会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 6. 发明公开
    • NACHWEISVERFAHREN UNTER VERWENDUNG VON REKOMBINANTEN LEBENDEN ZELLEN ZUM NACHWEIS VON XENOBIOTISCHEN STOFFEN SOWIE ANORDNUNG UND TESTKIT ZUR DURCHFÜHRUNG DES NACHWEISVERFAHRENS
    • 鉴定方法重组活细胞检测物质的整理与检测试剂盒外源性实施的检测方法
    • EP3180615A1
    • 2017-06-21
    • EP15760363.0
    • 2015-07-10
    • Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung
    • BICKMEYER, Ulf-Georg
    • G01N33/53
    • G01N33/5014C12Q1/6897G01N21/6428G01N33/5008G01N33/5308G01N35/00871G01N2021/6439G01N2333/43504
    • Known detection methods for foreign substances mark gene sequences of living cells and detect, in a spatially resolved manner, the translocation of the stably expressed marked fusion constructions within the recombinant cells under the influence of foreign substance. The detection method according to the invention utilizes the dynamic expression behavior of recombinant living cells and detects in an integral manner. The cell-inherent defense system is preferably used, wherein gene sequences a
      i -m
      i in recombinant cells (19) as a preferred means in a test kit for performing the detection method code for transporter proteins A
      i comprising fluorescent marker proteins M
      i as fusion constructs A
      i -M
      i . The cell-inherent defense system is activated under the influence of foreign substance. Transporter proteins A
      i that transport the foreign substance out of the living cells (19) are expressed in an increased manner. Together with the transporter proteins A
      i , the marker proteins M
      i are also expressed in an increased manner, which is optically detected and enables corresponding conclusions about the foreign substance (03). The detection method is preferably implemented by means of an arrangement (04) of genetically modified aquatic organisms (06) or living cells (19) thereof, which are permanently exposed in a water-permeable habitat tank (05) in an aquatic system (01) in the vicinity of a technical installation (02).
    • 已知的用于异物检测的方法标记活细胞的基因序列和检测,在空间上分辨的方式,对稳定地过表达转位标记异物的影响下重组细胞内的融合结构。 雅丁到本发明的检测方法是利用重组的活细胞的动态行为的表达和在整体的方式检测英寸 小区固有防御系统,优选使用worin基因序列AI-MI在重组细胞(19),为优选的方法中的测试试剂盒用于转运蛋白艾执行检测方法代码包括荧光标记蛋白宓为融合构建艾 星期三 小区固有的防御系统被外来物质的作用下被激活。 转运蛋白艾做运输异物出来的活细胞(19)在增加的方式过表达的。 连同转运蛋白因此艾,标记蛋白祢在增加的方式过度表达,在所有被光学检测和启用关于异物(03)相应的结论。 该检测方法优选地通过一个装置(04)来实现遗传修饰的水生生物(06)或活细胞(19)它们的,这是在在水生系统中的水可渗透的栖息地罐(05)永久地暴露的(01 )在技术设备(02)附近。
    • 7. 发明公开
    • DIFFUSIONSKAMMER ZUR ERMITTLUNG UNTERSCHIEDLICHER PARAMETER EINER WÄSSRIGEN SUBSTANZ
    • DIFFUSIONSKAMMER ZUR ERMITTLUNG UNTERSCHIEDLICHER PARAMETER EINERWÄSSRIGENSUBSTANZ
    • EP3017290A2
    • 2016-05-11
    • EP14789977.7
    • 2014-06-21
    • Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung
    • OELLERMANN, MichaelMARK, Felix, ChristopherDUNKER, Erich
    • G01N21/01G01N21/03G01N33/49
    • G01N33/4925G01J3/42G01N21/01G01N21/0303G01N21/27G01N21/31G01N21/80G01N33/492G01N2021/035
    • Known diffusion chambers for measuring the pH value and absorption of aqueous substance samples take measurements with a relatively low resolution, large substance sample quantities, or in a non-simultaneous manner in the same sample. The diffusion chamber (01) according to the invention is equipped with a high-resolution pH optode (07) and a compact spectrophotometer (13), the respective glass fiber optics of which are integrated into the central cylinder (03) of the diffusion chamber (01) by means of special structural measures. In the process, the pH optode (07) is designed in the form of a syringe and is inserted into a sample slide (20), the cannula (11) being inserted into a diagonal groove (32) and guided through a sealing ring (22) on the sample slide (20) in a gas-tight manner. Thus, very small substance samples (05) (
    • 用于测量水溶性物质样品的pH值和吸收率的已知扩散室在相同样品中以相对低分辨率,大物质样品量或以非同时方式进行测量。 根据本发明的扩散室(01)配备有高分辨率pH光电极(07)和紧凑型分光光度计(13),其各自的玻璃纤维光学器件被集成到扩散室的中心圆柱体(03)中 (01)通过特殊的结构措施。 在该过程中,pH光极(07)被设计成注射器的形式并被插入到样品载玻片(20)中,套管(11)被插入对角槽(32)中并被引导通过密封环( 22)以气密方式固定在样品载玻片(20)上。 因此,可以测量非常小的物质样品(05)(<20μl),同时仍然确保测量尖端(28)浸入物质样品(05)的边缘区域(29)中而不中断光束路径(30 )进行吸收测量。 分光光度计的供光和取光光纤被集成到上,下圆柱形光学系统支架(23,25)中,其中样品室(04)通过插入的下光学系统支架(25) 隔圈(27),并确保准直透镜(26)和样品(05)之间的最小距离。 例如,当确定来自非常小的生物体的少量血液的氧结合曲线时,可以使用扩散室(01)。