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1. 发明公开

EP2153219A2 METHOD AND SYSTEM FOR DETECTING PEPTIDE PEAKS IN HPLC-MS SIGNALS 审中-公开
标题翻译：用于检测HPLC-MS信号中的肽峰的方法和系统
公开(公告)号：EP2153219A2
公开(公告)日：2010-02-17
申请号：EP08776692.9
申请日：2008-05-30
申请人： Politecnico di Milano
发明人： LEVANDER, Fredrik , CAPPADONA, Salvatore , PATTINI, Linda , CERUTTI, Sergio , JAMES, Peter
IPC分类号： G01N30/86 , G06F17/00 , G06F17/14
CPC分类号： G01N30/8675 , G01N30/8603 , G01N30/8617 , G01N30/8641
摘要： The present invention relates to a method, a computer tool and a system for detecting peptide peaks in measurement signals (11) generated by HPLC-MS instruments. The method comprises the steps of: providing an intensity column vector (SIC) representative of the elution values at a specific mass value (m/z) of the measurement signal (11); performing a wavelet decomposition (15) of such values of said intensity column vector (SIC) to generate a first processed vector (16) representative of the smoothed intensity values of the intensity column vector (SIC); providing a threshold value (S) and performing a wavelet decomposition (17) as a function of the value of said threshold (S) to generate the second processed vector (18) representative of said intensity column vector (SIC) cleaned of any oscillations generated by stochastic noise (e(t)); processing said first processed vector (16) and said second processed vector (18) to generate a third processed vector (19) identifying the baseline values of said intensity column vector (SIC); processing said intensity column vector (SIC) and said second (18) and third (19) vectors to generate e filtered vector (20) identifying any peak value (ZJ) below the background noise (12).
摘要翻译：本发明涉及用于检测由HPLC-MS仪器产生的测量信号（11）中的肽峰的方法，计算机工具和系统。该方法包括以下步骤：提供表示在测量信号（11）的特定质量值（m / z）处的洗脱值的强度列矢量（SIC）; 对所述强度列向量（SIC）的这些值执行小波分解（15）以产生表示强度列向量（SIC）的平滑强度值的第一经处理向量（16）; 提供阈值（S）并根据所述阈值（S）的值执行小波分解（17）以产生表示所述强度列矢量（SIC）的第二经处理矢量（18），所述强度列矢量（SIC）通过随机噪声（e（t））; 处理所述第一处理矢量（16）和所述第二处理矢量（18）以产生识别所述强度列矢量（SIC）的基线值的第三处理矢量（19）; 处理所述强度列向量（SIC）和所述第二（18）和第三（19）向量以产生识别低于背景噪声（12）的任何峰值（ZJ）的e滤波向量（20）。

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