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1. 发明公开

EP1209234A1 STEREOSTRUCTURE OF DECARBAMYLASE AND METHOD OF USING THE SAME 审中-公开
标题翻译： VERFAHREN ZU DEREN VERWENDUNG的STEREOSTRUKTUR VON DECARBAMYLASE
公开(公告)号：EP1209234A1
公开(公告)日：2002-05-29
申请号：EP00956828.8
申请日：2000-08-30
申请人： Kaneka Corporation
发明人： NAKAI, Takahisa , MORIKAWA, Souichi , ISHII, Kiyoto , NANBA, Hirokazu , YAJIMA, Kazuyoshi , IKENAKA, Yasuhiro , TAKAHASHI, Satomi
IPC分类号： C12N15/60 , C12N9/88 , G06F17/30
CPC分类号： C12N9/80
摘要： The present invention reveals the stereostructure of decarbamylase by X-ray crystallography and provides a decarbamylase mutant, which is more useful for industrial applications, obtained by molecular design utilizing the stereostructure for the purpose of improving the reactivity of decarbamylase to D-N-carbamoyl-α-amino acid which is a substrate thereof. Specifically, the present invention relates to the stereostructure of decarbamylase determined by X-ray crystallography; a stereostructure model of a decarbamylase mutant; a stereostructure model of a complex thereof with a substrate, a product, and the like; a molecular design method utilizing these stereostructures; a decarbamylase mutant obtained by the method; and a method for designing and producing a protein mutant having a structure similar to that of decarbamylase.
摘要翻译：本发明通过X射线晶体学显示了脱氨基甲酰酶的立体结构，并且提供了一种更适用于工业应用的脱氨基甲酰酶突变体，其通过利用立体结构的分子设计获得，目的是改进脱氨基甲酰酶对DN-氨基甲酰基-α- 氨基酸是其底物。具体地说，本发明涉及通过X射线晶体学测定的脱氨基甲酰酶的立体结构; 脱氨基甲酰酶突变体的立体结构模型; 其与基材，产品等的复合物的立体结构模型; 利用这些立体结构的分子设计方法; 通过该方法获得的脱氨基甲酰酶突变体; 以及具有与脱氨基甲酰酶类似的结构的蛋白质突变体的设计和制备方法。

2. 发明公开

EP2465938A1 PRODUCTION METHOD FOR ASTAXANTHIN-CONTAINING COMPOSITION 审中-公开
标题翻译： VERFAHREN ZUR HERSTELLUNG EINER ASTAXANTHINHALTIGEN ZUSAMMENSETZUNG
公开(公告)号：EP2465938A1
公开(公告)日：2012-06-20
申请号：EP10808081.3
申请日：2010-08-11
申请人： Kaneka Corporation
发明人： INOUE, Hiroaki , KIZAKI, Noriyuki , NANBA, Hirokazu
IPC分类号： C12P23/00 , A23K1/16 , A23L1/30 , A61P3/00 , A61K31/01 , A61K31/122 , A61K35/74 , C12R1/01 , C12R1/645
CPC分类号： A61K31/01 , A23K20/174 , A23K20/179 , A61K31/122 , C12P23/00
摘要： The present invention relates to a method for reducing the relative ratio of 3-hydroxy-3',4'-didehydro-β,ψ-caroten-4-one (HDCO) to astaxanthin in a composition containing astaxanthin and HDCO by contacting the composition with an acidic medium having a pH of 3 or less and/or a basic medium having a pH of 9 or greater, and also relates to a method for producing an astaxanthin-containing composition which includes reducing the relative ratio of HDCO by the above method. By means of the method of the present invention, the relative ratio of HDCO, the biological function of which is not known, in an astaxanthin-containing composition can be easily reduced.
摘要翻译：本发明涉及通过使组合物与组合物接触来降低含有虾青素和HDCO的组合物中3-羟基-3'，4'-二脱氢-2，β-胡萝卜素-4-酮（HDCO）与虾青素的相对比例的方法 pH为3以下的酸性介质和/或pH为9以上的碱性介质，还涉及含有虾青素的组合物的制造方法，其包括通过上述方法降低HDCO的相对比例。通过本发明的方法，可以容易地降低含有虾青素的组合物中HDCO（其生物功能不知道）的相对比例。

3. 发明授权

EP1918375B1 D-AMINO ACID OXIDASE, AND METHOD FOR PRODUCTION OF L-AMINO ACID, 2-OXO ACID OR CYCLIC IMINE 有权转让
标题翻译： D-氨基酸氧化酶及其制造方法的L-氨基酸，2-氧代或环状ILOW
公开(公告)号：EP1918375B1
公开(公告)日：2015-03-11
申请号：EP06782154.6
申请日：2006-08-02
申请人： Kaneka Corporation
发明人： KANAMARU, Hiroyuki , UEDA, Makoto , MIKI, Ryuuji , NANBA, Hirokazu
IPC分类号： C12N15/09 , C12N1/15 , C12N1/21 , C12N9/06 , C12P7/40 , C12P13/04 , C12P17/10 , C12R1/19 , C12R1/72
CPC分类号： C12P7/40 , C12N9/0024 , C12P13/04 , C12P17/10

4. 发明公开

EP2072622A1 METHOD FOR PRODUCTION OF L-AMINO ACID 审中-公开
标题翻译： VERFAHREN ZUR HERSTELLUNG VON L-AMINOSÄURE
公开(公告)号：EP2072622A1
公开(公告)日：2009-06-24
申请号：EP07829543.3
申请日：2007-10-11
申请人： Kaneka Corporation
发明人： KANAMARU, Hiroyuki , UEDA, Makoto , NANBA, Hirokazu
IPC分类号： C12P13/04 , C12N15/09
CPC分类号： C12P13/06 , C12N9/0008 , C12N9/0073 , C12P13/04 , C12P13/08 , C12P13/222
摘要： The present invention has its object to provide a method for producing an L-amino acid comprising reacting a keto acid with an amino acid dehydrogenase and an enzyme having coenzyme regenerating ability to convert to a L-amino acid, wherein a coenzyme is added in two or more portions in the reaction. The method of the present invention enables efficient production of an L-amino acid useful as a synthetic intermediate such as a pharmaceutical intermediate with high optical purity by an enzymatic reductive amination independent of the purity of the keto acid used as a substrate.
摘要翻译：本发明的目的是提供一种生产L-氨基酸的方法，其包括使酮酸与氨基酸脱氢酶反应的方法和具有辅酶再生能力的酶转化为L-氨基酸，其中将辅酶加入二或更多的部分。本发明的方法能够独立于用作底物的酮酸的纯度，通过酶还原胺化作用，有效地生产可用作合成中间体的L-氨基酸，例如具有高光学纯度的药物中间体。

5. 发明公开

EP1852510A1 5-SUBSTITUTED HYDANTOIN RACEMASE, DNA ENCODING THE SAME, RECOMBINANT DNA, TRANSFORMED CELL, AND PROCESS FOR PRODUCTION OF OPTICALLY ACTIVE N-CARBAMYLAMINO ACID OR OPTICALLY ACTIVE AMINO ACID 审中-公开
标题翻译： 5-取代的乙内酰脲消旋酶编码DNA，重组DNA，转化细胞及其制造方法光学活性N-CARBAMYLAMINOSÄUREOR光学活性氨基
公开(公告)号：EP1852510A1
公开(公告)日：2007-11-07
申请号：EP06712435.4
申请日：2006-01-26
申请人： Kaneka Corporation
发明人： NISHI, Kenichi , YANAGISAWA, Satohiro , NANBA, Hirokazu , UEDA, Makoto , NORO, Naoto
IPC分类号： C12N15/61 , C12N1/21 , C12N9/90 , C12P17/10 , C12P41/00
CPC分类号： C12P13/04 , C12N9/90 , C12P41/009
摘要： Disclosed are a novel hydantoin racemase and a process for producing an optically active N-carbamylamino acid or an optically active amino acid using the hydantoin racemase. A novel hydantoin racemase isolated and purified from Bacillus sp. Strain KNK519HR; a gene encoding the hydantoin racemase; a recombinant plasmid having the gene introduced therein; a transformant having the hydantoin racemase gene introduced therein; and a process for producing an optically active N-carbamylamino acid or an optically active amino acid characterized in that a 5-substituted hydantoin compound is treated in the presence of hydantoinase and N-carbamylamino acid amidohydolase as well as the hydantoin racemase.
摘要翻译：本发明公开了一种新颖的乙内酰脲消旋酶和用于在光学活性N- carbamylamino酸或使用乙内酰脲消旋光学活性的氨基酸的生产的方法。一种新颖的乙内酰脲消旋酶分离和来自芽孢杆菌属的纯化。应变KNK519HR; 编码乙内酰脲消旋酶的基因; 具有该基因的重组质粒引入其中; 具有乙内酰脲消旋酶基因的转化体引入其中; 并在光学活性N- carbamylamino酸或其特征在于光学活性的氨基酸的生产过程做了5 substituiertem乙内酰脲化合物在乙内酰脲和N- carbamylamino酸的存在下处理amidohydolase以及乙内酰脲消旋酶。

6. 发明公开

EP1449923A1 NOVEL FORMATE DEHYDROGENASE TOLERANT TO HALOGEN COMPOUNDS AND PROCESS FOR PRODUCING THE SAME 有权
标题翻译： NEUE，GEGENÜBERHALOGENVERBINDUNGEN TOLERANTE FORMIATDEHYDROGENASE UND VERFAHREN ZUR HERSTELLUNG DAVON
公开(公告)号：EP1449923A1
公开(公告)日：2004-08-25
申请号：EP02800791.2
申请日：2002-10-09
申请人： KANEKA CORPORATION
发明人： NANBA, Hirokazu , TAKAOKA, Yasuko Sanseiso
IPC分类号： C12N15/53 , C12N9/04 , C12N1/21 , C12P21/02
CPC分类号： C12N9/0008
摘要： The invention provides a formate dehydrogenase having high specific activity, small Km values for formate and NAD, broad temperature and pH ranges of stability, broad temperature and pH ranges for action, and capable of regenerating a coenzyme with good efficiency in case of being present in an enzymatic reduction reaction system without inactivated.
The invention provides the enzyme mentioned above which has characteristics suited for industrial application by screening for soil formate dehydrogenase-producing microorganisms. The invention further provides a DNA containing the gene coding for the enzyme of the invention, a recombinant DNA constructed using a vector, and a transformant obtained by using this plasmid. The invention further provides a process for producing the formate dehydrogenase of the invention by using any microorganism of the genus Thiobacillus or a transformant constructed by using a formate dehydrogenase gene derived from above strain of microorganism, and a process for regenerating a coenzyme by using said enzyme in an enzymatic reduction system.
摘要翻译：本发明提供了具有高比活性，甲酸和NAD的小Km值的甲酸脱氢酶，稳定性的宽温度和pH范围，用于作用的宽温度和pH范围，并且能够在存在的情况下以良好的效率再生辅酶没有灭活的酶还原反应体系。本发明提供了通过筛选产生土壤甲酸脱氢酶的微生物而具有适用于工业应用的特征的上述酶。本发明还提供含有编码本发明酶的基因的DNA，使用载体构建的重组DNA和使用该质粒得到的转化体。本发明还提供了通过使用硫杆菌属的任何微生物或通过使用源自微生物的上述菌株的甲酸脱氢酶基因构建的转化体来生产本发明的甲酸脱氢酶的方法，以及通过使用所述酶再生辅酶的方法在酶促还原系统中。

7. 发明授权

EP1449923B1 NOVEL FORMATE DEHYDROGENASE TOLERANT TO HALOGEN COMPOUNDS AND PROCESS FOR PRODUCING THE SAME 有权
标题翻译：新的对面，卤素化合物及其制造容错甲酸脱氢酶和方法
公开(公告)号：EP1449923B1
公开(公告)日：2009-09-16
申请号：EP02800791.2
申请日：2002-10-09
申请人： KANEKA CORPORATION
发明人： NANBA, Hirokazu , TAKAOKA, Yasuko Sanseiso
IPC分类号： C12N15/53 , C12N9/04 , C12N1/21 , C12P21/02 , C12R1/01 , C12R1/19
CPC分类号： C12N9/0008
摘要： It is intended to provide a formate dehydrogenase which has a high specific activity, a small Km values to formic acid and NAD, a temperature-stability over a wide range, a pH-stability over a wide range, a wide function temperature range and a wide function pH range, is not inactivated even in case of coexisting in an enzymatic reduction system of a haloketone compound, and can efficiently regenerate a coenzyme. A formate dehydrogenase-producing strain is searched for from soil and an enzyme having the above-described properties and suitable for industrial use is provided. Moreover, a DNA containing the gene of this enzyme, a recombinant DNA with a vector and its transformant constructed by using a plasmid are provided. Furthermore, a process for producing the formate dehydrogenase using a strain belonging to the genus Thiobacillus or a transformant constructed by using a formate dehydrogenase gene originating in this strain, and a method of regenerating a coenzyme in an enzymatic reduction system with the use of this enzyme are provided.

8. 发明公开

EP1918375A1 D-AMINO ACID OXIDASE, AND METHOD FOR PRODUCTION OF L-AMINO ACID, 2-OXO ACID OR CYCLIC IMINE 有权转让
标题翻译： D-AMINOSÄURE-OXIDASE UND VERFAHREN ZUR HERSTELLUNG VON L-AMINOSÄURE，2-OXOSÄUREODER CYCLISCHEM IMIN
公开(公告)号：EP1918375A1
公开(公告)日：2008-05-07
申请号：EP06782154.6
申请日：2006-08-02
申请人： Kaneka Corporation
发明人： KANAMARU, Hiroyuki , UEDA, Makoto , MIKI, Ryuuji , NANBA, Hirokazu
IPC分类号： C12N15/09 , C12N1/15 , C12N1/21 , C12N9/06 , C12P7/40 , C12P13/04 , C12P17/10 , C12R1/19 , C12R1/72
CPC分类号： C12P7/40 , C12N9/0024 , C12P13/04 , C12P17/10
摘要： The present invention relates to novel D-amino acid oxidase isolated and purified from Candida intermedia, a gene encoding the D-amino acid oxidase, a recombinant plasmid containing the gene, and a transformant into which the D-amino acid oxidase gene has been introduced, as well as a production method of D-amino acid oxidase including culturing the transformant. Moreover, the present invention relates to a production method of L-amino acids, 2-oxo acids or cyclic imines, which include reacting racemic amino acids with the D-amino acid oxidase, more preferably, a production method of L-amino acids, which includes reacting racemic amino acid with the D-amino acid oxidase, amino acid dehydrogenase and an enzyme having a coenzyme-regenerating activity. According to the present invention, L-amino acids, 2-oxo acids or cyclic imines can be produced with good efficiency in an industrial scale.
摘要翻译：本发明涉及从念珠菌中间体分离纯化的新型D-氨基酸氧化酶，编码D-氨基酸氧化酶的基因，含有该基因的重组质粒，以及引入了D-氨基酸氧化酶基因的转化体以及D-氨基酸氧化酶的制造方法，包括培养转化体。此外，本发明涉及L-氨基酸，2-氧代酸或环状亚胺的制造方法，其包括使外消旋氨基酸与D-氨基酸氧化酶反应，更优选L-氨基酸的制造方法，其包括使外消旋氨基酸与D-氨基酸氧化酶，氨基酸脱氢酶和具有辅酶再生活性的酶反应。根据本发明，可以以工业规模高效率地生产L-氨基酸，2-氧酸或环亚胺。

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