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1. 发明授权

EP1507002B1 Thermostable Taq polymerase fragment 有权
标题翻译：热稳定Taq DNA聚合酶片段
公开(公告)号：EP1507002B1
公开(公告)日：2006-12-20
申请号：EP04018913.6
申请日：2004-08-10
申请人： Roche Diagnostics GmbH , F. HOFFMANN-LA ROCHE AG
发明人： Ankenbauer, Waltraud, Dr. , Meier, Thomas, Dr. , Deufel, Annette, Dr. , Heindl, Dieter, Dr. , Betzl, Gisela, Dr. , Schmuck, Rainer, Dr. , Schneidinger, Bernd, Dr. , Strey, Jessica
IPC分类号： C12N9/12
CPC分类号： C12N9/1252

2. 发明公开

EP0921196A1 Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction 失效
标题翻译：从Carboxydothermus生氢聚合酶修饰的DNA以及其用于耦合逆转录和聚合酶链式反应中使用
公开(公告)号：EP0921196A1
公开(公告)日：1999-06-09
申请号：EP97121151.1
申请日：1997-12-02
申请人： Roche Diagnostics GmbH
发明人： Ankenbauer, Waltraud, Dr. , Markau, Ursula , Ebenbichler, Christine , Achhammer, Gunthar
IPC分类号： C12N15/31 , C12N9/12 , C12Q1/68
CPC分类号： C12N9/1252
摘要： A purified DNA polymerase exhibiting reverse transcriptase activity in the presence of magnesium ions and/or manganese ions having reduced or no 5'-3'-exonuclease activity and substantially no RNaseH activity and obtainable from Carboxydothermus hydrogenoformans .
摘要翻译：纯化的DNA聚合酶在镁离子和/或锰离子存在参展逆转录酶活性具有降低的或从Carboxydothermus生氢没有5'-3“外切酶活性和基本上没有RNA酶H活性和可获得的。

3. 发明公开

EP0922765A1 Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction 有权
标题翻译：从Corboxydothermus生氢修饰的DNA聚合酶及其用于耦合逆转录和聚合酶链式反应中使用的
公开(公告)号：EP0922765A1
公开(公告)日：1999-06-16
申请号：EP98122533.7
申请日：1998-11-30
申请人： Roche Diagnostics GmbH
发明人： Ankenbauer, Waltraud, Dr. , Markau, Ursula , Ebenbichler, Christine , Achhammer, Gunthar, Dr.
IPC分类号： C12N15/31 , C12N9/12 , C12Q1/68
CPC分类号： C12N9/1252
摘要： A purified DNA polymerase exhibiting reverse transcriptase activity in the presence of magnesium ions and/or manganese ions having reduced or no 5'-3'-exonuclease activity and substantially no RNaseH activity and obtainable from Carboxydothermus hydrogenoformans.
摘要翻译：纯化的DNA聚合酶在镁离子和/或锰离子存在参展逆转录酶活性具有降低的或从Carboxydothermus生氢没有5'-3“外切酶活性和基本上没有RNA酶H活性和可获得的。

4. 发明公开

EP1507002A3 Thermostable Taq polymerase fragment 有权
标题翻译：热稳定剂Taq聚合酶片段
公开(公告)号：EP1507002A3
公开(公告)日：2005-07-27
申请号：EP04018913.6
申请日：2004-08-10
申请人： Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： Ankenbauer, Waltraud, Dr. , Meier, Thomas, Dr. , Deufel, Annette, Dr. , Heindl, Dieter, Dr. , Betzl, Gisela, Dr. , Schmuck, Rainer, Dr. , Schneidinger, Bernd, Dr. , Strey, Jessica
IPC分类号： C12N9/12
CPC分类号： C12N9/1252
摘要： It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (TaqΔ288) possesses an increased thermostability over TaqWT, TaqΔ279, and TaqΔ289. The present invention therefore provides TaqΔ288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for TaqΔ288. The invention also encompasses kits containing TaqΔ288 as well as the use of TaqΔ288 and kits containing TaqΔ288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.

5. 发明公开

EP1277841A2 New composition and method for hot start nucleic acid amplification 有权
标题翻译： Neue Zusammensetzung und Methode zur'hot start'Nukleinsäureamplifizierung
公开(公告)号：EP1277841A2
公开(公告)日：2003-01-22
申请号：EP02015234.4
申请日：2002-07-09
申请人： Roche Diagnostics GmbH , F. HOFFMANN-LA ROCHE AG
发明人： Ankenbauer, Waltraud, Dr. , Heindl, Dieter, Dr. , Laue, Frank , Huber, Andreas
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2521/319 , C12Q2525/186 , C12Q2549/101
摘要： The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3'-5' Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3' terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.
摘要翻译：本发明涉及一种用于进行核酸扩增反应的新组合物，其包含（i）热稳定的DNA聚合酶，（ii）热稳定的3'-5'核酸外切酶，和（iii）用于核酸扩增的至少一个引物具有未被所述耐热性DNA聚合酶延长的修饰的3'末端残基以及使用该组合物进行PCR反应的方法。此外，该方法涉及包含这种组合物的试剂盒。

6. 发明授权

EP1502961B1 New detection format for hot start real time polymerase chain reaction 有权
标题翻译：用于检测Heissstart-和实时聚合酶链反应的新格式
公开(公告)号：EP1502961B1
公开(公告)日：2010-09-08
申请号：EP04017701.6
申请日：2004-07-27
申请人： Roche Diagnostics GmbH , F.HOFFMANN-LA ROCHE AG
发明人： Heindl, Dieter, Dr. , Ankenbauer, Waltraud, Dr. , Laue, Frank
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68

7. 发明授权

EP1277841B1 New composition and method for hot start nucleic acid amplification 有权
标题翻译：新组合物和的“热启动”的方法的核酸扩增
公开(公告)号：EP1277841B1
公开(公告)日：2009-08-26
申请号：EP02015234.4
申请日：2002-07-09
申请人： Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： Ankenbauer, Waltraud, Dr. , Heindl, Dieter, Dr. , Laue, Frank , Huber, Andreas
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2521/319 , C12Q2525/186 , C12Q2549/101

8. 发明授权

EP0922765B1 Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction 有权
标题翻译：从Corboxydothermus生氢修饰的DNA聚合酶及其用于耦合逆转录和聚合酶链式反应中使用的
公开(公告)号：EP0922765B1
公开(公告)日：2005-11-23
申请号：EP98122533.7
申请日：1998-11-30
申请人： Roche Diagnostics GmbH
发明人： Ankenbauer, Waltraud, Dr. , Markau, Ursula , Ebenbichler, Christine , Achhammer, Gunthar, Dr.
IPC分类号： C12N15/31 , C12N9/12 , C12Q1/68
CPC分类号： C12N9/1252

9. 发明公开

EP1507002A2 Thermostable Taq polymerase fragment 有权
标题翻译：热稳定剂Taq聚合酶片段
公开(公告)号：EP1507002A2
公开(公告)日：2005-02-16
申请号：EP04018913.6
申请日：2004-08-10
申请人： Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： Ankenbauer, Waltraud, Dr. , Meier, Thomas, Dr. , Deufel, Annette, Dr. , Heindl, Dieter, Dr. , Betzl, Gisela, Dr. , Schmuck, Rainer, Dr. , Schneidinger, Bernd, Dr. , Strey, Jessica
IPC分类号： C12N9/12
CPC分类号： C12N9/1252
摘要： It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (TaqΔ288) possesses an increased thermostability over TaqWT, TaqΔ279, and TaqΔ289. The present invention therefore provides TaqΔ288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for TaqΔ288. The invention also encompasses kits containing TaqΔ288 as well as the use of TaqΔ288 and kits containing TaqΔ288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.
摘要翻译：发现缺乏288个N-末端氨基酸（Taq DELTA 288）的天然的水生栖热菌水生DNA聚合酶（TaqWT）的片段比TaqWT，Taq DELTA 279和Taq DELTA 289具有增加的热稳定性。因此，本发明提供Taq DELTA 288，编码其相同或衍生物的重组表达载体以及Taq DELTA288的纯化方案。本发明还包括含有Taq DELTA 288的试剂盒，以及使用Taq DELTA 288和含有Taq DELTA288的试剂盒。此外，本发明包括用于测序核酸模板的方法和用于扩增靶核酸的方法。

10. 发明公开

EP1502961A1 New detection format for hot start real time polymerase chain reaction 有权
标题翻译： Neues格式zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion
公开(公告)号：EP1502961A1
公开(公告)日：2005-02-02
申请号：EP04017701.6
申请日：2004-07-27
申请人： Roche Diagnostics GmbH , F.HOFFMANN-LA ROCHE AG
发明人： Heindl, Dieter, Dr. , Ankenbauer, Waltraud, Dr. , Laue, Frank
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68
摘要： The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising a) subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of (i) a thermostable DNA Polymerase, (ii) a thermostable double strand dependent 3'-5' exonuclease having a temperature optimum above 37°C , (iii) a pair of amplification primers, (iv) Deoxynucleoside-Tri-Phosphates, (v) a detecting oligonucleotide carrying a first label and a second label, said first label being capable of acting as a fluorescent reporter entity when excited with light of an appropriate wavelength, said second label being capable of acting as a fluorescence quenching entity of said fluorescent reporter entity, characterized in that one label is bound to the 3' end of said detecting oligonucleotide, and further characterized in that the other label is bound either internally or at the 5' end to said detecting oligonucleotide, and b) monitoring fluorescence of said fluorerescent reporter entity at least after a plurality of amplification cycles.
摘要翻译：本发明涉及一种用于扩增和检测靶核酸的方法和组合物，其包括：a）在（i）耐热性DNA聚合酶，（ii）热稳定性聚合酶存在下使所述靶核酸进行实时PCR扩增反应温度最高在37℃以上的双链相关3'-5'外切核酸酶，（iii）一对扩增引物，（iv）脱氧核苷 - 三磷酸酯，（v）携带第一标记和第二标记的检测寡核苷酸所述第一标记当用适当波长的光激发时能够用作荧光报道实体，所述第二标记能够用作所述荧光报道实体的荧光猝灭实体，其特征在于一个标记结合到3 所述检测寡核苷酸的末端，其特征还在于另一个标记在内部或在5'端与所述检测寡核苷酸结合，以及b）监测荧光至少在多个扩增循环后，所述荧光报道实体的阳离子。

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