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1. 发明公开

EP2477026A1 IMAGE ANALYZING METHOD AND IMAGE ANALYZING DEVICE 审中-公开
标题翻译： BILDANALYSEVERFAHREN UND BILDANALYSEVORRICHTUNG
公开(公告)号：EP2477026A1
公开(公告)日：2012-07-18
申请号：EP10823479.0
申请日：2010-10-15
申请人： Olympus Corporation
发明人： SUZUKI, Akemi
IPC分类号： G01N21/64 , G06T7/20
CPC分类号： G02B21/0076 , G01J3/10 , G01J3/36 , G01J3/4406 , G01N21/6458 , G02B21/0032 , G02B21/008 , G02B21/0088
摘要： S1: An image of an observation region is acquired. S2: An analysis region is set on the image of the observation region. S3: A sub-analysis region smaller than the analysis region is set. S4 - S7: The sub-analysis region is intermittently moved in increments of a pixel interval over the entire analysis region, and correlation analysis is executed using data of pixels in the sub-analysis region every time the sub-analysis region is moved, so as to estimate at least a molecular number or diffusion constant of the sub-analysis region. S8: A molecular number or diffusion constant image is formed by mapping the molecular numbers or diffusion constants.
摘要翻译： S1：获取观察区域的图像。 S2：在观察区域的图像上设置分析区域。 S3：设定小于分析区域的子分析区域。 S4-S7：子分析区域在整个分析区域上以像素间隔的间隔间歇地移动，并且每次子分析区域移动时，使用子分析区域中的像素的数据执行相关分析，因此以估计亚分析区域的至少一个分子数或扩散常数。 S8：通过映射分子数或扩散常数形成分子数或扩散常数图像。

2. 发明公开

EP2096429A1 FLUORESCENT SIGNAL ANALYZING APPARATUS AND FLUORESCENT SIGNAL ANALYZING METHOD 审中-公开
标题翻译： VORRICHTUNG ZURANALYZE FLUORESZIERENDER SIGNALE UND VERFAHREN ZUR ANALYZE FLUORESZIERENDER SIGNALE
公开(公告)号：EP2096429A1
公开(公告)日：2009-09-02
申请号：EP08702920.3
申请日：2008-01-07
申请人： Olympus Corporation
发明人： SUZUKI, Akemi
IPC分类号： G01N21/64
CPC分类号： G01N21/6408 , G01N21/6456
摘要： A analyzing apparatus 160 includes an image input unit 162 to which at least one fluorescent image of a sample S is input, a storage unit 164 for storing data on the at least one fluorescent image input to the image input unit 162, a data extracting unit 172 for extracting, for each of the fluorescent images, data on a fluorescence intensity in a desired region on the at least one fluorescent image from the data on the at least one fluorescent image stored in the storage unit 164, and an analyzing unit 174 for calculating a correlation and a photon counting histogram for the desired region by use of the data on the fluorescence intensity extracted by the data extracting unit 172.
摘要翻译：分析装置160包括输入样本S的至少一个荧光图像的图像输入单元162，用于存储输入到图像输入单元162的至少一个荧光图像上的数据的存储单元164，数据提取单元 172，用于针对每个荧光图像从存储在存储单元164中的至少一个荧光图像上的数据中提取关于至少一个荧光图像上的期望区域中的荧光强度的数据;以及分析单元174，用于通过使用由数据提取单元172提取的荧光强度的数据来计算所需区域的相关性和光子计数直方图。

3. 发明授权

EP2623957B1 IMAGE ANALYSIS METHOD AND IMAGE ANALYSIS DEVICE 有权
公开(公告)号：EP2623957B1
公开(公告)日：2018-12-26
申请号：EP10858967.2
申请日：2010-10-29
申请人： Olympus Corporation
发明人： SUZUKI, Akemi
IPC分类号： G01N21/64 , G06T7/00
CPC分类号： G06K9/6202 , G01J3/4406 , G01N21/6408 , G01N21/6458 , G01N2021/6419 , G01N2021/6421 , G02B21/0076 , G02B21/008 , G06K9/0014 , G06T7/0012 , G06T2207/10056 , G06T2207/10064 , G06T2207/30024
摘要： Step S1: acquiring fluorescent images of frames in time-series. Each fluorescent image comprises pixels in which pixel data are acquired in the time-series. Step S2: setting analysis areas to the fluorescent images. Step S3: selecting the fluorescent images of two or more frames to be used in analysis. Step S4: extracting data pairs each comprising two pixels in which acquisition time intervals are the same in the analysis area of each of the fluorescent images selected in step S3, and performing product sum calculation of each of the data pairs for all of the selected images to calculate a correlation value.

4. 发明公开

EP2778659A1 IMAGE ANALYSIS METHOD AND IMAGE ANALYSIS DEVICE 无效转让
标题翻译： BILDANALYSEVERFAHREN UND BILDANALYSEVORRICHTUNG
公开(公告)号：EP2778659A1
公开(公告)日：2014-09-17
申请号：EP12848351.8
申请日：2012-10-04
申请人： Olympus Corporation
发明人： SUZUKI, Akemi
IPC分类号： G01N21/64
CPC分类号： G06K9/00147 , G01N21/6408 , G01N21/6458 , G02B21/0076 , G02B21/18 , G06K9/0014 , G06T7/97
摘要： S1: A fluorescence image of one or more frames of the observation region or the sample S is acquired. S2: One or more analytic regions are set for the fluorescence image of the observation region of one frame or each of frames that has been acquired. S3: Data on the pixel corresponding to each analytic region is extracted. S4: Time intervals of data pairs for use in correlation calculations are set. S6: A fitting is performed for the result of the correlation calculation. S7: The analytic result is saved. S8: The operations in Step S5 to Step S7 are repeated for each of the time intervals set in Step S4.
摘要翻译： S1：获取观察区域或样本S的一个或多个帧的荧光图像。 S2：对于已经获取的一帧或每帧的观察区域的荧光图像设置一个或多个分析区域。 S3：提取与各分析区域对应的像素上的数据。 S4：设置用于相关计算的数据对的时间间隔。 S6：进行相关计算的结果。 S7：保存分析结果。 S8：对于步骤S4中设定的每个时间间隔，重复步骤S5至步骤S7中的操作。

5. 发明公开

EP2623957A1 IMAGE ANALYSIS METHOD AND IMAGE ANALYSIS DEVICE 有权
标题翻译： BILDANALYSEVERFAHREN UND BILDANALYSEVORRICHTUNG
公开(公告)号：EP2623957A1
公开(公告)日：2013-08-07
申请号：EP10858967.2
申请日：2010-10-29
申请人： Olympus Corporation
发明人： SUZUKI, Akemi
IPC分类号： G01N21/64 , G06T7/00
CPC分类号： G06K9/6202 , G01J3/4406 , G01N21/6408 , G01N21/6458 , G01N2021/6419 , G01N2021/6421 , G02B21/0076 , G02B21/008 , G06K9/0014 , G06T7/0012 , G06T2207/10056 , G06T2207/10064 , G06T2207/30024
摘要： Step S1: acquiring fluorescent images of frames in time-series. Each fluorescent image comprises pixels in which pixel data are acquired in the time-series. Step S2: setting analysis areas to the fluorescent images. Step S3: selecting the fluorescent images of two or more frames to be used in analysis. Step S4: extracting data pairs each comprising two pixels in which acquisition time intervals are the same in the analysis area of each of the fluorescent images selected in step S3, and performing product sum calculation of each of the data pairs for all of the selected images to calculate a correlation value.
摘要翻译：步骤S1：以时间序列获取帧的荧光图像。每个荧光图像包括其中以时间序列获取像素数据的像素。步骤S2：将分析区域设置为荧光图像。步骤S3：选择要用于分析的两个或多个帧的荧光图像。步骤S4：在步骤S3中选择的每个荧光图像的分析区域中提取每个包括两个像素的数据对，其中采集时间间隔相同，并且对于所有选择的图像执行每个数据对的乘积和计算以计算相关值。

6. 发明公开

EP2623956A1 IMAGE ANALYSIS METHOD AND IMAGE ANALYSIS DEVICE 审中-公开
标题翻译：图像分析方法和图像分析装置
公开(公告)号：EP2623956A1
公开(公告)日：2013-08-07
申请号：EP10858965.6
申请日：2010-10-29
申请人： Olympus Corporation
发明人： SUZUKI, Akemi
IPC分类号： G01N21/64 , G06T7/00
CPC分类号： G06K9/628 , G01N21/6408 , G01N21/6458 , G06K9/0014 , G06T5/50 , G06T7/0012 , G06T2207/10056 , G06T2207/10064 , G06T2207/30024
摘要： Step S1: acquiring fluorescent images of frames in time-series. Each fluorescent image comprises pixels in which pixel data are acquired in the time-series. Step S2: setting analysis areas to the acquired fluorescent images. Step S3: calculating a classification value of the analysis areas R2. Step S4: classifying the images into one or more groups on the basis of the classification value. Step S5: calculating an average image of the analysis area every group. Step S6: subtracting the average image from each image of the analysis area every group to calculate a new image of the analysis area. Step S7: calculating a correlation value on the basis of the new images every group. Step S8: performing estimation of a diffusion time or diffusion constant, a molecule number, etc. by use of a fitting equation.
摘要翻译：步骤S1：按时间序列获取帧的荧光图像。每个荧光图像包括以时间序列获取像素数据的像素。步骤S2：将分析区域设置为获取的荧光图像。步骤S3：计算分析区域R2的分类值。步骤S4：根据分类值将图像分类为一个或多个组。步骤S5：计算每组分析区域的平均图像。步骤S6：从每个分析区域的每个图像中减去平均图像以计算分析区域的新图像。步骤S7：基于每组新图像计算相关值。步骤S8：通过使用拟合方程来执行扩散时间或扩散常数，分子数量等的估计。

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