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1. 发明公开

EP2732038A1 METHODS OF TRANSCRIPTION ACTIVATOR LIKE EFFECTOR ASSEMBLY 有权
标题翻译： VERFAHREN ZUR TRANSKRIPTION EINERAKTIVATORÄHNLICHENEFFEKTORANORDNUNG
公开(公告)号：EP2732038A1
公开(公告)日：2014-05-21
申请号：EP12814750.1
申请日：2012-07-12
申请人： The General Hospital Corporation
发明人： JOUNG, J., Keith , SANDER, Jeffry, D.
IPC分类号： C12N15/66 , C12N15/62 , C12N15/31 , C12Q1/68 , C07K19/00 , C07K14/24 , C12N1/00 , C12N5/00 , C40B40/06
CPC分类号： C07K14/195 , C07K2319/00 , C07K2319/24 , C07K2319/41 , C07K2319/43 , C07K2319/80 , C12N9/22 , C12N15/1093 , C12N15/66 , C12P19/34 , C12P21/02 , C12Q1/6806 , C12Y301/00 , C40B40/08 , C40B50/06 , C12Q2521/501 , C12Q2563/149
摘要： The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and second sets.
摘要翻译：本公开描述了包括提供具有编码包含一个或多个转录激活子样效应子（TALE）重复结构域和/或一个或多个TALE重复结构域的一个或多个部分的第一组的序列的第一核酸的方法; 使第一核酸与第一酶接触，其中第一酶产生第一可结合末端; 提供具有编码包含一个或多个TALE重复结构域和/或一个或多个TALE重复结构域的一个或多个部分的第二组的序列的第二核酸; 使所述第二核酸与第二酶接触，其中所述第二酶产生第二可连接末端，并且其中所述第一和第二可连接末端是相容的; 以及通过所述第一和第二可连接末端连接所述第一和第二核酸以产生第一连接的核酸，其中所述第一连接的核酸与固体支持物连接，并且其中所述第一连接的核酸编码包含所述第一和第二核酸的多肽第二套

2. 发明公开

EP3461896A3 METHODS OF TRANSCRIPTION ACTIVATOR LIKE EFFECTOR ASSEMBLY 有权
公开(公告)号：EP3461896A3
公开(公告)日：2019-06-26
申请号：EP18191841.8
申请日：2012-07-12
申请人： The General Hospital Corporation
发明人： JOUNG, J., keith , SANDER, Jeffry, D.
IPC分类号： C12N15/66 , C12N15/62 , C12N15/31 , C12Q1/68 , C07K19/00 , C07K14/24 , C12N1/00 , C12N5/00 , C40B40/06
摘要： The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and second sets.

3. 发明公开

EP3461896A2 METHODS OF TRANSCRIPTION ACTIVATOR LIKE EFFECTOR ASSEMBLY 有权
公开(公告)号：EP3461896A2
公开(公告)日：2019-04-03
申请号：EP18191841.8
申请日：2012-07-12
申请人： The General Hospital Corporation
发明人： JOUNG, J., keith , SANDER, Jeffry, D.
IPC分类号： C12N15/66 , C12N15/62 , C12N15/31 , C12Q1/68 , C07K19/00 , C07K14/24 , C12N1/00 , C12N5/00 , C40B40/06
CPC分类号： C07K14/195 , C07K2319/00 , C07K2319/24 , C07K2319/41 , C07K2319/43 , C07K2319/80 , C12N9/22 , C12N15/1093 , C12N15/66 , C12P19/34 , C12P21/02 , C12Q1/6806 , C12Y301/00 , C40B40/08 , C40B50/06 , C12Q2521/501 , C12Q2563/149
摘要： The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and second sets.

4. 发明授权

EP2732038B1 METHODS OF TRANSCRIPTION ACTIVATOR LIKE EFFECTOR ASSEMBLY 有权
公开(公告)号：EP2732038B1
公开(公告)日：2018-09-05
申请号：EP12814750.1
申请日：2012-07-12
申请人： The General Hospital Corporation
发明人： JOUNG, J., Keith , SANDER, Jeffry, D.
IPC分类号： C12N15/66 , C12N15/62 , C12N15/31 , C12Q1/68 , C07K19/00 , C07K14/24 , C12N1/00 , C12N5/00 , C40B40/06
CPC分类号： C07K14/195 , C07K2319/00 , C07K2319/24 , C07K2319/41 , C07K2319/43 , C07K2319/80 , C12N9/22 , C12N15/1093 , C12N15/66 , C12P19/34 , C12P21/02 , C12Q1/6806 , C12Y301/00 , C40B40/08 , C40B50/06 , C12Q2521/501 , C12Q2563/149
摘要： The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and second sets.

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