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    • 3. 发明公开
    • CELL LOCATION UNIT, ARRAY, DEVICE AND FORMATION METHOD THEREOF
    • 小区位置单元,阵列,装置及其形成方法
    • EP3159400A1
    • 2017-04-26
    • EP15810868.8
    • 2015-06-23
    • Ocular Fluidics, Inc.
    • HUANG, ChengjunLUO, JunZHAO, Chao
    • C12M1/42B03C5/00
    • B03C5/005B03C5/00B03C5/022C12M1/42G01N27/44791G01N33/4836G01N33/48728
    • The present disclosure provides a cell positioning unit, array, device and a method for manufacturing the same. The cell positioning unit comprises: a substrate; at least a pair of microelectrodes on the substrate, wherein the microelectrodes are disposed at intervals on a circumference such that pDEP force are generated in an area where tips of the microelectrodes aggregate, and wherein a voltage signal applied to at least one microelectrode has a phase difference with a voltage signal applied to another microelectrode; and a cell positioning hole on the microelectrodes, wherein the cell positioning hole has an accommodation space which exposes the pDEP force fields and accommodates a single cell. By applying an AC voltage signal having a certain amplitude, frequency and phase to the microelectrodes, cells will move to surfaces of the microelectrodes, such that a single cell can be accurately positioned to a certain place by means of the cell positioning hole and the microelectrodes.
    • 本公开提供了一种单元定位单元,阵列,器件及其制造方法。 该单元定位单元包括:衬底; 在所述基板上的至少一对微电极,其中所述微电极在圆周上间隔设置,使得在所述微电极的尖端聚集的区域中产生pDEP力,并且其中施加到至少一个微电极的电压信号具有相位 与施加到另一微电极的电压信号的差异; 以及微电极上的细胞定位孔,其中细胞定位孔具有暴露pDEP力场并容纳单细胞的容纳空间。 通过对微电极施加具有一定幅度,频率和相位的交流电压信号,细胞将移动到微电极的表面,使得单细胞可以通过细胞定位孔和微电极精确定位到特定位置 。