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    • 3. 发明公开
    • PCR PRIMER SET FOR BACTERIAL DNA AMPLIFICATION, KIT FOR DETECTING AND/OR IDENTIFYING BACTERIAL SPECIES, AND METHOD FOR DETECTING AND/OR IDENTIFYING BACTERIAL SPECIES
    • PCR引物对细菌DNA扩增,KIT用于检测和/或鉴定的细菌种类和方法用于检测和/或用于鉴定物种
    • EP3056567A1
    • 2016-08-17
    • EP14853073.6
    • 2014-10-07
    • Mitsui Chemicals, Inc.
    • AMANO, KohSAKAI, TomomiYANAI, HisaakiTABATA, HomareMINAMI, HiroshiKITAJIMA, IsaoNIIMI, Hideki
    • C12N15/09C12M1/00C12M1/34C12Q1/04C12Q1/68
    • C12Q1/689C12Q2600/16
    • An object of the present invention is to provide a primer set useful for further improvement of sensitivity in detection or identification of bacterial species in a sample by a PCR method, a kit for PCR using the primer set and a method of detection or identification of bacterial species in a sample using the primer set, as well as to further improve sensitivity in detection or identification of bacterial species in a sample by conducting a PCR method using the primer set with a minimized contamination amount of bacterial nucleic acid. A primer set according to the present invention comprises at least one primer pair in seven primer pairs obtained by selecting one primer pair from each of Groups S1 to S7 consisting of specific primers in order to achieve the above objects. In addition, a kit for detection of bacterial species according to the present invention comprises at least one primer pair in seven primer pairs obtained by selecting one primer pair from each of Groups K1 to K7 consisting of specific primers.
    • 本发明的一个目的是提供一种引物通过PCR方法,使用该引物组及检测的方法或细菌鉴定PCR的试剂盒,设置在检测或样品中的细菌物种的鉴定灵敏度的进一步改进有用 一个样品中的物种使用引物组,以及つweiterer通过进行使用引物与污染细菌核酸的最小量来设定一个PCR方法改善的样品中检测或细菌物种的识别灵敏度。 雅丁设定为,本发明的引物包括在通过选择从每个组S1中的一个引物对为S7,以实现上述目的的特异性引物由......组成得到7个引物对的至少一个引物对。 另外,用于检测细菌物种gemäß的对本发明的试剂盒包括在通过选择从每个组K1中的一个引物对K7由...组成特异性引物获得7的引物对的至少一个引物对。