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1. 发明公开

EP2241621A1 MODIFIED GLUCOSE DEHYDROGENASE GENE 有权
标题翻译：改性葡萄糖苷
公开(公告)号：EP2241621A1
公开(公告)日：2010-10-20
申请号：EP08866874.4
申请日：2008-12-26
申请人： Ikeda Food Research Co. Ltd.
发明人： HONDA, Michinari , TAKENAKA, Ryo , KOBAYASHI, Fuminao
IPC分类号： C12N15/09 , C12M1/34 , C12N1/15 , C12N9/04 , C12Q1/32
CPC分类号： C12Q1/32 , C12N9/0006 , C12Q1/006
摘要： [Object] To provide an FAD-conjugated glucose dehydrogenase having a higher specificity for glucose.
[Means for Resolution] A modified glucose dehydrogenase (GLD) which includes a substitution of at least one amino acid residue selected from the group consisting of amino acid residues at positions 37, 69, 72, 73, 76, 78, 102, 217, 228, 240, 356, 407, 424, 437, 527, and 530 in an amino acid sequence of a wild-type FAD-conjugated glucose dehydrogenase (GLD) represented by SEQ ID NO: 1, and has a decreased ratio of activity for xylose/activity for glucose as compared with the wild-type GLD; a polynucleotide encoding the modified GLD; a recombinant vector containing the polynucleotide; a transformed cell produced by using the recombinant vector; etc.
摘要翻译：提供对葡萄糖具有较高特异性的FAD-缀合的葡萄糖脱氢酶。 [解决方法]一种修饰的葡萄糖脱氢酶（GLD），其包括至少一个氨基酸残基的取代，所述氨基酸残基选自第37,69,72,73,76,78,102,217，在由SEQ ID NO：1表示的野生型FAD-缀合的葡萄糖脱氢酶（GLD）的氨基酸序列中的228,240,356,407,424,437,527和530，并且具有降低的与野生型GLD相比，葡萄糖的木糖/活性; 编码经修饰的GLD的多核苷酸; 含有多核苷酸的重组载体; 通过使用重组载体产生的转化细胞; 等等

2. 发明授权

EP2241621B1 MODIFIED GLUCOSE DEHYDROGENASE GENE 有权
标题翻译：修饰的葡萄糖脱氢酶基因
公开(公告)号：EP2241621B1
公开(公告)日：2012-08-15
申请号：EP08866874.4
申请日：2008-12-26
申请人： Ikeda Food Research Co. Ltd.
发明人： HONDA, Michinari , TAKENAKA, Ryo , KOBAYASHI, Fuminao
IPC分类号： C12Q1/00 , C12N9/04 , C12Q1/32
CPC分类号： C12Q1/32 , C12N9/0006 , C12Q1/006
摘要： It is intended to provide a FAD-conjugated glucose dehydrogenase having higher specificity for glucose. Modified GLD which includes substitution of at least one amino acid residue selected from the group consisting of amino acids at positions 37, 69, 72, 73, 76, 78, 102, 217, 228, 240, 356, 407, 424, 437, 527 and 530 in an amino acid sequence of a wild-type FAD-conjugated glucose dehydrogenase (GLD) represented by SEQ ID NO: 1, and has a decreased ratio of action to xylose/action to glucose as compared with wild-type GLD, a polynucleotide encoding the modified GLD, a recombinant vector containing the polynucleotide, and a transformed cell produced by using the recombinant vector, etc.
摘要翻译：旨在提供对葡萄糖具有更高特异性的FAD缀合的葡萄糖脱氢酶。修饰的GLD，其包括在位置37,69,72,73,76,78,102,217,228,240,356,407,424,437,418和478处的至少一个氨基酸残基的取代，所述氨基酸残基选自由以下氨基酸组成的组： 527和530中所示的野生型FAD-缀合的葡萄糖脱氢酶（GLD）的氨基酸序列中，并且与野生型GLD相比，其对木糖的作用/作用的比例降低，编码修饰GLD的多核苷酸，含有该多核苷酸的重组载体，以及使用该重组载体等制备的转化细胞等。

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