 , C12N2500/92 , C12N2500/99 , C12N2510/04 , C12P21/005
摘要： The invention relates to a process for the culturing of cells, preferably E1-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cell culture comprising the cells, the biological substance and cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture.

3. 发明授权

EP2634250B1 Improved process for the culturing of cells 有权转让
公开(公告)号：EP2634250B1
公开(公告)日：2017-04-05
申请号：EP13169694.0
申请日：2007-07-04
申请人： Patheon Holdings I B.V.
发明人： Zijlstra, Gerben Meile , Hof, Robert Patrick , Schilder, Jacob
IPC分类号： C12N5/071 , C12N5/079 , B01D15/26 , B01D69/08 , C07K16/00 , C07K1/18 , C07K1/22 , C07K1/34 , C12N5/073 , C12M1/12 , C12M3/06 , C12P21/00
CPC分类号： C12P21/00 , B01D15/26 , B01D69/08 , C07K1/18 , C07K1/22 , C07K1/34 , C07K16/00 , C12M23/28 , C12M29/04 , C12M29/18 , C12M47/10 , C12N5/0603 , C12N5/0621 , C12N2500/32 , C12N2500/90 , C12N2500/92 , C12N2500/99 , C12N2510/04 , C12P21/005
摘要： The invention relates to a process for the culturing of cells, preferably E1-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cell culture comprising the cells, the biological substance and cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture.

4. 发明公开

EP2593556A1 (R)-SELECTIVE AMINATION 有权转让
标题翻译：加利福尼亚州VERFAHREN ZUR（R）-SELEKTIVE AMINATION
公开(公告)号：EP2593556A1
公开(公告)日：2013-05-22
申请号：EP11733837.6
申请日：2011-07-14
申请人： DSM IP Assets B.V.
发明人： SCHÜRMANN, Martin , PEETERS, Wijnand Peter Helena , SMEETS, Natascha Hubertina Johannes , SCHWAB, Helmut , STEINER, Kerstin , LYPETSKA, Kateryna Mykolayivna , STROHMEIER, Gernot
IPC分类号： C12P13/00 , C12N9/10 , C12N1/14 , C12N1/20 , C12N15/54
CPC分类号： C12P13/001 , C12N9/1096 , C12Y206/01
摘要： The present invention relates to a method for the enzymatic synthesis of enantiomerically enriched (R)-amines of general formula [1][c], from the corresponding ketones of general formula [1][a], by using novel transaminases. These novel transaminases are selected from two different groups: either from a group of some 20 proteins with sequences as specified herein, or from a group of proteins having transaminase activity and isolated from a microorganism selected from the group of organisms consisting of Rahnella aquatilis, Ochrobactrum anthropi, Ochrobactrum tritici, Sinorhizobium morelense, Curtobacterium pusilllum, Paecilomyces lilacinus, Microbacterium ginsengisoli, Microbacterium trichothecenolyticum, Pseudomonas citronellolis, Yersinia kristensenii, Achromobacter spanius, Achromobacter insolitus, Mycobacterium fortuitum, Mycobacterium frederiksbergense, Mycobacterium sacrum, Mycobacterium fluoranthenivorans, Burkholderia sp., Burkholderia tropica, Cosmospora episphaeria, and Fusarium oxysporum.
摘要翻译：本发明涉及通过使用新型转氨酶从通式[1] [a]的相应酮酶促合成通式[1] [c]的对映体富集的（R） - 胺的方法。这些新型转氨酶选自两个不同的组：来自约20个具有本文所述序列的蛋白质的组，或来自具有转氨酶活性的一组蛋白质，并且从选自以下的微生物中分离：微生物，其由Rahnella aquatilis，Ochrobactrum anthropi，苍白杆菌小麦，苜蓿morelense，Curtobacterium属pusiffium，淡紫拟青霉，微ginsengisoli，微trichothecenolyticum，假单胞菌citronellolis，耶尔森kristensenii，无色spanius，无色insolitus，偶发分枝杆菌，结核分枝frederiksbergense，结核分枝骶骨，结核分枝fluoranthenivorans，Burkhoideria属，Burkhoideria tropica ，宇宙孢子囊菌和尖孢镰刀菌（Fusarium oxysporum）。

5. 发明公开

EP2222396A1 SYSTEM AND METHOD FOR PRODUCING BEADS 有权转让
标题翻译： VERFAHREN ZUR HERSTELLUNG VON PERLEN
公开(公告)号：EP2222396A1
公开(公告)日：2010-09-01
申请号：EP08858384.4
申请日：2008-12-02
申请人： DSM IP Assets B.V.
发明人： LIHME, Allan Otto Fog , HANSEN, Marie Bendix , HOLTE, René Oehlenschlaeger , LARSEN, Brian
IPC分类号： B01J13/04
CPC分类号： B01J2/08 , B01J13/046
摘要： The present invention relates to a method for producing beads comprising a material capable of gelation, said method comprising the steps of: (i) combining (a) a liquid composition comprising a material capable of gelation; and (b) a first hydrophobic phase; (ii) subjecting the liquid composition and the first hydrophobic phase, to means for emulsification in a first reactor by addition of external mechanical energy creating an emulsion comprising individual droplets comprising the material capable of gelation in the first hydrophobic phase (wherein the material capable of gelation provides a discontinuous phase and the first hydrophobic phase provides a continuous phase); (iii) stabilising the droplets by transferring the emulsion from the first reactor to a stabilisation reactor wherein the emulsion obtained in step (ii) is subjected to means for gelation in order to obtain gelation within 5 minutes or less, and the beads are formed.
摘要翻译：本发明涉及包含能够凝胶化的材料的珠粒的制造方法，所述方法包括以下步骤：（i）组合（a）包含能够凝胶化的材料的液体组合物; 和（b）第一疏水相; （ii）使液体组合物和第一疏水相经由添加外部机械能在第一反应器中进行乳化的装置，产生包含单个液滴的乳液，所述液滴包含能够在第一疏水相中凝胶化的材料（其中能够凝胶化提供不连续相，第一疏水相提供连续相）; （iii）通过将乳液从第一反应器转移到稳定反应器中来稳定液滴，其中在步骤（ii）中获得的乳液经受凝胶化的手段，以便在5分钟以内获得凝胶化，并形成珠粒。

6. 发明公开

EP2139573A1 EXPANDED BED COLUMN AND DISPOSABLE CHROMATOGRAPHY 有权转让
标题翻译：膨胀床柱，一次色谱
公开(公告)号：EP2139573A1
公开(公告)日：2010-01-06
申请号：EP08735566.5
申请日：2008-03-28
申请人： UPFRONT CHROMATOGRAPHY A/S
发明人： LIHME, Allan , HOLTE, René, Oehlenschläger , JENSEN, Kurt, Hauge , CHRISTENSEN, Tony
IPC分类号： B01D15/18 , G01N30/38
CPC分类号： B01D15/1807 , C07K1/22 , G01N30/38
摘要： In the field of expanded bed adsorption chromatography, with particular but not exclusive relevance to disposable expanded bed chromatography columns, a method of conducting upward flow expanded bed chromatography comprising: supplying a liquid via an inlet to a stationary phase medium contained in a column, allowing adsorption of at least one component from the liquid by the stationary phase medium, withdrawing the liquid from the column via an outlet, regulating the expansion of the stationary phase medium by regulation of the flow rate of the liquid through at least the inlet, and restricting any overpressure in the headspace of the column to not more than the outside pressure plus 0.1 bar, is provided. In addition, apparatus for use in said method, in particular columns for use in expanded bed chromatography, are provided.

7. 发明授权

EP2041259B2 IMPROVED PROCESS FOR THE CULTURING OF CELLS 有权
公开(公告)号：EP2041259B2
公开(公告)日：2024-10-09
申请号：EP07765049.7
申请日：2007-07-04
IPC分类号： C12M1/12 , C12M3/06 , C12N5/07 , C12N5/073 , C12N5/079
CPC分类号： C12N5/0603 , C12N5/0621 , C12N2500/3220130101 , C12N2510/0420130101 , C12N2500/9020130101 , C12N2500/9220130101 , C12M23/28 , C12M29/04 , C12M29/18 , C12M47/10 , B01D15/26 , C07K1/18

8. 发明授权

EP2634243B2 IMPROVED PROCESS FOR THE CULTURING OF CELLS 有权转让
公开(公告)号：EP2634243B2
公开(公告)日：2024-07-24
申请号：EP13169693.2
申请日：2007-07-04
IPC分类号： C12M1/12 , C12M3/06 , C12P21/00 , C12N5/071 , B01D15/26 , B01D69/08 , C07K16/00 , C07K1/18 , C07K1/22 , C07K1/34 , C12N5/073
CPC分类号： C12N5/0603 , C12N5/0621 , C12N2500/3220130101 , C12N2510/0420130101 , C12N2500/9020130101 , C12N2500/9220130101 , C12M23/28 , C12M29/04 , C12M29/18 , C12M47/10 , B01D15/26 , C07K1/18

9. 发明授权

EP2343362B2 Improved process for the culturing of cells 有权转让
公开(公告)号：EP2343362B2
公开(公告)日：2024-04-03
申请号：EP10183677.3
申请日：2007-07-04
发明人： Zijlstra, Gerben Meile , Hof, Robert Patrick , Schilder, Jacob
IPC分类号： C12M3/06 , C12M1/12 , C12N5/07 , C12N5/079 , C12P21/00

10. 发明授权

EP2593556B1 ENZYMATIC METHOD FOR (R)-SELECTIVE AMINATION 有权转让
标题翻译：（R） - 亲核胺化的酶法
公开(公告)号：EP2593556B1
公开(公告)日：2017-08-30
申请号：EP11733837.6
申请日：2011-07-14
申请人： DPx Holdings B.V.
发明人： SCHÜRMANN, Martin , PEETERS, Wijnand Peter Helena , SMEETS, Natascha Hubertina Johannes , SCHWAB, Helmut , STEINER, Kerstin , LYPETSKA, Kateryna Mykolayivna , STROHMEIER, Gernot
IPC分类号： C12P13/00 , C12N9/10 , C12N1/14 , C12N1/20 , C12N15/54
CPC分类号： C12P13/001 , C12N9/1096 , C12Y206/01
摘要： The present invention relates to a method for the enzymatic synthesis of enantiomerically enriched (R)-amines of general formula [1][c], from the corresponding ketones of general formula [1][a], by using novel transaminases. These novel transaminases are selected from two different groups: either from a group of some 20 proteins with sequences as specified herein, or from a group of proteins having transaminase activity and isolated from a microorganism selected from the group of organisms consisting of Rahnella aquatilis, Ochrobactrum anthropi, Ochrobactrum tritici, Sinorhizobium morelense, Curtobacterium pusilllum, Paecilomyces lilacinus, Microbacterium ginsengisoli, Microbacterium trichothecenolyticum, Pseudomonas citronellolis, Yersinia kristensenii, Achromobacter spanius, Achromobacter insolitus, Mycobacterium fortuitum, Mycobacterium frederiksbergense, Mycobacterium sacrum, Mycobacterium fluoranthenivorans, Burkholderia sp., Burkholderia tropica, Cosmospora episphaeria, and Fusarium oxysporum.
摘要翻译：本发明涉及通过使用新型转氨酶从相应的通式[1] [a]的酮酶合成通式[1] [c]的对映体富集的（R） - 胺的方法。这些新型转氨酶选自两个不同的组：从具有本文具体说明的序列的约20种蛋白质的组中选出，或选自具有转氨酶活性的蛋白质组中的一组蛋白质，并且从选自下列的微生物中分离：Rahnella aquatilis，Ochrobactrum anthropi，苍白杆菌小麦，苜蓿morelense，Curtobacterium属pusilllum，淡紫拟青霉，微ginsengisoli，微trichothecenolyticum，假单胞菌citronellolis，耶尔森kristensenii，无色spanius，无色insolitus，偶发分枝杆菌，结核分枝frederiksbergense，结核分枝骶骨，结核分枝fluoranthenivorans，鼻疽菌，类鼻疽tropica ，Cosmospora episphaeria和Fusarium oxysporum。

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