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    • 2. 发明公开
    • EXTRACELLULAR POTENTIAL MEASURING DEVICE AND ITS MANUFACTURING METHOD
    • VERFAHREN ZU IHRER HERSTELLUNG的超细纤维
    • EP1602922A1
    • 2005-12-07
    • EP04718412.2
    • 2004-03-08
    • MATSUSHITA ELECTRIC INDUSTRIAL CO., LTD.
    • NAKATANI, MasayaOKA, HiroakiEMOTO, Fumiaki
    • G01N27/30G01N27/416C12Q1/02C12M1/34G01N33/483
    • G01N33/48728
    • An extracellular potential measuring device includes a plate portion having a first surface and a second surface opposite to the first surface, and an electrode provided on the second surface of the plate portion. In the plate portion, a pocket having an opening which opens to the first surface is formed, and a through-hole communicating to the second surface from the pocket. The through-hole communicates from a position which is closer to the opening than a deepest point of the first pocket. The electrode is provided around of the opening of the through-hole. In this device, even if a cell to be examined does not reach the deepest point of the pocket, a cell membrane of the cell can tightly attaches onto the through-hole securely without a clearance. Hence, culture solution inside the through-hole is isolated from culture solution over an upper surface of the plate portion, thereby allowing electrochemical changes caused by activities of the cell to be detected efficiently with a detector electrode.
    • 细胞外电位测量装置包括具有第一表面和与第一表面相对的第二表面的板部分,以及设置在板部分的第二表面上的电极。 在板部分中,形成有具有向第一表面开口的开口的口袋,以及从口袋连通到第二表面的通孔。 通孔从比第一口袋的最深点更靠近开口的位置连通。 电极设置在通孔的开口周围。 在该装置中,即使要检查的细胞未达到口袋的最深点,细胞的细胞膜可以牢固地紧贴在通孔上而没有间隙。 因此,通孔内部的培养液在培养液的上表面与培养液分离,由此可以用检测器电极有效地检测细胞的活性引起的电化学变化。