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    • 2. 发明公开
      EP1049370A4 SESQUITERPENE SYNTHASES FROM GRAND FIR $i(ABIES GRANDIS), AND METHODS OF USE 审中-公开
    • SESQUITERPENE SYNTHASES FROM GRAND FIR $i(ABIES GRANDIS), AND METHODS OF USE
    • SESQUITERPENSYNTHASEN DER GROSSTANNE（ABIES GRANDIS）和方法ZU DEREN VERWENDUNG
    • EP1049370A4
    • 2004-10-06
    • EP99904185
    • 1999-01-21
    • UNIV WASHINGTON
    • CROTEAU RODNEY B , CROCK JOHN E , BOHLMAN JORG , JETTER REINHARD , STEELE CHRISTOPHER L
    • C12N9/88 , C12N15/82 , C12N15/84 , C12N15/60 , A01H5/00 , C12N5/10
    • C12N15/8243 , C12N9/88 , C12N15/8279
    • cDNAs encoding E-α-bisabolene synthase, δ-selinene synthase and η-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12; SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-α-bisabolene synthase (SEQ ID No:13), δ-selinene synthase (SEQ ID No:20) and η-humulene synthase (SEQ ID No:24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-α-bisabolene synthase, δ-selinene synthase and η-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-α-bisabolene synthase, δ-selinene synthase or η-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-α-bisabolene synthase, δ-selinene synthase or η-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant E-α-bisabolene synthase, δ-selinene synthase and η-humulene synthase may be used to obtain expression or enhanced expression of E-α-bisabolene synthase, δ-selinene synthase and η-humulene synthase in plants in order to enhance the production of sesquiterpenoids, or may be otherwise employed for the regulation or expression of E-α-bisabolene synthase, δ-selinene synthase and η-humulene synthase, or the production of their products.
    • 编码来自Grand Fir（Abies grandis）的E-α-赤柏烯合酶，δ-芹菜烯合酶和η-hum烯烯合成酶的cDNA已经被分离和测序，并且已经确定了相应的氨基酸序列。 因此，提供了分别编码E-α-赤柏烯合酶（SEQ ID No：13），δ-芹菜烯合酶（SEQ ID No：13），SEQ ID No： （Abies grandis）分别获得了一种分别来自禾本科（SEQ ID No：20）和η-hum月桂烯合酶（SEQ ID No：24）的核酸分子。 在其它方面，提供了编码E-α-赤柏烯合酶，δ-芹菜烯合酶和η-hum烯合酶或与至少一部分E-α-赤柏松烯合酶充分互补的碱基序列的可复制重组克隆载体， δ-海芹烯合酶或η-hum烯合成酶DNA或RNA以与其杂交。 在其他方面，提供了已经转化，转染，感染和/或注射了重组克隆载体和/或编码E-α-赤柏烯合酶，δ-芹菜烯合酶或η-hum lene烯合酶的DNA序列的修饰的宿主细胞。 因此，提供了用于上述重组倍半萜合酶的重组表达的系统和方法，所述重组倍半萜合酶可以用于促进其显着量的产生，分离和纯化。 重组E-α-赤藓烯合酶，δ-芹菜素合酶和η-hum烯合成酶可用于获得植物中E-α-赤柏烯合酶，δ-芹菜素合酶和η-hum烯合成酶的表达或增强的表达，以增强 倍半萜类化合物的生产，或者可以另外用于调节或表达E-α-赤柏烯合酶，δ-芹菜素合酶和η-hum烯合成酶或其产物的生产。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
    • 3. 发明公开
      EP1124943A4 NUCLEIC ACIDS ENCODING TAXUS GERANYLGERANYL DIPHOSPHATE SYNTHASE, AND METHODS OF USE 审中-公开
    • NUCLEIC ACIDS ENCODING TAXUS GERANYLGERANYL DIPHOSPHATE SYNTHASE, AND METHODS OF USE
    • NUKLEINSÄUREN，WELCHEFÜRTAXUS GERRANYLGERANYL DIPHOSPHATE SYNTHASE KODIERENUND VERFAHREN DER ANWENDUNG
    • EP1124943A4
    • 2002-04-10
    • EP99971845
    • 1999-10-27
    • UNIV WASHINGTON
    • CROTEAU RODNEY B , HEFNER JERRY L
    • C12N15/09 , C12N5/10 , C12N9/10 , C12N15/82 , C12R1/91 , C12N9/00 , C07H21/04 , C07K1/00 , C12N1/20 , C12N15/00
    • C12N9/1085 , C12N15/8243 , C12Y205/01029
    • A cDNA encoding geranylgeranyl diphosphate synthase from Canadian Yew (Taxus canadensis) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ NO:1) is provided which codes for the expression of geranylgeranyl diphosphate synthase (SEQ ID NO:2), from Canadian Yew (Taxus canadensis). In other aspects, replicable recombinant cloning vehicles are provided which code for geranylgeranyl diphosphate synthase, or for a base sequence sufficiently complementary to at least a portion of geranylgeranyl diphosphate synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranylgeranyl disphosphate synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant geranylgeranyl diphosphate synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant geranylgeranyl diphosphate synthase may be used to obtain expression or enhanced expression of geranylgeranyl diphosphate synthase in plants in order to enhance the production of geranylgeranyl diphosphate, or diterpenes derived therefrom, or may be otherwise employed for the regulation or expression of geranylgeranyl diphosphate synthase, or the production of its products.
    • 已经分离并测序了来自加拿大红豆杉（Taxus canadensis）的编码香叶基香叶基二磷酸合成酶的cDNA，并测定了相应的氨基酸序列。 因此，提供编码来自加拿大红豆杉（Taxus canadensis）的香叶基香叶基二磷酸合成酶（SEQ ID NO：2）的表达的分离的DNA序列（SEQ ID NO：1）。 在其它方面，提供编码香叶基香叶基二磷酸合成酶的可重复克隆载体，或与至少一部分香叶基香叶基二磷酸合酶DNA或RNA足够互补的碱基序列，以使其能够进行杂交。 在另一方面，提供已经用编码香叶基香叶基二磷酸合成酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。 因此，提供了用于重组表达上述重组er牛儿基ger牛儿基二磷酸合成酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。 可以使用重组香叶基香叶基二磷酸合成酶来获得表达或增强er牛儿基ger牛儿基二磷酸合成酶在植物中的表达，以增强er牛儿基ger牛儿基二磷酸酯的产生，或由其衍生的二萜，或可用于调节或表达香叶基香叶基二磷酸合成酶，或 其产品的生产。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
    • 4. 发明公开
      EP1228238A4 NUCLEIC ACID MOLECULES ENCODING ISOPENTENYL MONOPHOSPHATE KINASE, AND METHODS OF USE 审中-公开
    • NUCLEIC ACID MOLECULES ENCODING ISOPENTENYL MONOPHOSPHATE KINASE, AND METHODS OF USE
    • 异戊烯基MONO磷酸激酶CODING及其应用的核酸分子和方法
    • EP1228238A4
    • 2005-05-25
    • EP00975555
    • 2000-11-02
    • UNIV WASHINGTON
    • CROTEAU RODNEY B , LANGE BERND M
    • C12N9/12 , C12P21/06 , C07H21/04 , C12N1/20 , C12N9/00 , C12N15/00
    • C12N9/12
    • A cDNA encoding isopentenyl monophosphate kinase (IPK) from peppermint ( Mentha x piperita ) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of isopentenyl monophosphate kinase (SEQ ID NO:2), from peppermint ( Mentha x piperita ). In other aspects, replicable recombinant cloning vehicles are provided which code for isopentenyl monophosphate kinase, or for a base sequence sufficiently complementary to at least a portion of isopentenyl monophosphate kinase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding isopentenyl monophosphate kinase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant isopentenyl monophosphate kinase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant isopentenyl monophosphate kinase may be used to obtain expression or enhanced expression of isopentenyl monophosphate kinase in plants in order to enhance the production of isopentenyl monophosphate kinase, or isoprenoids derived therefrom, or may be otherwise employed for the regulation or expression of isopentenyl monophosphate kinase, or the production of its products.
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
    • 7. 发明公开
      EP1021459A4 ISOLATION AND EXPRESSION OF FARNESENE SYNTHASE FROM PEPPERMINT, $i(MENTHA X PIPERITA, L.) 审中-公开
    • ISOLATION AND EXPRESSION OF FARNESENE SYNTHASE FROM PEPPERMINT, $i(MENTHA X PIPERITA, L.)
    • 分离和FARNESYLSYNTHASE OFF薄荷的表达（薄荷X piperita L.）
    • EP1021459A4
    • 2004-08-11
    • EP98953251
    • 1998-10-05
    • UNIV WASHINGTON
    • CROTEAU RODNEY B , WILDUNG MARK R , CROCK JOHN E
    • C12N9/88 , C12P5/02 , C12N15/60 , C12N5/10
    • C12P5/007 , C12N9/88
    • A cDNA encoding (E)-β-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-β-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-β-farnesene synthase, or for base sequence sufficiently complementary to at least a portion of (E)-β-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-β-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-β-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-β-farnesene synthase may be used to obtain expression or enhanced expression of (E)-β-farnesene synthase in plants in order to enhance the production of (E)-β-farnesene, or may be otherwise employed for the regulation or expression of (E)-β-farnesene synthase, or the production of its product.
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
    • 9. 发明公开
      EP1032257A4 MONOTERPENE SYNTHASES FROM GRAND FIR ($i(ABIES GRANDIS)) 失效
    • MONOTERPENE SYNTHASES FROM GRAND FIR ($i(ABIES GRANDIS))
    • FIR的单萜合酶（冷杉桉）
    • EP1032257A4
    • 2005-03-16
    • EP98935641
    • 1998-07-10
    • UNIV WASHINGTON
    • BOHLMANN JOERG , STEELE CHRISTOPHER L , CROTEAU RODNEY B
    • A23D9/00 , C11B1/00 , C12N9/88 , C12N15/82 , A01H5/00 , A01H5/10 , A23D7/00 , A23K1/14 , C07H21/04 , C07K4/10 , C12N15/04 , C12N15/63
    • A23D9/00 , C11B1/00 , C12N9/88 , C12N15/8242 , C12N15/8243
    • cDNAs encoding myrcene synthase, (-)-limonene synthase and (-)-pinene synthase from Grand fir (Abies grandis) haven been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID NO:1; SEQ ID NO:3 and SEQ ID NO:5) are provided which code for the expression of myrcene synthase (SEQ ID NO:2), (-)-pinene synthase (SEQ ID NO:4) and (-)-limonene synthase (SEQ ID NO:6) , respectively, from Grand fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for myrcene synthase, (-)-limonene synthase and (-)-pinene synthase, or for a base sequence sufficiently complementary to at least a portion of myrcene synthase, (-)-limonene synthase or (-)-pinene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding myrcene synthase, (-)-limonene synthase or (-)-pinene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant myrcene synthase, (-)-limonene synthase or (-)-pinene synthase may be used to obtain expression or enhanced expression of myrcene synthase, (-)-limonene synthase or (-)-pinene synthase in plants in order to enhance the production of monoterpenoids, or may be otherwise employed for the regulation or expression of myrcene synthase, (-)-limonene synthase and (-)-pinene synthase, or the production of their products.
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
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