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1. 发明公开

EP0246912A2 Method and compositions for protecting anerobic microorganisms 失效
标题翻译： Verfahren und Zusammensetzungenfürden Schutz von anaeroben Mikroorganismen。
公开(公告)号：EP0246912A2
公开(公告)日：1987-11-25
申请号：EP87304571.0
申请日：1987-05-22
申请人： SYNTEX (U.S.A.) INC.
发明人： Armenta, Richard D. , Ullman, Edwin F. , Gibbons, Ian
IPC分类号： C12M1/04 , C12N1/00 , C12N1/04 , C12N9/04
CPC分类号： C12N1/04 , C12M23/08 , C12M23/32 , C12M23/34 , C12M23/38 , C12M41/34 , Y10S435/801 , Y10S435/818
摘要： A method is disclosed for reducing the oxygen content of a medium in which are present cells, usually anaerobic microorganisms, to extend the time during which the cells remain viable. The method comprises having in fluid contact with the aqueous medium an effective amount of an oxidase and substrate for the oxidase. The oxidase and substrate for the oxidase in an aqueous medium can be in fluid, e.g., air, contact with a separately contained aqueous medium in which the cells are present. Alternatively, the cells can be present in the same aqueous medium as the oxidase and substrate for the oxidase. The aqueous medium containing the oxidase and the substrate for the oxidase can further contain a hydrogen peroxide scavenger in an effective amount. A composition in accordance with the invention comprises in a liquid medium viable cells, such as anaerobic microorganisms, an oxidase and its substrate in an amount sufficient to retain the viability of the cells, and a hydrogen peroxide scavenger in an amount sufficient to reduce the level of hydrogen peroxide generated by the oxidase. The method of the invention has particular application in transport media used for transporting microbiological specimens.
摘要翻译：公开了一种减少其中存在细胞（通常是厌氧微生物）延长细胞保持存活时间的培养基的氧含量的方法。该方法包括与水性介质流体接触有效量的用于氧化酶的氧化酶和底物。用于水介质中氧化酶的氧化酶和底物可以是流体，例如空气，与存在细胞的单独含有的水性介质接触。或者，细胞可以存在于与氧化酶和氧化酶底物相同的水性介质中。含有氧化酶的水性介质和氧化酶底物可进一步含有有效量的过氧化氢清除剂。根据本发明的组合物在液体介质中包含足以保持细胞存活力的量的活细胞，例如厌氧微生物，氧化酶及其底物，以及足以降低水平的量的过氧化氢清除剂的氧化酶产生的过氧化氢。本发明的方法特别适用于运输微生物标本的运输介质。

2. 发明公开

EP0140602A2 Immunofluorescence detection composition and method 有权转让
标题翻译： Immunofluoreszierender证明组合物和方法。
公开(公告)号：EP0140602A2
公开(公告)日：1985-05-08
申请号：EP84306802.4
申请日：1984-10-05
申请人： SYNTEX (U.S.A.) INC.
发明人： Khanna, Pyarre , Gibbons, Ian , Allen, Jimmy D.
IPC分类号： G01N33/53
CPC分类号： G01N33/542 , Y10S435/975 , Y10S436/80 , Y10S436/825
摘要： Novel compositions and methods are provided for detecting the presence of a material of interest in a specimen on a solid surface. A composition useful for detecting the presence of a material of interest in a specimen comprises (1) a detector conjugate comprising a fluorescing moiety bonded to a compound capable of specific binding with the material of interest, or a derivative thereof, and (2) a non-detector conjugate comprising a poly(amino acid) and a compound having substantial structural and charge similarity to the fluorescing compound and no observable fluorescence, or low level fluorescence of a different wavelength than that of the fluorescing compound, in the region of fluorescence of the fluorescing compound. In the method of the invention, a specimen, on a solid surface, is combined with the detector conjugate and the non-detector conjugate, and the combination is incubated. Unbound conjugates are removed from the solid surface by washing and then the surface is irradiated with light having a wave length absorbed by the fluorescing compound. The fluorescent light emitted by the combination is observed and is a function of the amount of the material of interest present in the specimen.

3. 发明授权

EP0243001B1 Liquid single reagent for assays 有权
标题翻译：单液体试剂进行测试。
公开(公告)号：EP0243001B1
公开(公告)日：1993-10-20
申请号：EP87302511.8
申请日：1987-03-24
申请人： SYNTEX (U.S.A.) INC.
发明人： Gibbons, Ian , Felgner, Philip L. , Ullman, Edwin F.
IPC分类号： G01N33/546 , G01N33/549 , G01N33/555 , A61K9/50 , A61K47/00
CPC分类号： G01N33/549 , G01N33/54313

4. 发明公开

EP0243001A1 Liquid single reagent for assays 有权
标题翻译： Einzelnesflüssiges试剂。
公开(公告)号：EP0243001A1
公开(公告)日：1987-10-28
申请号：EP87302511.8
申请日：1987-03-24
申请人： SYNTEX (U.S.A.) INC.
发明人： Gibbons, Ian , Felgner, Philip L. , Ullman, Edwin F.
IPC分类号： G01N33/546 , G01N33/549 , G01N33/555 , A61K9/50 , A61K47/00
CPC分类号： G01N33/549 , G01N33/54313
摘要： Assay methods and compositions are provided for determining an analyte in a sample suspected of containing the analyte. The composition comprises in a novel single liquid reagent at least one specific binding pair (sbp) member and its complementary member wherein at least one sbp member is reversibly confined in a material that temporarily renders the confined sbp member incapable of binding with its complementary sbp member. At least one of the sbp members is bound to a member of a signal producing system capable of producing a detectable signal in relation to the amount of analyte in the sample. The confinement is reversed, any remaining members of the signal producing system are added, and the signal produced in relation to the amount of analyte is measured.
摘要翻译：提供测定方法和组合物用于测定怀疑含有分析物的样品中的分析物。组合物包含新颖的单液体试剂至少一种特异性结合对（sbp）构件及其互补构件，其中至少一个sbp构件可逆地限制在暂时使受限制的sbp构件不能与其互补的sbp构件结合的材料中。 sbp成员中的至少一个与能够产生与样品中分析物的量相关的可检测信号的信号产生系统的成员结合。约束相反，添加信号产生系统的任何剩余成员，并且测量相对于分析物量产生的信号。

5. 发明公开

EP0246912A3 Method and compositions for protecting anerobic microorganisms 失效
标题翻译：用于保护无机微生物的方法和组合物
公开(公告)号：EP0246912A3
公开(公告)日：1988-11-02
申请号：EP87304571
申请日：1987-05-22
申请人： SYNTEX (U.S.A.) INC.
发明人： Armenta, Richard D. , Ullman, Edwin F. , Gibbons, Ian
IPC分类号： C12M01/04 , C12N01/00 , C12N01/04 , C12N09/04
CPC分类号： C12N1/04 , C12M23/08 , C12M23/32 , C12M23/34 , C12M23/38 , C12M41/34 , Y10S435/801 , Y10S435/818
摘要： A method is disclosed for reducing the oxygen content of a medium in which are present cells, usually anaerobic microorganisms, to extend the time during which the cells remain viable. The method comprises having in fluid contact with the aqueous medium an effective amount of an oxidase and substrate for the oxidase. The oxidase and substrate for the oxidase in an aqueous medium can be in fluid, e.g., air, contact with a separately contained aqueous medium in which the cells are present. Alternatively, the cells can be present in the same aqueous medium as the oxidase and substrate for the oxidase. The aqueous medium containing the oxidase and the substrate for the oxidase can further contain a hydrogen peroxide scavenger in an effective amount. A composition in accordance with the invention comprises in a liquid medium viable cells, such as anaerobic microorganisms, an oxidase and its substrate in an amount sufficient to retain the viability of the cells, and a hydrogen peroxide scavenger in an amount sufficient to reduce the level of hydrogen peroxide generated by the oxidase. The method of the invention has particular application in transport media used for transporting microbiological specimens.

6. 发明授权

EP0140602B1 Immunofluorescence detection composition and method 有权转让
标题翻译：免疫荧光检测组合物和方法
公开(公告)号：EP0140602B1
公开(公告)日：1991-01-16
申请号：EP84306802.4
申请日：1984-10-05
申请人： SYNTEX (U.S.A.) INC.
发明人： Khanna, Pyarre , Gibbons, Ian , Allen, Jimmy D.
IPC分类号： G01N33/53
CPC分类号： G01N33/542 , Y10S435/975 , Y10S436/80 , Y10S436/825

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