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1. 发明授权

EP3000823B1 STRONG SECRETORY SIGNAL PEPTIDE ENHANCING SMALL PEPTIDE MOTIF AND USE THEREOF 有权
公开(公告)号：EP3000823B1
公开(公告)日：2019-06-12
申请号：EP14791458.4
申请日：2014-04-25
申请人： Nanjing University of Technology
发明人： HE, Bingfang , CHEN, Wenhua , MI, Lan , WU, Shanshan , ZHU, Yun , CHEN, Shanshan
IPC分类号： C07K5/103 , C07K7/06 , C07K7/08 , C07K14/00 , C12N15/70 , C12N1/21 , C12N9/24 , C12N9/42 , C12N15/11

2. 发明公开

EP3000823A1 STRONG SECRETORY SIGNAL PEPTIDE ENHANCING SMALL PEPTIDE MOTIF AND USE THEREOF 审中-公开
标题翻译：小肽基序和使用强分泌信号肽固结THEREOF
公开(公告)号：EP3000823A1
公开(公告)日：2016-03-30
申请号：EP14791458.4
申请日：2014-04-25
申请人： Nanjing University of Technology
发明人： HE, Bingfang , CHEN, Wenhua , MI, Lan , WU, Shanshan , ZHU, Yun , CHEN, Shanshan
IPC分类号： C07K5/103 , C07K7/06 , C07K7/08 , C07K14/00 , C12N15/70 , C12N1/21 , C12N9/24 , C12N9/42 , C12N15/11
CPC分类号： C07K7/06 , C07K7/08 , C07K14/00 , C07K2319/02 , C12N9/2402 , C12N9/2454 , C12N15/625 , C12P21/02 , C12Y302/01 , C12Y302/01011
摘要： The present invention belongs to protein engineering and genetic engineering fields, relating to a strong secretory signal peptide enhancing small peptide motifs and the use thereof. The strong secretory signal peptide enhancing small peptide motifs of the present invention have the amino acid sequence of the following formula: M (αXβYγ/αYβXγ) n , wherein X represents an acidic amino acid; Y represents an alkaline amino acid; α is 0 to 2 neutral amino acid(s); β represents 0 to 2 neutral amino acid(s); γ represents 1 to 10 neutral amino acid(s); n is 1 to 3. With regard to the use of the strong secretory signal peptide enhancing small peptide motifs of the present invention, it is a method for constructing a vector enhancing the secretion ability of common signal peptides to improve the secretory expression of exogenous proteins.

3. 发明授权

EP2615100B1 FRUCTOSYLATED PUERARIN, AND PREPARATION METHOD THEREFOR AND USE THEREOF 有权
标题翻译： Fructosylated葛根，工艺用于生产和使用
公开(公告)号：EP2615100B1
公开(公告)日：2015-07-15
申请号：EP11831919.3
申请日：2011-09-23
申请人： Nanjing University of Technology
发明人： HE, Bingfang , WU, Xueming , CHU, Jianlin , WU, Bin , ZHANG, Sen , OUYANG, Pingkai
IPC分类号： C07H17/07 , A61K31/7048 , C12P19/60 , A61P9/00 , A61P35/00
CPC分类号： C07H15/26 , A61K31/353 , A61K31/7048 , C12N1/20 , C12P17/162 , C12P19/58 , C12P19/605

4. 发明授权

EP3301106B1 STRONG SECRETORY SIGNAL PEPTIDE ENHANCING SMALL PEPTIDE MOTIF AND USE THEREOF 有权
公开(公告)号：EP3301106B1
公开(公告)日：2019-06-12
申请号：EP17200981.3
申请日：2014-04-25
申请人： Nanjing University of Technology
发明人： HE, Bingfang , CHEN, Wenhua , MI, Lan , WU, Shanshan , ZHU, Yun , CHEN, Shanshan
IPC分类号： C07K5/103 , C07K7/06 , C07K7/08 , C07K14/00 , C12N15/70 , C12N1/21 , C12N9/24 , C12N9/42 , C12N15/11

5. 发明公开

EP3000823A9 STRONG SECRETORY SIGNAL PEPTIDE ENHANCING SMALL PEPTIDE MOTIF AND USE THEREOF 审中-公开
公开(公告)号：EP3000823A9
公开(公告)日：2018-11-14
申请号：EP14791458.4
申请日：2014-04-25
申请人： Nanjing University of Technology
发明人： HE, Bingfang , CHEN, Wenhua , MI, Lan , WU, Shanshan , ZHU, Yun , CHEN, Shanshan
IPC分类号： C07K5/103 , C07K7/06 , C07K7/08 , C07K14/00 , C12N15/70 , C12N1/21 , C12N9/24 , C12N9/42 , C12N15/11
CPC分类号： C07K7/06 , C07K7/08 , C07K14/00 , C07K2319/02 , C12N9/2402 , C12N9/2454 , C12N15/625 , C12P21/02 , C12Y302/01 , C12Y302/01011
摘要： The present invention belongs to protein engineering and genetic engineering fields, relating to a strong secretory signal peptide enhancing small peptide motifs and the use thereof. The strong secretory signal peptide enhancing small peptide motifs of the present invention have the amino acid sequence of the following formula: M (αXβYγ/αYβXγ) n , wherein X represents an acidic amino acid; Y represents an alkaline amino acid; α is 0 to 2 neutral amino acid(s); β represents 0 to 2 neutral amino acid(s); γ represents 1 to 10 neutral amino acid(s); n is 1 to 3. With regard to the use of the strong secretory signal peptide enhancing small peptide motifs of the present invention, it is a method for constructing a vector enhancing the secretion ability of common signal peptides to improve the secretory expression of exogenous proteins.

6. 发明公开

EP3301106A1 STRONG SECRETORY SIGNAL PEPTIDE ENHANCING SMALL PEPTIDE MOTIF AND USE THEREOF 审中-公开
标题翻译：强分泌信号肽增强小肽片段及其应用
公开(公告)号：EP3301106A1
公开(公告)日：2018-04-04
申请号：EP17200981.3
申请日：2014-04-25
申请人： Nanjing University of Technology
发明人： HE, Bingfang , CHEN, Wenhua , MI, Lan , WU, Shanshan , ZHU, Yun , CHEN, Shanshan
IPC分类号： C07K5/103 , C07K7/06 , C07K7/08 , C07K14/00 , C12N15/70 , C12N1/21 , C12N9/24 , C12N9/42 , C12N15/11
CPC分类号： C07K7/06 , C07K7/08 , C07K14/00 , C07K2319/02 , C12N9/2402 , C12N9/2454 , C12N15/625 , C12P21/02 , C12Y302/01 , C12Y302/01011
摘要： The present invention belongs to protein engineering and genetic engineering fields, relating to a strong secretory signal peptide enhancing small peptide motifs and the use thereof. The strong secretory signal peptide enhancing small peptide motifs of the present invention have the amino acid sequence of the following formula: M (αXβYγ/αYβXγ) n , wherein X represents an acidic amino acid; Y represents an alkaline amino acid; α is 0 to 2 neutral amino acid(s); β represents 0 to 2 neutral amino acid(s); γ represents 1 to 10 neutral amino acid(s); n is 1 to 3. With regard to the use of the strong secretory signal peptide enhancing small peptide motifs of the present invention, it is a method for constructing a vector enhancing the secretion ability of common signal peptides to improve the secretory expression of exogenous proteins.
摘要翻译：本发明属于蛋白质工程和基因工程领域，涉及强分泌信号肽增强小肽基序及其用途。本发明的强分泌信号肽增强小肽基序具有下式的氨基酸序列：M（αXβYγ/αYβXγ）n，其中X代表酸性氨基酸; Y代表碱性氨基酸; α是0至2个中性氨基酸; β代表0至2个中性氨基酸; γ代表1至10个中性氨基酸; n是1至3.关于本发明的强分泌信号肽增强小肽基序的用途，它是构建增强常见信号肽分泌能力的载体的方法，以改善外源蛋白质的分泌表达。

7. 发明授权

EP2657245B1 FRUCTOSYLATED MANGIFERIN AND PREPARATION METHOD THEREFOR AND USE THEREOF 有权
标题翻译： Fructosylated芒果，工艺用于生产和使用
公开(公告)号：EP2657245B1
公开(公告)日：2014-06-18
申请号：EP12807633.8
申请日：2012-03-08
申请人： Nanjing University of Technology
发明人： HE, Bingfang , WU, Xueming , CHU, Jianlin , WU, Bin , ZHANG, Sen , OUYANG, Pingkai
IPC分类号： C07H15/26 , A61K31/7048 , A61P35/00 , A61P35/02 , C12P19/58
CPC分类号： C07H15/26 , C12P17/06 , C12P19/00 , C12P19/14

8. 发明公开

EP2657245A1 FRUCTOSYLATED MANGIFERIN AND PREPARATION METHOD THEREFOR AND USE THEREOF 有权
标题翻译： FRUCTOSYLATED MANGIFERIN及其制备方法及其用途
公开(公告)号：EP2657245A1
公开(公告)日：2013-10-30
申请号：EP12807633.8
申请日：2012-03-08
申请人： Nanjing University of Technology
发明人： HE, Bingfang , WU, Xueming , CHU, Jianlin , WU, Bin , ZHANG, Sen , OUYANG, Pingkai
IPC分类号： C07H15/26 , A61K31/7048 , A61P35/00 , A61P35/02 , C12P19/58
CPC分类号： C07H15/26 , C12P17/06 , C12P19/00 , C12P19/14
摘要： Provided are a fructosylated mangiferin, a preparation method therefor and a use thereof, wherein the fructosylated mangiferin has a structural formula represented by the following formula (I), the method for preparing the fructosylated mangiferin includes adding a substance with fructosylating enzymatic activity to a transformed liquid containing mangiferin for biotransformation reaction, so as to convert the mangiferin into the fructosylated mangiferin, wherein the transformed liquid contains the mangiferin and a glycosyl donor; as well as a use of the fructosylated mangiferin in preparation of a medicament for treatment of tumor-related diseases.
摘要翻译：本发明提供一种果糖基化芒果苷及其制备方法及其用途，其中所述果糖基化芒果苷具有下式（I）所示的结构式，所述果糖基化芒果苷的制备方法包括将具有果糖基化酶活性的物质加入转化体将含有芒果苷的液体进行生物转化反应，以将芒果苷转化为果糖基化芒果苷，其中转化液体含有芒果苷和糖基供体; 以及果糖基化芒果苷在制备用于治疗肿瘤相关疾病的药物中的用途。

9. 发明公开

EP2615100A1 FRUCTOSYLATED PUERARIN, AND PREPARATION METHOD THEREFOR AND USE THEREOF 有权
标题翻译：贝壳葡萄酒（VERWENDUNG，FRERGOYLIERTES PUERARIN，HERSTELLUNGSVERFAHRENDAFÜRUND SEINE VERWENDUNG）
公开(公告)号：EP2615100A1
公开(公告)日：2013-07-17
申请号：EP11831919.3
申请日：2011-09-23
申请人： Nanjing University of Technology
发明人： HE, Bingfang , WU, Xueming , CHU, Jianlin , WU, Bin , ZHANG, Sen , OUYANG, Pingkai
IPC分类号： C07H17/07 , C12P19/60 , A61K31/7048 , A61P9/00 , A61P35/00
CPC分类号： C07H15/26 , A61K31/353 , A61K31/7048 , C12N1/20 , C12P17/162 , C12P19/58 , C12P19/605
摘要： This invention is in the field of bio-pharmaceutical technology, specifically involving the new derivatives of puerarin, the biological conversion methods and their pharmaceutical applications. This invention published a new type fructosylated puerarin with structure as shown in Formula (I) below, its preparation method being converting puerarin into fructosyl and fructose oligosaccharyl puerarin in a aqueous phase or nonaqueous phase system by bioconversion method, and at a puerarin concentration of 107.5g/L, the monofructosyl-β (2,6)-puerarin concentration can reach 111.7g/L, and conversion rate of fructosylated puerarin is 90%. By adsorption with AB-8 macroporous resin and gradient elution, product with purity over 97% and complying drug specification can be obtained, with extraction rate over 80%. Tests have shown that fructosylated puerarin is effective to acute myocardio ischemia, and can markedly suppress in vitro the proliferation of human breast cancer cell strain MDA-MB-23 and human chronmyelogenors leukemia cell strain K562, and such oligosaccharylated puerarin has low toxicity, with good application prospects in making drugs to treat cardio-cerebral vascular diseases and/or tumour diseases.
摘要翻译：本发明在生物制药技术领域，具体涉及葛根素的新衍生物，生物转化方法及其制药应用。本发明公开了一种具有如下式（I）所示结构的新型果糖基葛根素，其制备方法是通过生物转化法将葛根素转化为水相或非水相体系中的果糖基和果糖寡糖基葛根素，葛根素浓度为107.5 g / L，单糖基-2（2,6） - 葛根素浓度可达111.7g / L，果糖基葛根素的转化率为90％。通过AB-8大孔树脂吸附和梯度洗脱，可以得到纯度超过97％，符合药品规格的产品，提取率超过80％。测试结果表明，果糖醛酸葛根素对急性心肌缺血有效，体外可显着抑制人乳腺癌细胞株MDA-MB-23和人类慢性白血病细胞株K562的增殖，而这种寡糖基化的葛根素毒性低，具有良好的制造药物治疗心脑血管疾病和/或肿瘤疾病的应用前景。

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